Outbreak of KPC-2 Carbapenem-resistant Klebsiella pneumoniae ST76 and Carbapenem-resistant K2 Hypervirulent Klebsiella pneumoniae ST375 strains in Northeast China: molecular and virulent characteristics
Abstract Background Carbapenem-resistant hypervirulent Klebsiella pneumoniae strains have recently come into existence worldwide; however, researchers in northeast China are not aware of their clinical features and molecular characteristics. Methods Here, the molecular and virulent characteristics o...
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doaj-30e8784706444d708c44d124669ae34f2020-11-25T03:59:05ZengBMCBMC Infectious Diseases1471-23342020-07-0120111410.1186/s12879-020-05143-yOutbreak of KPC-2 Carbapenem-resistant Klebsiella pneumoniae ST76 and Carbapenem-resistant K2 Hypervirulent Klebsiella pneumoniae ST375 strains in Northeast China: molecular and virulent characteristicsShanshan Su0Jisheng Zhang1Yongxin Zhao2Lan Yu3Yong Wang4Yuchao Wang5Mingjia Bao6Yu Fu7Chunjiang Li8Xiaoli Zhang9Yongchuan hospital of Chongqing Medical UniversityYongchuan hospital of Chongqing Medical UniversityFirst Affiliated Hospital of Jiamusi UniversityFirst Affiliated Hospital of Jiamusi UniversityFirst Affiliated Hospital of Jiamusi UniversityFirst Affiliated Hospital of Jiamusi UniversityCenter for Disease Control and PreventionCenter for Disease Control and PreventionFirst Affiliated Hospital of Jiamusi UniversityYongchuan hospital of Chongqing Medical UniversityAbstract Background Carbapenem-resistant hypervirulent Klebsiella pneumoniae strains have recently come into existence worldwide; however, researchers in northeast China are not aware of their clinical features and molecular characteristics. Methods Here, the molecular and virulent characteristics of 44 carbapenem-resistant K. pneumoniae (CRKP) isolates collected from January 2015 to December 2017 were studied. Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were carried out to define the clonal relatedness among the isolates. PCR and capsular serotyping of the virulence-associated genes, as well as biofilm formation and serum complement-mediated killing assays, were employed to determine the virulent potential. The genomic features and associated mobile genetic elements of JmsCRE57 were detected by whole genome sequencing. Results The only positive isolate was JmsCRE57, which belonged to the ST375 serotype K2 that expressed uge, mrkD, fimH, kpn, aerobactin and rmpA virulence-associated genes and showed strong biofilm formation and serum sensitivity. Sequencing results showed that the JmsCRE57 genome mainly consisted of a circular chromosome, three antimicrobial resistant plasmids and a virulent plasmid. The antimicrobial resistant plasmid expressing bla KPC-2 , bla CTX-M-15 , aph(3″)-Ib, aph(6)-Id, qnrB1, aac(3)-IIa, aac(6′)-Ib-cr, bla OXA-1 , bla TEM-1B , catB4, sul2, dfrA14 and bla SHV-99 . The virulent plasmid belonged to the IncHI1B group, which is mainly composed of mucoid phenotype genes and siderophore-associated genes. The remaining CRKP strains that expressed uge, fimH, mrkD and kpn virulence-associated genes were not successfully typed. Conclusion Our results provide new insights on the epidemiology of carbapenem-resistant K2 hypervirulent K. pneumoniae ST375 and CRKP ST76 strains in northeast China, which may help control their future outbreaks.http://link.springer.com/article/10.1186/s12879-020-05143-yCarbapenem resistanceKlebsiella pneumoniaeVirulenceEpidemiologyWhole-genome sequencing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shanshan Su Jisheng Zhang Yongxin Zhao Lan Yu Yong Wang Yuchao Wang Mingjia Bao Yu Fu Chunjiang Li Xiaoli Zhang |
spellingShingle |
Shanshan Su Jisheng Zhang Yongxin Zhao Lan Yu Yong Wang Yuchao Wang Mingjia Bao Yu Fu Chunjiang Li Xiaoli Zhang Outbreak of KPC-2 Carbapenem-resistant Klebsiella pneumoniae ST76 and Carbapenem-resistant K2 Hypervirulent Klebsiella pneumoniae ST375 strains in Northeast China: molecular and virulent characteristics BMC Infectious Diseases Carbapenem resistance Klebsiella pneumoniae Virulence Epidemiology Whole-genome sequencing |
