Methodology of Selecting the Optimal Receptor to Create an Electrochemical Immunosensor for Equine Arteritis Virus Protein Detection

The study reports a methodology of selecting the optimal receptor to create an electrochemical immunosensor for equine arteritis virus (EAV) protein detection. The detection was based on antigen recognition by antibodies immobilized on gold electrodes. Modification steps were controlled by electroch...

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Bibliographic Details
Main Authors: Mateusz Brodowski, Marcin Kowalski, Wioleta Białobrzeska, Katarzyna Pałka, Rafał Walkusz, Justyna Roguszczak, Tomasz Łęga, Marta Sosnowska, Małgorzata Biedulska, Joanna Kreczko Kurzawa, Ewelina Bięga, Joanna Wysocka, Marta Lisowska, Katarzyna Niedźwiedzka, Tomasz Lipiński, Sabina Żołędowska, Dawid Nidzworski
Format: Article
Language:English
Published: MDPI AG 2021-09-01
Series:Chemosensors
Subjects:
Online Access:https://www.mdpi.com/2227-9040/9/9/265
Description
Summary:The study reports a methodology of selecting the optimal receptor to create an electrochemical immunosensor for equine arteritis virus (EAV) protein detection. The detection was based on antigen recognition by antibodies immobilized on gold electrodes. Modification steps were controlled by electrochemical impedance spectroscopy and cyclic voltammetry measurements. In order to obtain the impedance immunosensor with the best parameters, seven different receptors complementary to equine arteritis virus protein were used. In order to make the selection, a rapid screening test was carried out to check the sensor’s response to blank, extremely low and high concentrations of target EAV protein, and negative sample: M protein from <i>Streptococcus equi</i> and glycoprotein G from <i>Equid alphaherpesvirus</i> 1. F6 10G receptor showed the best performance.
ISSN:2227-9040