Genome-Wide Identification, Evolution, and Expression Analysis of <i>TPS</i> and <i>TPP</i> Gene Families in <i>Brachypodium distachyon</i>
Trehalose biosynthesis enzyme homologues in plants contain two families, trehalose-6-phosphate synthases (TPSs) and trehalose-6-phosphate phosphatases (TPPs). Both families participate in trehalose synthesis and a variety of stress-resistance processes. Here, nine <i>BdTPS</i> and ten &l...
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doaj-304b9f1b64a14ee58fe417fc3078d3d82020-11-24T21:59:50ZengMDPI AGPlants2223-77472019-09-0181036210.3390/plants8100362plants8100362Genome-Wide Identification, Evolution, and Expression Analysis of <i>TPS</i> and <i>TPP</i> Gene Families in <i>Brachypodium distachyon</i>Song Wang0Kai Ouyang1Kai Wang2Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, ChinaKey Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, ChinaKey Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, ChinaTrehalose biosynthesis enzyme homologues in plants contain two families, trehalose-6-phosphate synthases (TPSs) and trehalose-6-phosphate phosphatases (TPPs). Both families participate in trehalose synthesis and a variety of stress-resistance processes. Here, nine <i>BdTPS</i> and ten <i>BdTPP</i> genes were identified based on the <i>Brachypodium distachyon</i> genome, and all genes were classified into three classes. The Class I and Class II members differed substantially in gene structures, conserved motifs, and protein sequence identities, implying varied gene functions. Gene duplication analysis showed that one <i>BdTPS</i> gene pair and four <i>BdTPP</i> gene pairs are formed by duplication events. The value of <i>Ka/Ks</i> (non-synonymous/synonymous) was less than 1, suggesting purifying selection in these gene families. The <i>cis</i>-elements and gene interaction network prediction showed that many family members may be involved in stress responses. The quantitative real-time reverse transcription (qRT-PCR) results further supported that most <i>BdTPSs</i> responded to at least one stress or abscisic acid (ABA) treatment, whereas over half of <i>BdTPPs</i> were downregulated after stress treatment, implying that <i>BdTPSs</i> play a more important role in stress responses than <i>BdTPPs</i>. This work provides a foundation for the genome-wide identification of the <i>B. distachyon TPS−TPP</i> gene families and a frame for further studies of these gene families in abiotic stress responses.https://www.mdpi.com/2223-7747/8/10/362TPSTPP<i>Brachypodium distachyon</i>abiotic stressexpression analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Song Wang Kai Ouyang Kai Wang |
spellingShingle |
Song Wang Kai Ouyang Kai Wang Genome-Wide Identification, Evolution, and Expression Analysis of <i>TPS</i> and <i>TPP</i> Gene Families in <i>Brachypodium distachyon</i> Plants TPS TPP <i>Brachypodium distachyon</i> abiotic stress expression analysis |
author_facet |
Song Wang Kai Ouyang Kai Wang |
author_sort |
Song Wang |
title |
Genome-Wide Identification, Evolution, and Expression Analysis of <i>TPS</i> and <i>TPP</i> Gene Families in <i>Brachypodium distachyon</i> |
title_short |
Genome-Wide Identification, Evolution, and Expression Analysis of <i>TPS</i> and <i>TPP</i> Gene Families in <i>Brachypodium distachyon</i> |
title_full |
Genome-Wide Identification, Evolution, and Expression Analysis of <i>TPS</i> and <i>TPP</i> Gene Families in <i>Brachypodium distachyon</i> |
title_fullStr |
Genome-Wide Identification, Evolution, and Expression Analysis of <i>TPS</i> and <i>TPP</i> Gene Families in <i>Brachypodium distachyon</i> |
title_full_unstemmed |
Genome-Wide Identification, Evolution, and Expression Analysis of <i>TPS</i> and <i>TPP</i> Gene Families in <i>Brachypodium distachyon</i> |
title_sort |
genome-wide identification, evolution, and expression analysis of <i>tps</i> and <i>tpp</i> gene families in <i>brachypodium distachyon</i> |
publisher |
MDPI AG |
series |
Plants |
issn |
2223-7747 |
publishDate |
2019-09-01 |
description |
Trehalose biosynthesis enzyme homologues in plants contain two families, trehalose-6-phosphate synthases (TPSs) and trehalose-6-phosphate phosphatases (TPPs). Both families participate in trehalose synthesis and a variety of stress-resistance processes. Here, nine <i>BdTPS</i> and ten <i>BdTPP</i> genes were identified based on the <i>Brachypodium distachyon</i> genome, and all genes were classified into three classes. The Class I and Class II members differed substantially in gene structures, conserved motifs, and protein sequence identities, implying varied gene functions. Gene duplication analysis showed that one <i>BdTPS</i> gene pair and four <i>BdTPP</i> gene pairs are formed by duplication events. The value of <i>Ka/Ks</i> (non-synonymous/synonymous) was less than 1, suggesting purifying selection in these gene families. The <i>cis</i>-elements and gene interaction network prediction showed that many family members may be involved in stress responses. The quantitative real-time reverse transcription (qRT-PCR) results further supported that most <i>BdTPSs</i> responded to at least one stress or abscisic acid (ABA) treatment, whereas over half of <i>BdTPPs</i> were downregulated after stress treatment, implying that <i>BdTPSs</i> play a more important role in stress responses than <i>BdTPPs</i>. This work provides a foundation for the genome-wide identification of the <i>B. distachyon TPS−TPP</i> gene families and a frame for further studies of these gene families in abiotic stress responses. |
topic |
TPS TPP <i>Brachypodium distachyon</i> abiotic stress expression analysis |
url |
https://www.mdpi.com/2223-7747/8/10/362 |
work_keys_str_mv |
AT songwang genomewideidentificationevolutionandexpressionanalysisofitpsianditppigenefamiliesinibrachypodiumdistachyoni AT kaiouyang genomewideidentificationevolutionandexpressionanalysisofitpsianditppigenefamiliesinibrachypodiumdistachyoni AT kaiwang genomewideidentificationevolutionandexpressionanalysisofitpsianditppigenefamiliesinibrachypodiumdistachyoni |
_version_ |
1725847031016062976 |