OPTIMIZATION OF MEDIUM COMPOSITIONS FOR SACCHAROMYCES BOULARDII BY BOX-BEHNKEN DESIGN
Based on the previous experimental results, the Box-Behnken design (BBD) was employed to study the individual and interactive effects of sucrose, malt extract, calf serum and sodium citrate on the growth of Saccharomyces boulardii, and the optimum medium compositions were obtained. Meanwhile, the op...
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Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
2016-12-01
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doaj-30bbe6d6bb4f48f98e3a157d781cc8b02020-11-24T22:45:10ZengAlma Mater Publishing House "Vasile Alecsandri" University of BacauScientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry1582-540X1582-540X2016-12-01174405416OPTIMIZATION OF MEDIUM COMPOSITIONS FOR SACCHAROMYCES BOULARDII BY BOX-BEHNKEN DESIGNZHANGTENG LEI0HE CHEN1DAN HUANG2YALING ZHAI3GUOWEI SHU4Shaanxi University of Science & Technology, School of Food and Biological Engineering, 710021, Xi’an, ChinaShaanxi University of Science & Technology, School of Food and Biological Engineering, 710021, Xi’an, ChinaShaanxi Pucheng Shiyang Feed Co., Ltd., 715501, Xi’an, China Shaanxi Pucheng Shiyang Feed Co., Ltd., 715501, Xi’an, China Shaanxi University of Science & Technology, School of Food and Biological Engineering, 710021, Xi’an, ChinaBased on the previous experimental results, the Box-Behnken design (BBD) was employed to study the individual and interactive effects of sucrose, malt extract, calf serum and sodium citrate on the growth of Saccharomyces boulardii, and the optimum medium compositions were obtained. Meanwhile, the optical density (OD) in the fermentation suspension was measured at 560 nm after 36 h of incubation. It is shown that the optimized medium compositions were 36.28 g·L-1 sucrose, 6.38 g·L-1 malt extract, 5.69 g·L-1 calf serum and 5.3 g·L-1 sodium citrate. The result indicated that the growth of S. boulardii could increase significantly in the optimized medium, and the OD560nm value reached 1.397 ± 0.013 after 36 h, which increased 18.59 % compared with that of pre-optimized medium. In addition, the OD560nm value 1.397 ± 0.013 in the optimized medium was very closely to the expected value 1.394. This result suggested that optimization of medium compositions for S. boulardii by BBD in this study was reliable and effective.http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201604&vol=4&aid=4473Box-Behnken designcalf serummalt extractoptical densitySaccharomyces boulardiisodium citratesucrose |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
ZHANGTENG LEI HE CHEN DAN HUANG YALING ZHAI GUOWEI SHU |
spellingShingle |
ZHANGTENG LEI HE CHEN DAN HUANG YALING ZHAI GUOWEI SHU OPTIMIZATION OF MEDIUM COMPOSITIONS FOR SACCHAROMYCES BOULARDII BY BOX-BEHNKEN DESIGN Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry Box-Behnken design calf serum malt extract optical density Saccharomyces boulardii sodium citrate sucrose |
author_facet |
ZHANGTENG LEI HE CHEN DAN HUANG YALING ZHAI GUOWEI SHU |
author_sort |
ZHANGTENG LEI |
title |
OPTIMIZATION OF MEDIUM COMPOSITIONS FOR SACCHAROMYCES BOULARDII BY BOX-BEHNKEN DESIGN |
title_short |
OPTIMIZATION OF MEDIUM COMPOSITIONS FOR SACCHAROMYCES BOULARDII BY BOX-BEHNKEN DESIGN |
title_full |
OPTIMIZATION OF MEDIUM COMPOSITIONS FOR SACCHAROMYCES BOULARDII BY BOX-BEHNKEN DESIGN |
title_fullStr |
OPTIMIZATION OF MEDIUM COMPOSITIONS FOR SACCHAROMYCES BOULARDII BY BOX-BEHNKEN DESIGN |
title_full_unstemmed |
OPTIMIZATION OF MEDIUM COMPOSITIONS FOR SACCHAROMYCES BOULARDII BY BOX-BEHNKEN DESIGN |
title_sort |
optimization of medium compositions for saccharomyces boulardii by box-behnken design |
publisher |
Alma Mater Publishing House "Vasile Alecsandri" University of Bacau |
series |
Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
issn |
1582-540X 1582-540X |
publishDate |
2016-12-01 |
description |
Based on the previous experimental results, the Box-Behnken design (BBD) was employed to study the individual and interactive effects of sucrose, malt extract, calf serum and sodium citrate on the growth of Saccharomyces boulardii, and the optimum medium compositions were obtained. Meanwhile, the optical density (OD) in the fermentation suspension was measured at 560 nm after 36 h of incubation. It is shown that the optimized medium compositions were 36.28 g·L-1 sucrose, 6.38 g·L-1 malt extract, 5.69 g·L-1 calf serum and 5.3 g·L-1 sodium citrate. The result indicated that the growth of S. boulardii could increase significantly in the optimized medium, and the OD560nm value reached 1.397 ± 0.013 after 36 h, which increased 18.59 % compared with that of pre-optimized medium. In addition, the OD560nm value 1.397 ± 0.013 in the optimized medium was very closely to the expected value 1.394. This result suggested that optimization of medium compositions for S. boulardii by BBD in this study was reliable and effective. |
topic |
Box-Behnken design calf serum malt extract optical density Saccharomyces boulardii sodium citrate sucrose |
url |
http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201604&vol=4&aid=4473 |
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