In vitro and in vivo assessment of the proresolutive and antiresorptive actions of resolvin D1: relevance to arthritis
Abstract Background Resolvin D1 (RvD1), an important member of resolvins, exerts a wide spectrum of biological effects, including resolution of inflammation, tissue repair, and preservation of cell viability. The aim of the present study is to investigate the anti-arthritic potential and clarify the...
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doaj-308d8661d79b4fadb475d107a9cf84dc2020-11-25T02:57:58ZengBMCArthritis Research & Therapy1478-63622019-03-0121111410.1186/s13075-019-1852-8In vitro and in vivo assessment of the proresolutive and antiresorptive actions of resolvin D1: relevance to arthritisHouda Abir Benabdoun0Merve Kulbay1Elsa-Patricia Rondon2Francis Vallières3Qin Shi4Julio Fernandes5Hassan Fahmi6Mohamed Benderdour7Department of Pharmacology, Université de MontréalOrthopedic Research Laboratory, Hôpital du Sacré-Cœur de MontréalOrthopedic Research Laboratory, Hôpital du Sacré-Cœur de MontréalOrthopedic Research Laboratory, Hôpital du Sacré-Cœur de MontréalOrthopedic Research Laboratory, Hôpital du Sacré-Cœur de MontréalOrthopedic Research Laboratory, Hôpital du Sacré-Cœur de MontréalOsteoarthritis Research Unit, Centre Hospitalier de l’Université de MontréalOrthopedic Research Laboratory, Hôpital du Sacré-Cœur de MontréalAbstract Background Resolvin D1 (RvD1), an important member of resolvins, exerts a wide spectrum of biological effects, including resolution of inflammation, tissue repair, and preservation of cell viability. The aim of the present study is to investigate the anti-arthritic potential and clarify the bone protective actions of RvD1 in vitro and in vivo. Methods RAW264.7 cells were treated with 50 ng/ml LPS for 72 h in the presence or absence of RvD1 (0–500 nM). Primary human monocytes were treated with M-CSF + RANKL for 14 days ± RvD1 (0–500 nM) with or without siRNA against RvD1 receptor FPR2. Expressions of inflammatory mediators, degrading enzymes, osteoclasts (OC) formation, and bone resorption were analyzed. The therapeutic effect of RvD1 (0–1000 ng) was carried out in murine collagen antibody-induced arthritis. Arthritis scoring, joint histology, and inflammatory and bone turnover markers were measured. Results RvD1 is not toxic and inhibits OC differentiation and activation. It decreases bone resorption, as assessed by the inhibition of TRAP and cathepsin K expression, hydroxyapatite matrix resorption, and bone loss. In addition, RvD1 reduces TNF-α, IL-1β, IFN-γ, PGE2, and RANK and concurrently enhances IL-10 in OC. Moreover, in arthritic mice, RvD1 alleviates clinical score, paw inflammation, and bone and joint destructions. Besides, RvD1 reduces inflammatory mediators and markedly decreases serum markers of bone and cartilage turnover. Conclusion Our results provide additional evidence that RvD1 plays a key role in preventing bone resorption and other pathophysiological changes associated with arthritis. The study highlights the clinical relevance of RvD1 as a potential compound for the treatment of inflammatory arthritis and related bone disorders.http://link.springer.com/article/10.1186/s13075-019-1852-8ArthritisInflammationResolvin D1Bone resorptionMice |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Houda Abir Benabdoun Merve Kulbay Elsa-Patricia Rondon Francis Vallières Qin Shi Julio Fernandes Hassan Fahmi Mohamed Benderdour |
spellingShingle |
Houda Abir Benabdoun Merve Kulbay Elsa-Patricia Rondon Francis Vallières Qin Shi Julio Fernandes Hassan Fahmi Mohamed Benderdour In vitro and in vivo assessment of the proresolutive and antiresorptive actions of resolvin D1: relevance to arthritis Arthritis Research & Therapy Arthritis Inflammation Resolvin D1 Bone resorption Mice |
author_facet |
Houda Abir Benabdoun Merve Kulbay Elsa-Patricia Rondon Francis Vallières Qin Shi Julio Fernandes Hassan Fahmi Mohamed Benderdour |
author_sort |
Houda Abir Benabdoun |
title |
In vitro and in vivo assessment of the proresolutive and antiresorptive actions of resolvin D1: relevance to arthritis |
title_short |
In vitro and in vivo assessment of the proresolutive and antiresorptive actions of resolvin D1: relevance to arthritis |
title_full |
In vitro and in vivo assessment of the proresolutive and antiresorptive actions of resolvin D1: relevance to arthritis |
title_fullStr |
In vitro and in vivo assessment of the proresolutive and antiresorptive actions of resolvin D1: relevance to arthritis |
title_full_unstemmed |
In vitro and in vivo assessment of the proresolutive and antiresorptive actions of resolvin D1: relevance to arthritis |
title_sort |
in vitro and in vivo assessment of the proresolutive and antiresorptive actions of resolvin d1: relevance to arthritis |
publisher |
BMC |
series |
Arthritis Research & Therapy |
issn |
1478-6362 |
publishDate |
2019-03-01 |
description |
Abstract Background Resolvin D1 (RvD1), an important member of resolvins, exerts a wide spectrum of biological effects, including resolution of inflammation, tissue repair, and preservation of cell viability. The aim of the present study is to investigate the anti-arthritic potential and clarify the bone protective actions of RvD1 in vitro and in vivo. Methods RAW264.7 cells were treated with 50 ng/ml LPS for 72 h in the presence or absence of RvD1 (0–500 nM). Primary human monocytes were treated with M-CSF + RANKL for 14 days ± RvD1 (0–500 nM) with or without siRNA against RvD1 receptor FPR2. Expressions of inflammatory mediators, degrading enzymes, osteoclasts (OC) formation, and bone resorption were analyzed. The therapeutic effect of RvD1 (0–1000 ng) was carried out in murine collagen antibody-induced arthritis. Arthritis scoring, joint histology, and inflammatory and bone turnover markers were measured. Results RvD1 is not toxic and inhibits OC differentiation and activation. It decreases bone resorption, as assessed by the inhibition of TRAP and cathepsin K expression, hydroxyapatite matrix resorption, and bone loss. In addition, RvD1 reduces TNF-α, IL-1β, IFN-γ, PGE2, and RANK and concurrently enhances IL-10 in OC. Moreover, in arthritic mice, RvD1 alleviates clinical score, paw inflammation, and bone and joint destructions. Besides, RvD1 reduces inflammatory mediators and markedly decreases serum markers of bone and cartilage turnover. Conclusion Our results provide additional evidence that RvD1 plays a key role in preventing bone resorption and other pathophysiological changes associated with arthritis. The study highlights the clinical relevance of RvD1 as a potential compound for the treatment of inflammatory arthritis and related bone disorders. |
topic |
Arthritis Inflammation Resolvin D1 Bone resorption Mice |
url |
http://link.springer.com/article/10.1186/s13075-019-1852-8 |
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