Cytogenetics and stripe rust resistance of wheat–Thinopyrum elongatum hybrid derivatives
Abstract Background Amphidiploids generated by distant hybridization are commonly used as genetic bridge to transfer desirable genes from wild wheat species into cultivated wheat. This method is typically used to enhance the resistance of wheat to biotic or abiotic stresses, and to increase crop yie...
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doaj-300f86c2d18b4ae89b35f9423230a63d2020-11-24T22:20:05ZengBMCMolecular Cytogenetics1755-81662018-02-011111910.1186/s13039-018-0366-4Cytogenetics and stripe rust resistance of wheat–Thinopyrum elongatum hybrid derivativesDaiyan Li0Dan Long1Tinghui Li2Yanli Wu3Yi Wang4Jian Zeng5Lili Xu6Xing Fan7Lina Sha8Haiqin Zhang9Yonghong Zhou10Houyang Kang11Triticeae Research Institute, Sichuan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityCollege of Resources, Sichuan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityAbstract Background Amphidiploids generated by distant hybridization are commonly used as genetic bridge to transfer desirable genes from wild wheat species into cultivated wheat. This method is typically used to enhance the resistance of wheat to biotic or abiotic stresses, and to increase crop yield and quality. Tetraploid Thinopyrum elongatum exhibits strong adaptability, resistance to stripe rust and Fusarium head blight, and tolerance to salt, drought, and cold. Results In the present study, we produced hybrid derivatives by crossing and backcrossing the Triticum durum–Th. elongatum partial amphidiploid (Trititrigia 8801, 2n = 6× = 42, AABBEE) with wheat cultivars common to the Sichuan Basin. By means of cytogenetic and disease resistance analyses, we identified progeny harboring alien chromosomes and measured their resistance to stripe rust. Hybrid progenies possessed chromosome numbers ranging from 40 to 47 (mean = 42.72), with 40.0% possessing 42 chromosomes. Genomic in situ hybridization revealed that the number of alien chromosomes ranged from 1 to 11. Out of the 50 of analyzed lines, five represented chromosome addition (2n = 44 = 42 W + 2E) and other five were chromosome substitution lines (2n = 42 = 40 W + 2E). Importantly, a single chromosome derived from wheat–Th. elongatum intergenomic Robertsonian translocations chromosome was occurred in 12 lines. Compared with the wheat parental cultivars (‘CN16’ and ‘SM482’), the majority (70%) of the derivative lines were highly resistant to strains of stripe rust pathogen known to be prevalent in China. Conclusion The findings suggest that these hybrid-derivative lines with stripe rust resistance could potentially be used as germplasm sources for further wheat improvement.http://link.springer.com/article/10.1186/s13039-018-0366-4Genomic in situ hybridizationStripe rustThinopyrum elongatumHybrid progeny |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Daiyan Li Dan Long Tinghui Li Yanli Wu Yi Wang Jian Zeng Lili Xu Xing Fan Lina Sha Haiqin Zhang Yonghong Zhou Houyang Kang |
spellingShingle |
Daiyan Li Dan Long Tinghui Li Yanli Wu Yi Wang Jian Zeng Lili Xu Xing Fan Lina Sha Haiqin Zhang Yonghong Zhou Houyang Kang Cytogenetics and stripe rust resistance of wheat–Thinopyrum elongatum hybrid derivatives Molecular Cytogenetics Genomic in situ hybridization Stripe rust Thinopyrum elongatum Hybrid progeny |
author_facet |
Daiyan Li Dan Long Tinghui Li Yanli Wu Yi Wang Jian Zeng Lili Xu Xing Fan Lina Sha Haiqin Zhang Yonghong Zhou Houyang Kang |
author_sort |
Daiyan Li |
title |
Cytogenetics and stripe rust resistance of wheat–Thinopyrum elongatum hybrid derivatives |
title_short |
Cytogenetics and stripe rust resistance of wheat–Thinopyrum elongatum hybrid derivatives |
title_full |
Cytogenetics and stripe rust resistance of wheat–Thinopyrum elongatum hybrid derivatives |
title_fullStr |
Cytogenetics and stripe rust resistance of wheat–Thinopyrum elongatum hybrid derivatives |
title_full_unstemmed |
Cytogenetics and stripe rust resistance of wheat–Thinopyrum elongatum hybrid derivatives |
title_sort |
cytogenetics and stripe rust resistance of wheat–thinopyrum elongatum hybrid derivatives |
publisher |
BMC |
series |
Molecular Cytogenetics |
issn |
1755-8166 |
publishDate |
2018-02-01 |
description |
Abstract Background Amphidiploids generated by distant hybridization are commonly used as genetic bridge to transfer desirable genes from wild wheat species into cultivated wheat. This method is typically used to enhance the resistance of wheat to biotic or abiotic stresses, and to increase crop yield and quality. Tetraploid Thinopyrum elongatum exhibits strong adaptability, resistance to stripe rust and Fusarium head blight, and tolerance to salt, drought, and cold. Results In the present study, we produced hybrid derivatives by crossing and backcrossing the Triticum durum–Th. elongatum partial amphidiploid (Trititrigia 8801, 2n = 6× = 42, AABBEE) with wheat cultivars common to the Sichuan Basin. By means of cytogenetic and disease resistance analyses, we identified progeny harboring alien chromosomes and measured their resistance to stripe rust. Hybrid progenies possessed chromosome numbers ranging from 40 to 47 (mean = 42.72), with 40.0% possessing 42 chromosomes. Genomic in situ hybridization revealed that the number of alien chromosomes ranged from 1 to 11. Out of the 50 of analyzed lines, five represented chromosome addition (2n = 44 = 42 W + 2E) and other five were chromosome substitution lines (2n = 42 = 40 W + 2E). Importantly, a single chromosome derived from wheat–Th. elongatum intergenomic Robertsonian translocations chromosome was occurred in 12 lines. Compared with the wheat parental cultivars (‘CN16’ and ‘SM482’), the majority (70%) of the derivative lines were highly resistant to strains of stripe rust pathogen known to be prevalent in China. Conclusion The findings suggest that these hybrid-derivative lines with stripe rust resistance could potentially be used as germplasm sources for further wheat improvement. |
topic |
Genomic in situ hybridization Stripe rust Thinopyrum elongatum Hybrid progeny |
url |
http://link.springer.com/article/10.1186/s13039-018-0366-4 |
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