N-acetylcysteine inhibit biofilms produced by <it>Pseudomonas aeruginosa</it>
<p>Abstract</p> <p>Background</p> <p><it>Pseudomonas aeruginosa </it>is a common pathogen in chronic respiratory tract infections. It typically makes a biofilm, which makes treatment of these infections difficult. In this study, we investigated the inhibitor...
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doaj-30a3348bf5234108b39b12d48cdeac992020-11-24T22:30:23ZengBMCBMC Microbiology1471-21802010-05-0110114010.1186/1471-2180-10-140N-acetylcysteine inhibit biofilms produced by <it>Pseudomonas aeruginosa</it>Liu YouningZhao Tiemei<p>Abstract</p> <p>Background</p> <p><it>Pseudomonas aeruginosa </it>is a common pathogen in chronic respiratory tract infections. It typically makes a biofilm, which makes treatment of these infections difficult. In this study, we investigated the inhibitory effects of N-acetylcysteine (NAC) on biofilms produced by <it>P. aeruginosa</it>.</p> <p>Results</p> <p>We found that minimum inhibitory concentrations (MICs) of NAC for most isolates of <it>P. aeruginosa </it>were 10 to 40 mg/ml, the combination of NAC and ciprofloxacin (CIP) demonstrated either synergy (50%) or no interaction (50%) against the <it>P. aeruginosa </it>strains. NAC at 0.5 mg/ml could detach mature <it>P. aeruginosa </it>biofilms. Disruption was proportional to NAC concentrations, and biofilms were completely disrupted at 10 mg/ml NAC. Analysis using COMSTAT software also showed that PAO1 biofilm biomass decreased and its heterogeneity increased as NAC concentration increased. NAC and ciprofloxacin showed significant killing of <it>P. aeruginosa </it>in biofilms at 2.5 mg/ml and > 2 MIC, respectively (<it>p </it>< 0.01). NAC-ciprofloxacin combinations consistently decreased viable biofilm-associated bacteria relative to the control; this combination was synergistic at NAC of 0.5 mg/ml and CIP at 1/2MIC (<it>p</it> < 0.01). Extracellular polysaccharides (EPS) production by <it>P. aeruginosa </it>also decreased by 27.64% and 44.59% at NAC concentrations of 0.5 mg/ml and 1 mg/ml.</p> <p>Conclusions</p> <p>NAC has anti-bacterial properties against <it>P. aeruginosa </it>and may detach <it>P. aeruginosa </it>biofilms. Use of NAC may be a new strategy for the treatment of biofilm-associated chronic respiratory infections due to <it>P. aeruginosa</it>, although it would be appropriate to conduct clinical studies to confirm this.</p> http://www.biomedcentral.com/1471-2180/10/140 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Liu Youning Zhao Tiemei |
spellingShingle |
Liu Youning Zhao Tiemei N-acetylcysteine inhibit biofilms produced by <it>Pseudomonas aeruginosa</it> BMC Microbiology |
author_facet |
Liu Youning Zhao Tiemei |
author_sort |
Liu Youning |
title |
N-acetylcysteine inhibit biofilms produced by <it>Pseudomonas aeruginosa</it> |
title_short |
N-acetylcysteine inhibit biofilms produced by <it>Pseudomonas aeruginosa</it> |
title_full |
N-acetylcysteine inhibit biofilms produced by <it>Pseudomonas aeruginosa</it> |
title_fullStr |
N-acetylcysteine inhibit biofilms produced by <it>Pseudomonas aeruginosa</it> |
title_full_unstemmed |
N-acetylcysteine inhibit biofilms produced by <it>Pseudomonas aeruginosa</it> |
title_sort |
n-acetylcysteine inhibit biofilms produced by <it>pseudomonas aeruginosa</it> |
publisher |
BMC |
series |
BMC Microbiology |
issn |
1471-2180 |
publishDate |
2010-05-01 |
description |
<p>Abstract</p> <p>Background</p> <p><it>Pseudomonas aeruginosa </it>is a common pathogen in chronic respiratory tract infections. It typically makes a biofilm, which makes treatment of these infections difficult. In this study, we investigated the inhibitory effects of N-acetylcysteine (NAC) on biofilms produced by <it>P. aeruginosa</it>.</p> <p>Results</p> <p>We found that minimum inhibitory concentrations (MICs) of NAC for most isolates of <it>P. aeruginosa </it>were 10 to 40 mg/ml, the combination of NAC and ciprofloxacin (CIP) demonstrated either synergy (50%) or no interaction (50%) against the <it>P. aeruginosa </it>strains. NAC at 0.5 mg/ml could detach mature <it>P. aeruginosa </it>biofilms. Disruption was proportional to NAC concentrations, and biofilms were completely disrupted at 10 mg/ml NAC. Analysis using COMSTAT software also showed that PAO1 biofilm biomass decreased and its heterogeneity increased as NAC concentration increased. NAC and ciprofloxacin showed significant killing of <it>P. aeruginosa </it>in biofilms at 2.5 mg/ml and > 2 MIC, respectively (<it>p </it>< 0.01). NAC-ciprofloxacin combinations consistently decreased viable biofilm-associated bacteria relative to the control; this combination was synergistic at NAC of 0.5 mg/ml and CIP at 1/2MIC (<it>p</it> < 0.01). Extracellular polysaccharides (EPS) production by <it>P. aeruginosa </it>also decreased by 27.64% and 44.59% at NAC concentrations of 0.5 mg/ml and 1 mg/ml.</p> <p>Conclusions</p> <p>NAC has anti-bacterial properties against <it>P. aeruginosa </it>and may detach <it>P. aeruginosa </it>biofilms. Use of NAC may be a new strategy for the treatment of biofilm-associated chronic respiratory infections due to <it>P. aeruginosa</it>, although it would be appropriate to conduct clinical studies to confirm this.</p> |
url |
http://www.biomedcentral.com/1471-2180/10/140 |
work_keys_str_mv |
AT liuyouning nacetylcysteineinhibitbiofilmsproducedbyitpseudomonasaeruginosait AT zhaotiemei nacetylcysteineinhibitbiofilmsproducedbyitpseudomonasaeruginosait |
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