SdiA Improves the Acid Tolerance of E. coli by Regulating GadW and GadY Expression
The acid tolerance mechanism is important for Escherichia coli to resist acidic conditions encountered in mammalian host digestive tract environment. Here, we explored how the LuxR protein SdiA influenced E. coli acid tolerance ability in the context of the glutamate- and glutamine-dependent acid re...
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doaj-30c6c8b03e3a4d65af461ac298006b5f2020-11-25T02:51:53ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-06-011110.3389/fmicb.2020.01078515125SdiA Improves the Acid Tolerance of E. coli by Regulating GadW and GadY ExpressionXingyan Ma0Xingyan Ma1Shebin Zhang2Shebin Zhang3Zhenjie Xu4Zhenjie Xu5Honglin Li6Honglin Li7Qian Xiao8Feng Qiu9Weizheng Zhang10Yifei Long11Dexiang Zheng12Bin Huang13Cha Chen14Yang Lu15Yang Lu16Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Laboratory Medicine, Nanhai Hospital, Southern Medical University, Foshan, ChinaDepartment of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaDepartment of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, ChinaThe acid tolerance mechanism is important for Escherichia coli to resist acidic conditions encountered in mammalian host digestive tract environment. Here, we explored how the LuxR protein SdiA influenced E. coli acid tolerance ability in the context of the glutamate- and glutamine-dependent acid resistance system (AR2). First, using a growth and acid shock assay under different acid stresses, we demonstrated that the deletion of sdiA in SM10λpir or BW25113 led to impaired growth under the acidic environment of pH 3–6, which was restored by complementary expression of SdiA. Next, transcriptome sequencing and qPCR disclosed that the expression of glutamate decarboxylase W (GadW) and GadY, the key members of the AR2 system, were regulated by SdiA. Further, β-galactosidase reporter assays showed that the promoter activity of gadW and gadY was positively regulated by SdiA. Moreover, qPCR and β-galactosidase reporter assays confirmed that the regulation of SdiA on GadW, but not GadY, could be enhanced by quorum sensing (QS) signal molecules AHLs. Collectively, these data suggest that SdiA plays a crucial role in acid tolerance regulation of E. coli. Our findings provide new insights into the important contribution of quorum sensing system AHLs–SdiA to the networks that regulate acid tolerance.https://www.frontiersin.org/article/10.3389/fmicb.2020.01078/fullSdiAglutamate decarboxylase W (GadW)glutamate decarboxylase Y (GadY)acid toleranceEscherichia coli |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xingyan Ma Xingyan Ma Shebin Zhang Shebin Zhang Zhenjie Xu Zhenjie Xu Honglin Li Honglin Li Qian Xiao Feng Qiu Weizheng Zhang Yifei Long Dexiang Zheng Bin Huang Cha Chen Yang Lu Yang Lu |
spellingShingle |
Xingyan Ma Xingyan Ma Shebin Zhang Shebin Zhang Zhenjie Xu Zhenjie Xu Honglin Li Honglin Li Qian Xiao Feng Qiu Weizheng Zhang Yifei Long Dexiang Zheng Bin Huang Cha Chen Yang Lu Yang Lu SdiA Improves the Acid Tolerance of E. coli by Regulating GadW and GadY Expression Frontiers in Microbiology SdiA glutamate decarboxylase W (GadW) glutamate decarboxylase Y (GadY) acid tolerance Escherichia coli |
author_facet |
Xingyan Ma Xingyan Ma Shebin Zhang Shebin Zhang Zhenjie Xu Zhenjie Xu Honglin Li Honglin Li Qian Xiao Feng Qiu Weizheng Zhang Yifei Long Dexiang Zheng Bin Huang Cha Chen Yang Lu Yang Lu |
author_sort |
Xingyan Ma |
title |
SdiA Improves the Acid Tolerance of E. coli by Regulating GadW and GadY Expression |
title_short |
SdiA Improves the Acid Tolerance of E. coli by Regulating GadW and GadY Expression |
title_full |
SdiA Improves the Acid Tolerance of E. coli by Regulating GadW and GadY Expression |
title_fullStr |
SdiA Improves the Acid Tolerance of E. coli by Regulating GadW and GadY Expression |
title_full_unstemmed |
SdiA Improves the Acid Tolerance of E. coli by Regulating GadW and GadY Expression |
title_sort |
sdia improves the acid tolerance of e. coli by regulating gadw and gady expression |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2020-06-01 |
description |
The acid tolerance mechanism is important for Escherichia coli to resist acidic conditions encountered in mammalian host digestive tract environment. Here, we explored how the LuxR protein SdiA influenced E. coli acid tolerance ability in the context of the glutamate- and glutamine-dependent acid resistance system (AR2). First, using a growth and acid shock assay under different acid stresses, we demonstrated that the deletion of sdiA in SM10λpir or BW25113 led to impaired growth under the acidic environment of pH 3–6, which was restored by complementary expression of SdiA. Next, transcriptome sequencing and qPCR disclosed that the expression of glutamate decarboxylase W (GadW) and GadY, the key members of the AR2 system, were regulated by SdiA. Further, β-galactosidase reporter assays showed that the promoter activity of gadW and gadY was positively regulated by SdiA. Moreover, qPCR and β-galactosidase reporter assays confirmed that the regulation of SdiA on GadW, but not GadY, could be enhanced by quorum sensing (QS) signal molecules AHLs. Collectively, these data suggest that SdiA plays a crucial role in acid tolerance regulation of E. coli. Our findings provide new insights into the important contribution of quorum sensing system AHLs–SdiA to the networks that regulate acid tolerance. |
topic |
SdiA glutamate decarboxylase W (GadW) glutamate decarboxylase Y (GadY) acid tolerance Escherichia coli |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2020.01078/full |
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