Pooled library screening with multiplexed Cpf1 library

AsCpf1 is an alternative nuclease to Cas9 for CRISPR mediated genome engineering. Here the authors demonstrate functional genomic screens with AsCpf1 that minimize library size with no loss in gene targeting efficiency.

Bibliographic Details
Main Authors:	Jintan Liu, Sanjana Srinivasan, Chieh-Yuan Li, I-Lin Ho, Johnathon Rose, MennatAllah Shaheen, Gang Wang, Wantong Yao, Angela Deem, Chris Bristow, Traver Hart, Giulio Draetta
Format:	Article
Language:	English
Published:	Nature Publishing Group 2019-07-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-019-10963-x

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spelling	doaj-302d4902b46145a7a871a5d3d68469702021-05-11T11:49:53ZengNature Publishing GroupNature Communications2041-17232019-07-0110111010.1038/s41467-019-10963-xPooled library screening with multiplexed Cpf1 libraryJintan Liu0Sanjana Srinivasan1Chieh-Yuan Li2I-Lin Ho3Johnathon Rose4MennatAllah Shaheen5Gang Wang6Wantong Yao7Angela Deem8Chris Bristow9Traver Hart10Giulio Draetta11Department of Genomic Medicine, The University of Texas MD Anderson Cancer CenterDepartment of Genomic Medicine, The University of Texas MD Anderson Cancer CenterDepartment of Genomic Medicine, The University of Texas MD Anderson Cancer CenterDepartment of Genomic Medicine, The University of Texas MD Anderson Cancer CenterDepartment of Genomic Medicine, The University of Texas MD Anderson Cancer CenterDepartment of Genomic Medicine, The University of Texas MD Anderson Cancer CenterDepartment of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer CenterDepartment of Genomic Medicine, The University of Texas MD Anderson Cancer CenterInstitute for Applied Cancer Science, The University of Texas MD Anderson Cancer CenterInstitute for Applied Cancer Science, The University of Texas MD Anderson Cancer CenterDepartment of Bioinformatics and Computational Biology, The University of Texas MD Anderson Cancer CenterDepartment of Genomic Medicine, The University of Texas MD Anderson Cancer CenterAsCpf1 is an alternative nuclease to Cas9 for CRISPR mediated genome engineering. Here the authors demonstrate functional genomic screens with AsCpf1 that minimize library size with no loss in gene targeting efficiency.https://doi.org/10.1038/s41467-019-10963-x
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Jintan Liu Sanjana Srinivasan Chieh-Yuan Li I-Lin Ho Johnathon Rose MennatAllah Shaheen Gang Wang Wantong Yao Angela Deem Chris Bristow Traver Hart Giulio Draetta
spellingShingle	Jintan Liu Sanjana Srinivasan Chieh-Yuan Li I-Lin Ho Johnathon Rose MennatAllah Shaheen Gang Wang Wantong Yao Angela Deem Chris Bristow Traver Hart Giulio Draetta Pooled library screening with multiplexed Cpf1 library Nature Communications
author_facet	Jintan Liu Sanjana Srinivasan Chieh-Yuan Li I-Lin Ho Johnathon Rose MennatAllah Shaheen Gang Wang Wantong Yao Angela Deem Chris Bristow Traver Hart Giulio Draetta
author_sort	Jintan Liu
title	Pooled library screening with multiplexed Cpf1 library
title_short	Pooled library screening with multiplexed Cpf1 library
title_full	Pooled library screening with multiplexed Cpf1 library
title_fullStr	Pooled library screening with multiplexed Cpf1 library
title_full_unstemmed	Pooled library screening with multiplexed Cpf1 library
title_sort	pooled library screening with multiplexed cpf1 library
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2019-07-01
description	AsCpf1 is an alternative nuclease to Cas9 for CRISPR mediated genome engineering. Here the authors demonstrate functional genomic screens with AsCpf1 that minimize library size with no loss in gene targeting efficiency.
url	https://doi.org/10.1038/s41467-019-10963-x
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Pooled library screening with multiplexed Cpf1 library

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