author_facet |
Shanshan Su Jisheng Zhang Yongxin Zhao Lan Yu Yong Wang Yuchao Wang Mingjia Bao Yu Fu Chunjiang Li Xiaoli Zhang |
author_sort |
Shanshan Su |
title |
Outbreak of KPC-2 Carbapenem-resistant Klebsiella pneumoniae ST76 and Carbapenem-resistant K2 Hypervirulent Klebsiella pneumoniae ST375 strains in Northeast China: molecular and virulent characteristics |
title_short |
Outbreak of KPC-2 Carbapenem-resistant Klebsiella pneumoniae ST76 and Carbapenem-resistant K2 Hypervirulent Klebsiella pneumoniae ST375 strains in Northeast China: molecular and virulent characteristics |
title_full |
Outbreak of KPC-2 Carbapenem-resistant Klebsiella pneumoniae ST76 and Carbapenem-resistant K2 Hypervirulent Klebsiella pneumoniae ST375 strains in Northeast China: molecular and virulent characteristics |
title_fullStr |
Outbreak of KPC-2 Carbapenem-resistant Klebsiella pneumoniae ST76 and Carbapenem-resistant K2 Hypervirulent Klebsiella pneumoniae ST375 strains in Northeast China: molecular and virulent characteristics |
title_full_unstemmed |
Outbreak of KPC-2 Carbapenem-resistant Klebsiella pneumoniae ST76 and Carbapenem-resistant K2 Hypervirulent Klebsiella pneumoniae ST375 strains in Northeast China: molecular and virulent characteristics |
title_sort |
outbreak of kpc-2 carbapenem-resistant klebsiella pneumoniae st76 and carbapenem-resistant k2 hypervirulent klebsiella pneumoniae st375 strains in northeast china: molecular and virulent characteristics |
publisher |
BMC |
series |
BMC Infectious Diseases |
issn |
1471-2334 |
publishDate |
2020-07-01 |
description |
Abstract Background Carbapenem-resistant hypervirulent Klebsiella pneumoniae strains have recently come into existence worldwide; however, researchers in northeast China are not aware of their clinical features and molecular characteristics. Methods Here, the molecular and virulent characteristics of 44 carbapenem-resistant K. pneumoniae (CRKP) isolates collected from January 2015 to December 2017 were studied. Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were carried out to define the clonal relatedness among the isolates. PCR and capsular serotyping of the virulence-associated genes, as well as biofilm formation and serum complement-mediated killing assays, were employed to determine the virulent potential. The genomic features and associated mobile genetic elements of JmsCRE57 were detected by whole genome sequencing. Results The only positive isolate was JmsCRE57, which belonged to the ST375 serotype K2 that expressed uge, mrkD, fimH, kpn, aerobactin and rmpA virulence-associated genes and showed strong biofilm formation and serum sensitivity. Sequencing results showed that the JmsCRE57 genome mainly consisted of a circular chromosome, three antimicrobial resistant plasmids and a virulent plasmid. The antimicrobial resistant plasmid expressing bla KPC-2 , bla CTX-M-15 , aph(3″)-Ib, aph(6)-Id, qnrB1, aac(3)-IIa, aac(6′)-Ib-cr, bla OXA-1 , bla TEM-1B , catB4, sul2, dfrA14 and bla SHV-99 . The virulent plasmid belonged to the IncHI1B group, which is mainly composed of mucoid phenotype genes and siderophore-associated genes. The remaining CRKP strains that expressed uge, fimH, mrkD and kpn virulence-associated genes were not successfully typed. Conclusion Our results provide new insights on the epidemiology of carbapenem-resistant K2 hypervirulent K. pneumoniae ST375 and CRKP ST76 strains in northeast China, which may help control their future outbreaks. |
topic |
Carbapenem resistance Klebsiella pneumoniae Virulence Epidemiology Whole-genome sequencing |
url |
http://link.springer.com/article/10.1186/s12879-020-05143-y |
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