The First Whole Genome Sequence and Characterisation of Avian Nephritis Virus Genotype 3

Avian nephritis virus (ANV) is classified in the <i>Avastroviridae</i> family with disease associations with nephritis, uneven flock growth and runting stunting syndrome (RSS) in chicken and turkey flocks, and other avian species. The whole genome of ANV genotype 3 (ANV-3) of 6959 nucleo...

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Main Authors:	Paula Lagan Tregaskis, Ryan Devaney, Victoria J. Smyth
Format:	Article
Language:	English
Published:	MDPI AG 2021-02-01
Series:	Viruses
Subjects:	avian nephritis virus astrovirus phylogenetics molecular characterisation genotyping and recombination
Online Access:	https://www.mdpi.com/1999-4915/13/2/235

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spelling	doaj-303f8bb2e1084a2aafa3045c97415da52021-02-04T00:01:50ZengMDPI AGViruses1999-49152021-02-011323523510.3390/v13020235The First Whole Genome Sequence and Characterisation of Avian Nephritis Virus Genotype 3Paula Lagan Tregaskis0Ryan Devaney1Victoria J. Smyth2Virology, Veterinary Science Division, Agri-Food and Biosciences Institute, Stormont, Belfast BT4 3SD, Northern Ireland, UKVirology, Veterinary Science Division, Agri-Food and Biosciences Institute, Stormont, Belfast BT4 3SD, Northern Ireland, UKVirology, Veterinary Science Division, Agri-Food and Biosciences Institute, Stormont, Belfast BT4 3SD, Northern Ireland, UKAvian nephritis virus (ANV) is classified in the <i>Avastroviridae</i> family with disease associations with nephritis, uneven flock growth and runting stunting syndrome (RSS) in chicken and turkey flocks, and other avian species. The whole genome of ANV genotype 3 (ANV-3) of 6959 nucleotides including the untranslated 5′ and 3′ regions and polyadenylated tail was detected in a metagenomic virome investigation of RSS-affected chicken broiler flocks. This report characterises the ANV-3 genome, identifying partially overlapping open reading frames (ORFs), ORF1a and ORF1b, and an opposing secondary pseudoknot prior to a ribosomal frameshift stemloop structure, with a separate ORF2, whilst observing conserved astrovirus motifs. Phylogenetic analysis of the <i>Avastroviridae</i> whole genome and ORF2 capsid polyprotein classified the first complete whole genome of ANV-3 within <i>Avastroviridae</i> genogroup 2.https://www.mdpi.com/1999-4915/13/2/235avian nephritis virusastrovirusphylogeneticsmolecular characterisationgenotyping and recombination
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Paula Lagan Tregaskis Ryan Devaney Victoria J. Smyth
spellingShingle	Paula Lagan Tregaskis Ryan Devaney Victoria J. Smyth The First Whole Genome Sequence and Characterisation of Avian Nephritis Virus Genotype 3 Viruses avian nephritis virus astrovirus phylogenetics molecular characterisation genotyping and recombination
author_facet	Paula Lagan Tregaskis Ryan Devaney Victoria J. Smyth
author_sort	Paula Lagan Tregaskis
title	The First Whole Genome Sequence and Characterisation of Avian Nephritis Virus Genotype 3
title_short	The First Whole Genome Sequence and Characterisation of Avian Nephritis Virus Genotype 3
title_full	The First Whole Genome Sequence and Characterisation of Avian Nephritis Virus Genotype 3
title_fullStr	The First Whole Genome Sequence and Characterisation of Avian Nephritis Virus Genotype 3
title_full_unstemmed	The First Whole Genome Sequence and Characterisation of Avian Nephritis Virus Genotype 3
title_sort	first whole genome sequence and characterisation of avian nephritis virus genotype 3
publisher	MDPI AG
series	Viruses
issn	1999-4915
publishDate	2021-02-01
description	Avian nephritis virus (ANV) is classified in the <i>Avastroviridae</i> family with disease associations with nephritis, uneven flock growth and runting stunting syndrome (RSS) in chicken and turkey flocks, and other avian species. The whole genome of ANV genotype 3 (ANV-3) of 6959 nucleotides including the untranslated 5′ and 3′ regions and polyadenylated tail was detected in a metagenomic virome investigation of RSS-affected chicken broiler flocks. This report characterises the ANV-3 genome, identifying partially overlapping open reading frames (ORFs), ORF1a and ORF1b, and an opposing secondary pseudoknot prior to a ribosomal frameshift stemloop structure, with a separate ORF2, whilst observing conserved astrovirus motifs. Phylogenetic analysis of the <i>Avastroviridae</i> whole genome and ORF2 capsid polyprotein classified the first complete whole genome of ANV-3 within <i>Avastroviridae</i> genogroup 2.
topic	avian nephritis virus astrovirus phylogenetics molecular characterisation genotyping and recombination
url	https://www.mdpi.com/1999-4915/13/2/235
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The First Whole Genome Sequence and Characterisation of Avian Nephritis Virus Genotype 3

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