Bacillus thuringiensis in caterpillars and associated materials collected from protected tropical forests in northwestern Costa Rica
Bacillus thuringiensis (Bt) synthesizes crystalline inclusions that are toxic to caterpillars (Lepidoptera) and other orders of invertebrates. Materials associated with 37 caterpillars from 16 species, collected while feeding on 15 different species of host plants in dry, cloud and rain forests loca...
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Vicerractoría Investigación
2006-06-01
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doaj-308b95975bb34ed18efbb7168a01c6d82020-11-24T22:43:18ZengVicerractoría InvestigaciónRevista de Biología Tropical0034-77442215-20752006-06-01542265271Bacillus thuringiensis in caterpillars and associated materials collected from protected tropical forests in northwestern Costa RicaCésar Rodríguez-SánchezAna SittenfeldDaniel H JanzenAna M EspinozaBacillus thuringiensis (Bt) synthesizes crystalline inclusions that are toxic to caterpillars (Lepidoptera) and other orders of invertebrates. Materials associated with 37 caterpillars from 16 species, collected while feeding on 15 different species of host plants in dry, cloud and rain forests located in the Área de Conservación Guanacaste in northwestern Costa Rica, were examined for the presence of Bt. From a total of 101 derived samples, 25 Bt isolates were cultured: 56% from host plant leaves, 8% from caterpillar guts and 36% from caterpillar fecal pellets. Bt was isolated from at least one sample in 38% of the systems constituted by the food plant, gut and fecal pellets corresponding to a single caterpillar. Four different morphologies of crystalline inclusions were observed, with bipyramidal and irregular crystal morphologies being the most prevalent. Rev. Biol. Trop. 54(2): 265-271. Epub 2006 Jun 01.<br>Bacillus thuringiensis (Bt) sintetiza inclusiones cristalinas que resultan tóxicas para algunas larvas de lepidópteros y otros órdenes de invertebrados. Su presencia fue examinada en materiales asociados a 37 orugas de mariposas de 16 especies, las cuales fueron colectadas mientras se alimentaban en 15 especies diferentes de plantas hospederas en bosques secos, nubosos y húmedos localizados dentro del Área de Conservación Guanacaste (ACG) en el noroeste de Costa Rica. A partir de un total de 101 muestras se obtuvo 25 aislamientos de Bt: 56% a partir de material foliar de las plantas hospederas, 8% a partir del contenido intestinal de las larvas y 36% a partir de sus excrementos. Esta bacteria fue cultivada a partir de al menos uno de los 3 diferentes tipos de muestra asociados a una oruga particular (planta hospedera, intestino, excremento) en 38% de los casos posibles. En la colección de aislamientos obtenida se observaron cuatro morfologías de inclusiones cristalinas, siendo aquellas bipiramidales e irregulares las más prevalentes.http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442006000200004Bacillus thuringiensislarvas de mariposaplantas hospederastractos intestinales de insectosmaterial fecaláreas protegidasBacillus thuringiensiscaterpillarsfood plantsinsect gutsfecal pelletsconserved wildlands |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
César Rodríguez-Sánchez Ana Sittenfeld Daniel H Janzen Ana M Espinoza |
spellingShingle |
César Rodríguez-Sánchez Ana Sittenfeld Daniel H Janzen Ana M Espinoza Bacillus thuringiensis in caterpillars and associated materials collected from protected tropical forests in northwestern Costa Rica Revista de Biología Tropical Bacillus thuringiensis larvas de mariposa plantas hospederas tractos intestinales de insectos material fecal áreas protegidas Bacillus thuringiensis caterpillars food plants insect guts fecal pellets conserved wildlands |
author_facet |
César Rodríguez-Sánchez Ana Sittenfeld Daniel H Janzen Ana M Espinoza |
author_sort |
César Rodríguez-Sánchez |
title |
Bacillus thuringiensis in caterpillars and associated materials collected from protected tropical forests in northwestern Costa Rica |
title_short |
Bacillus thuringiensis in caterpillars and associated materials collected from protected tropical forests in northwestern Costa Rica |
title_full |
Bacillus thuringiensis in caterpillars and associated materials collected from protected tropical forests in northwestern Costa Rica |
title_fullStr |
Bacillus thuringiensis in caterpillars and associated materials collected from protected tropical forests in northwestern Costa Rica |
title_full_unstemmed |
Bacillus thuringiensis in caterpillars and associated materials collected from protected tropical forests in northwestern Costa Rica |
title_sort |
bacillus thuringiensis in caterpillars and associated materials collected from protected tropical forests in northwestern costa rica |
publisher |
Vicerractoría Investigación |
series |
Revista de Biología Tropical |
issn |
0034-7744 2215-2075 |
publishDate |
2006-06-01 |
description |
Bacillus thuringiensis (Bt) synthesizes crystalline inclusions that are toxic to caterpillars (Lepidoptera) and other orders of invertebrates. Materials associated with 37 caterpillars from 16 species, collected while feeding on 15 different species of host plants in dry, cloud and rain forests located in the Área de Conservación Guanacaste in northwestern Costa Rica, were examined for the presence of Bt. From a total of 101 derived samples, 25 Bt isolates were cultured: 56% from host plant leaves, 8% from caterpillar guts and 36% from caterpillar fecal pellets. Bt was isolated from at least one sample in 38% of the systems constituted by the food plant, gut and fecal pellets corresponding to a single caterpillar. Four different morphologies of crystalline inclusions were observed, with bipyramidal and irregular crystal morphologies being the most prevalent. Rev. Biol. Trop. 54(2): 265-271. Epub 2006 Jun 01.<br>Bacillus thuringiensis (Bt) sintetiza inclusiones cristalinas que resultan tóxicas para algunas larvas de lepidópteros y otros órdenes de invertebrados. Su presencia fue examinada en materiales asociados a 37 orugas de mariposas de 16 especies, las cuales fueron colectadas mientras se alimentaban en 15 especies diferentes de plantas hospederas en bosques secos, nubosos y húmedos localizados dentro del Área de Conservación Guanacaste (ACG) en el noroeste de Costa Rica. A partir de un total de 101 muestras se obtuvo 25 aislamientos de Bt: 56% a partir de material foliar de las plantas hospederas, 8% a partir del contenido intestinal de las larvas y 36% a partir de sus excrementos. Esta bacteria fue cultivada a partir de al menos uno de los 3 diferentes tipos de muestra asociados a una oruga particular (planta hospedera, intestino, excremento) en 38% de los casos posibles. En la colección de aislamientos obtenida se observaron cuatro morfologías de inclusiones cristalinas, siendo aquellas bipiramidales e irregulares las más prevalentes. |
topic |
Bacillus thuringiensis larvas de mariposa plantas hospederas tractos intestinales de insectos material fecal áreas protegidas Bacillus thuringiensis caterpillars food plants insect guts fecal pellets conserved wildlands |
url |
http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442006000200004 |
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