Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts

Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts

Abstract Adipocytes and adipose tissue derived cells have been investigated for their potential to contribute to the wound healing process. However, the details of how these cells interact with other essential cell types, such as myofibroblasts/fibroblasts, remain unclear. Using a novel in-vitro 3D...

Full description

Bibliographic Details
Main Authors: Mariam Y. El-Hattab, Yoshiaki Nagumo, Francoise A. Gourronc, Aloysius J. Klingelhutz, James A. Ankrum, Edward A. Sander
Format: Article
Language:English
Published: Nature Publishing Group 2020-06-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-020-67175-3
id doaj-2fcb313c25764006b3e282c1e69f58e8
record_format Article
spelling doaj-2fcb313c25764006b3e282c1e69f58e82021-06-27T11:38:51ZengNature Publishing GroupScientific Reports2045-23222020-06-0110111110.1038/s41598-020-67175-3Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to MyofibroblastsMariam Y. El-Hattab0Yoshiaki Nagumo1Francoise A. Gourronc2Aloysius J. Klingelhutz3James A. Ankrum4Edward A. Sander5Roy J. Carver Department of Biomedical Engineering, College of Engineering, University of IowaRoy J. Carver Department of Biomedical Engineering, College of Engineering, University of IowaDepartment of Microbiology and Immunology, Carver College of Medicine, University of IowaDepartment of Microbiology and Immunology, Carver College of Medicine, University of IowaRoy J. Carver Department of Biomedical Engineering, College of Engineering, University of IowaRoy J. Carver Department of Biomedical Engineering, College of Engineering, University of IowaAbstract Adipocytes and adipose tissue derived cells have been investigated for their potential to contribute to the wound healing process. However, the details of how these cells interact with other essential cell types, such as myofibroblasts/fibroblasts, remain unclear. Using a novel in-vitro 3D human adipocyte/pre-adipocyte spheroid model, we investigated whether adipocytes and their precursors (pre-adipocytes) secrete factors that affect human dermal fibroblast behavior. We found that both adipocyte and pre-adipocyte conditioned medium induced the migration of fibroblasts, but only adipocyte conditioned medium induced fibroblast differentiation into a highly contractile, collagen producing myofibroblast phenotype. Furthermore, adipocyte mediated myofibroblast induction occurred through a TGF-β independent mechanism. Our findings contribute to a better understanding on the involvement of adipose tissue in wound healing, and may help to uncover and develop fat-related wound healing treatments.https://doi.org/10.1038/s41598-020-67175-3
collection DOAJ
language English
format Article
sources DOAJ
author Mariam Y. El-Hattab
Yoshiaki Nagumo
Francoise A. Gourronc
Aloysius J. Klingelhutz
James A. Ankrum
Edward A. Sander
spellingShingle Mariam Y. El-Hattab
Yoshiaki Nagumo
Francoise A. Gourronc
Aloysius J. Klingelhutz
James A. Ankrum
Edward A. Sander
Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts
Scientific Reports
author_facet Mariam Y. El-Hattab
Yoshiaki Nagumo
Francoise A. Gourronc
Aloysius J. Klingelhutz
James A. Ankrum
Edward A. Sander
author_sort Mariam Y. El-Hattab
title Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts
title_short Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts
title_full Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts
title_fullStr Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts
title_full_unstemmed Human Adipocyte Conditioned Medium Promotes In Vitro Fibroblast Conversion to Myofibroblasts
title_sort human adipocyte conditioned medium promotes in vitro fibroblast conversion to myofibroblasts
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2020-06-01
description Abstract Adipocytes and adipose tissue derived cells have been investigated for their potential to contribute to the wound healing process. However, the details of how these cells interact with other essential cell types, such as myofibroblasts/fibroblasts, remain unclear. Using a novel in-vitro 3D human adipocyte/pre-adipocyte spheroid model, we investigated whether adipocytes and their precursors (pre-adipocytes) secrete factors that affect human dermal fibroblast behavior. We found that both adipocyte and pre-adipocyte conditioned medium induced the migration of fibroblasts, but only adipocyte conditioned medium induced fibroblast differentiation into a highly contractile, collagen producing myofibroblast phenotype. Furthermore, adipocyte mediated myofibroblast induction occurred through a TGF-β independent mechanism. Our findings contribute to a better understanding on the involvement of adipose tissue in wound healing, and may help to uncover and develop fat-related wound healing treatments.
url https://doi.org/10.1038/s41598-020-67175-3
work_keys_str_mv AT mariamyelhattab humanadipocyteconditionedmediumpromotesinvitrofibroblastconversiontomyofibroblasts
AT yoshiakinagumo humanadipocyteconditionedmediumpromotesinvitrofibroblastconversiontomyofibroblasts
AT francoiseagourronc humanadipocyteconditionedmediumpromotesinvitrofibroblastconversiontomyofibroblasts
AT aloysiusjklingelhutz humanadipocyteconditionedmediumpromotesinvitrofibroblastconversiontomyofibroblasts
AT jamesaankrum humanadipocyteconditionedmediumpromotesinvitrofibroblastconversiontomyofibroblasts
AT edwardasander humanadipocyteconditionedmediumpromotesinvitrofibroblastconversiontomyofibroblasts
_version_ 1721357494159147008

Similar Items