Comparison of protective effect of ordinary Cordyceps militaris and selenium-enriched Cordyceps militaris on triptolide-induced acute hepatotoxicity and the potential mechanisms
This study aimed to investigate the prophylactic effect of selenium-enriched Cordyceps militaris (CS) against triptolide-induced acute hepatotoxicity. Rats were intragastrically administrated with CS and ordinary Cordyceps militaris (CM) for 7 consecutive days prior to triptolide intoxication. Tript...
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doaj-300363d87c7c4f729933484b7d5f2a5e2021-04-30T07:13:49ZengElsevierJournal of Functional Foods1756-46462018-07-0146365377Comparison of protective effect of ordinary Cordyceps militaris and selenium-enriched Cordyceps militaris on triptolide-induced acute hepatotoxicity and the potential mechanismsLan Wang0Qiong-hui Huang1Yan-feng Huang2Jian-hui Xie3Chang Qu4Jian-ping Chen5Lin Zheng6Tie-gang Yi7Hui-fang Zeng8Hui-lin Li9Department of Pharmacy, The First Affiliated Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, PR ChinaGuangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, PR ChinaGuangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, PR ChinaGuangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, PR ChinaGuangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, PR ChinaShenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, ChinaShenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, ChinaShenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, ChinaDepartment of Pharmacy, The First Affiliated Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, PR China; Corresponding authors.Shenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, China; Corresponding authors.This study aimed to investigate the prophylactic effect of selenium-enriched Cordyceps militaris (CS) against triptolide-induced acute hepatotoxicity. Rats were intragastrically administrated with CS and ordinary Cordyceps militaris (CM) for 7 consecutive days prior to triptolide intoxication. Triptolide elevated levels of serum aminotransferases, total cholesterol and caused severe liver injury. However, CS remarkably inhibited the increases of these parameters and pathological damages. The action mechanisms might be associated with its antioxidant property by enhancing antioxidant enzymes activities and triggering nuclear factor E2-related factor 2 (Nrf2) translocation and downstream genes expression. Besides, CS prevented cellular apoptosis through up-regulating Bcl-2/Bax ratio and down-regulating cytochrome c and caspase-3 cleavage. Constituent analyses showed that CS had higher amounts of cordycepin, selenium, selenocystine and polysaccharides than CM. In conclusion, CS effectively prevented triptolide-induced hepatotoxicity via activating Nrf2 pathway and inhibiting hepatocyte apoptosis, suggesting that it could be a promising hepatic protector and a befitting nutraceutical supplement.http://www.sciencedirect.com/science/article/pii/S1756464618302330Selenium-enriched Cordyceps militarisTriptolideHepatotoxicityAntioxidativeNrf2 pathwayAnti-apoptosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lan Wang Qiong-hui Huang Yan-feng Huang Jian-hui Xie Chang Qu Jian-ping Chen Lin Zheng Tie-gang Yi Hui-fang Zeng Hui-lin Li |
spellingShingle |
Lan Wang Qiong-hui Huang Yan-feng Huang Jian-hui Xie Chang Qu Jian-ping Chen Lin Zheng Tie-gang Yi Hui-fang Zeng Hui-lin Li Comparison of protective effect of ordinary Cordyceps militaris and selenium-enriched Cordyceps militaris on triptolide-induced acute hepatotoxicity and the potential mechanisms Journal of Functional Foods Selenium-enriched Cordyceps militaris Triptolide Hepatotoxicity Antioxidative Nrf2 pathway Anti-apoptosis |
author_facet |
Lan Wang Qiong-hui Huang Yan-feng Huang Jian-hui Xie Chang Qu Jian-ping Chen Lin Zheng Tie-gang Yi Hui-fang Zeng Hui-lin Li |
author_sort |
Lan Wang |
title |
Comparison of protective effect of ordinary Cordyceps militaris and selenium-enriched Cordyceps militaris on triptolide-induced acute hepatotoxicity and the potential mechanisms |
title_short |
Comparison of protective effect of ordinary Cordyceps militaris and selenium-enriched Cordyceps militaris on triptolide-induced acute hepatotoxicity and the potential mechanisms |
title_full |
Comparison of protective effect of ordinary Cordyceps militaris and selenium-enriched Cordyceps militaris on triptolide-induced acute hepatotoxicity and the potential mechanisms |
title_fullStr |
Comparison of protective effect of ordinary Cordyceps militaris and selenium-enriched Cordyceps militaris on triptolide-induced acute hepatotoxicity and the potential mechanisms |
title_full_unstemmed |
Comparison of protective effect of ordinary Cordyceps militaris and selenium-enriched Cordyceps militaris on triptolide-induced acute hepatotoxicity and the potential mechanisms |
title_sort |
comparison of protective effect of ordinary cordyceps militaris and selenium-enriched cordyceps militaris on triptolide-induced acute hepatotoxicity and the potential mechanisms |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2018-07-01 |
description |
This study aimed to investigate the prophylactic effect of selenium-enriched Cordyceps militaris (CS) against triptolide-induced acute hepatotoxicity. Rats were intragastrically administrated with CS and ordinary Cordyceps militaris (CM) for 7 consecutive days prior to triptolide intoxication. Triptolide elevated levels of serum aminotransferases, total cholesterol and caused severe liver injury. However, CS remarkably inhibited the increases of these parameters and pathological damages. The action mechanisms might be associated with its antioxidant property by enhancing antioxidant enzymes activities and triggering nuclear factor E2-related factor 2 (Nrf2) translocation and downstream genes expression. Besides, CS prevented cellular apoptosis through up-regulating Bcl-2/Bax ratio and down-regulating cytochrome c and caspase-3 cleavage. Constituent analyses showed that CS had higher amounts of cordycepin, selenium, selenocystine and polysaccharides than CM. In conclusion, CS effectively prevented triptolide-induced hepatotoxicity via activating Nrf2 pathway and inhibiting hepatocyte apoptosis, suggesting that it could be a promising hepatic protector and a befitting nutraceutical supplement. |
topic |
Selenium-enriched Cordyceps militaris Triptolide Hepatotoxicity Antioxidative Nrf2 pathway Anti-apoptosis |
url |
http://www.sciencedirect.com/science/article/pii/S1756464618302330 |
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