Evaluation of the Impact of Imprinted Polymer Particles on Morphology and Motility of Breast Cancer Cells by Using Digital Holographic Cytometry
Breast cancer is the second most common cancer type worldwide and breast cancer metastasis accounts for the majority of breast cancer-related deaths. Tumour cells produce increased levels of sialic acid (SA) that terminates the monosaccharide on glycan chains of the glycosylated proteins. SA can con...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-01-01
|
Series: | Applied Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-3417/10/3/750 |
id |
doaj-30e10ca08fdf4ca9918daf8aee5a33bb |
---|---|
record_format |
Article |
spelling |
doaj-30e10ca08fdf4ca9918daf8aee5a33bb2020-11-25T01:30:14ZengMDPI AGApplied Sciences2076-34172020-01-0110375010.3390/app10030750app10030750Evaluation of the Impact of Imprinted Polymer Particles on Morphology and Motility of Breast Cancer Cells by Using Digital Holographic CytometryMegha Patel0Marek Feith1Birgit Janicke2Kersti Alm3Zahra El-Schich4Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, 205 06 Malmö, SwedenDepartment of Physiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech RepublicPhase Holographic Imaging AB, 223 63 Lund, SwedenPhase Holographic Imaging AB, 223 63 Lund, SwedenDepartment of Biomedical Sciences, Faculty of Health and Society, Malmö University, 205 06 Malmö, SwedenBreast cancer is the second most common cancer type worldwide and breast cancer metastasis accounts for the majority of breast cancer-related deaths. Tumour cells produce increased levels of sialic acid (SA) that terminates the monosaccharide on glycan chains of the glycosylated proteins. SA can contribute to cellular recognition, cancer invasiveness and increase the metastatic potential of cancer cells. SA-templated molecularly imprinted polymers (MIPs) have been proposed as promising reporters for specific targeting of cancer cells when deployed in nanoparticle format. The sialic acid-molecularly imprinted polymers (SA-MIPs), which use SA for the generation of binding sites through which the nanoparticles can target and stain breast cancer cells, opens new strategies for efficient diagnostic tools. This study aims at monitoring the effects of SA-MIPs on morphology and motility of the epithelial type MCF-7 and the highly metastatic MDAMB231 breast cancer cell lines, using digital holographic cytometry (DHC). DHC is a label-free technique that is used in cell morphology studies of e.g., cell volume, area and thickness as well as in motility studies. Here, we show that MCF-7 cells move slower than MDAMB231 cells. We also show that SA-MIPs have an effect on cell morphology, motility and viability of both cell lines. In conclusion, by using DH microscopy, we could detect SA-MIPs impact on different breast cancer cells regarding morphology and motility.https://www.mdpi.com/2076-3417/10/3/750breast cancerdigital holographic cytometrymolecularly imprinted polymersmotilitysialic acidviability |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Megha Patel Marek Feith Birgit Janicke Kersti Alm Zahra El-Schich |
spellingShingle |
Megha Patel Marek Feith Birgit Janicke Kersti Alm Zahra El-Schich Evaluation of the Impact of Imprinted Polymer Particles on Morphology and Motility of Breast Cancer Cells by Using Digital Holographic Cytometry Applied Sciences breast cancer digital holographic cytometry molecularly imprinted polymers motility sialic acid viability |
author_facet |
Megha Patel Marek Feith Birgit Janicke Kersti Alm Zahra El-Schich |
author_sort |
Megha Patel |
title |
Evaluation of the Impact of Imprinted Polymer Particles on Morphology and Motility of Breast Cancer Cells by Using Digital Holographic Cytometry |
title_short |
Evaluation of the Impact of Imprinted Polymer Particles on Morphology and Motility of Breast Cancer Cells by Using Digital Holographic Cytometry |
title_full |
Evaluation of the Impact of Imprinted Polymer Particles on Morphology and Motility of Breast Cancer Cells by Using Digital Holographic Cytometry |
title_fullStr |
Evaluation of the Impact of Imprinted Polymer Particles on Morphology and Motility of Breast Cancer Cells by Using Digital Holographic Cytometry |
title_full_unstemmed |
Evaluation of the Impact of Imprinted Polymer Particles on Morphology and Motility of Breast Cancer Cells by Using Digital Holographic Cytometry |
title_sort |
evaluation of the impact of imprinted polymer particles on morphology and motility of breast cancer cells by using digital holographic cytometry |
publisher |
MDPI AG |
series |
Applied Sciences |
issn |
2076-3417 |
publishDate |
2020-01-01 |
description |
Breast cancer is the second most common cancer type worldwide and breast cancer metastasis accounts for the majority of breast cancer-related deaths. Tumour cells produce increased levels of sialic acid (SA) that terminates the monosaccharide on glycan chains of the glycosylated proteins. SA can contribute to cellular recognition, cancer invasiveness and increase the metastatic potential of cancer cells. SA-templated molecularly imprinted polymers (MIPs) have been proposed as promising reporters for specific targeting of cancer cells when deployed in nanoparticle format. The sialic acid-molecularly imprinted polymers (SA-MIPs), which use SA for the generation of binding sites through which the nanoparticles can target and stain breast cancer cells, opens new strategies for efficient diagnostic tools. This study aims at monitoring the effects of SA-MIPs on morphology and motility of the epithelial type MCF-7 and the highly metastatic MDAMB231 breast cancer cell lines, using digital holographic cytometry (DHC). DHC is a label-free technique that is used in cell morphology studies of e.g., cell volume, area and thickness as well as in motility studies. Here, we show that MCF-7 cells move slower than MDAMB231 cells. We also show that SA-MIPs have an effect on cell morphology, motility and viability of both cell lines. In conclusion, by using DH microscopy, we could detect SA-MIPs impact on different breast cancer cells regarding morphology and motility. |
topic |
breast cancer digital holographic cytometry molecularly imprinted polymers motility sialic acid viability |
url |
https://www.mdpi.com/2076-3417/10/3/750 |
work_keys_str_mv |
AT meghapatel evaluationoftheimpactofimprintedpolymerparticlesonmorphologyandmotilityofbreastcancercellsbyusingdigitalholographiccytometry AT marekfeith evaluationoftheimpactofimprintedpolymerparticlesonmorphologyandmotilityofbreastcancercellsbyusingdigitalholographiccytometry AT birgitjanicke evaluationoftheimpactofimprintedpolymerparticlesonmorphologyandmotilityofbreastcancercellsbyusingdigitalholographiccytometry AT kerstialm evaluationoftheimpactofimprintedpolymerparticlesonmorphologyandmotilityofbreastcancercellsbyusingdigitalholographiccytometry AT zahraelschich evaluationoftheimpactofimprintedpolymerparticlesonmorphologyandmotilityofbreastcancercellsbyusingdigitalholographiccytometry |
_version_ |
1725092754862637056 |