Assessment of co-segregated TLR4 genotypes among Nigerian children with asymptomatic and clinical malaria
Objective: To assess the occurrence and pattern of Toll-like receptor 4 (TLR4) co-segregated genotypes among children with Plasmodium falciparum malaria in Nigeria. Methods: In this case-control study, a total of 79 Plasmodium falciparum infected children aged 2–7 years and 105 age-matched uninfecte...
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Wolters Kluwer Medknow Publications
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doaj-30f965bc7faf4c49824c34676b7640ca2020-11-24T23:14:30ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912017-02-01729610210.1016/j.apjtb.2016.11.015Assessment of co-segregated TLR4 genotypes among Nigerian children with asymptomatic and clinical malariaBamidele Abiodun Iwalokun0Senapon Olusola Iwalokun1Bernice Enobong Udoh2Modinat Balogun3Molecular Biology and Biotechnology Division, Nigerian Institute of Medical Research, Lagos, NigeriaOlorunda Primary Health Centre, Lagos, NigeriaDepartment of Medical Microbiology and Parasitology, Olabisi Onabanjo University, Ogun State, NigeriaIru Primary Health Centre, Lagos, NigeriaObjective: To assess the occurrence and pattern of Toll-like receptor 4 (TLR4) co-segregated genotypes among children with Plasmodium falciparum malaria in Nigeria. Methods: In this case-control study, a total of 79 Plasmodium falciparum infected children aged 2–7 years and 105 age-matched uninfected controls of Yoruba descents in Lagos were studied. The extracted DNA samples were used for TLR4 genotyping at codons 299 (Asp > Gly) and 399 (Thr > Ile) by PCR-restriction fragment length polymorphism. Malaria infection was diagnosed by blood smear microscopy and infected children were stratified into asymptomatic, uncomplicated and severe malaria sub-groups. Malnutrition was determined by measuring the mid upper arm circumference and anemia was defined as hemoglobin < 11 g/dL. Results: The proportions of children with acute malnutrition and severe anemia were 12.0% and 3.2%, respectively. Parasitemia and malnutrition were not correlated and four distinct patterns of TLR4 genotypes were found in the study population: Asp299Asp/Thr399Thr (90.2%), Asp299Gly/Thr399Thr (4.3%), Gly299Gly/Thr399Thr (3.8%) and Asp299Gly/Thr399Ile (1.6%). These genotypes did not differ significantly (P > 0.05) in frequency between infected and non-infected children. However, low and high occurrences of the TLR4 Asp299Asp/Thr399Thr and Asp299Gly/Thr399Thr genotypes were observed in the severe malaria subgroup. Conclusions: This study reveals a protective role for TLR4 Asp299Gly/Thr399Ile and Asp299Asp/Thr399Thr genotypes against severe malaria in Nigerian children.http://www.sciencedirect.com/science/article/pii/S2221169116301824TLR4 polymorphismTLR4 co-segregated genotypesAsymptomatic malariaClinical malariaNigeria |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bamidele Abiodun Iwalokun Senapon Olusola Iwalokun Bernice Enobong Udoh Modinat Balogun |
spellingShingle |
Bamidele Abiodun Iwalokun Senapon Olusola Iwalokun Bernice Enobong Udoh Modinat Balogun Assessment of co-segregated TLR4 genotypes among Nigerian children with asymptomatic and clinical malaria Asian Pacific Journal of Tropical Biomedicine TLR4 polymorphism TLR4 co-segregated genotypes Asymptomatic malaria Clinical malaria Nigeria |
author_facet |
Bamidele Abiodun Iwalokun Senapon Olusola Iwalokun Bernice Enobong Udoh Modinat Balogun |
author_sort |
Bamidele Abiodun Iwalokun |
title |
Assessment of co-segregated TLR4 genotypes among Nigerian children with asymptomatic and clinical malaria |
title_short |
Assessment of co-segregated TLR4 genotypes among Nigerian children with asymptomatic and clinical malaria |
title_full |
Assessment of co-segregated TLR4 genotypes among Nigerian children with asymptomatic and clinical malaria |
title_fullStr |
Assessment of co-segregated TLR4 genotypes among Nigerian children with asymptomatic and clinical malaria |
title_full_unstemmed |
Assessment of co-segregated TLR4 genotypes among Nigerian children with asymptomatic and clinical malaria |
title_sort |
assessment of co-segregated tlr4 genotypes among nigerian children with asymptomatic and clinical malaria |
publisher |
Wolters Kluwer Medknow Publications |
series |
Asian Pacific Journal of Tropical Biomedicine |
issn |
2221-1691 |
publishDate |
2017-02-01 |
description |
Objective: To assess the occurrence and pattern of Toll-like receptor 4 (TLR4) co-segregated genotypes among children with Plasmodium falciparum malaria in Nigeria.
Methods: In this case-control study, a total of 79 Plasmodium falciparum infected children aged 2–7 years and 105 age-matched uninfected controls of Yoruba descents in Lagos were studied. The extracted DNA samples were used for TLR4 genotyping at codons 299 (Asp > Gly) and 399 (Thr > Ile) by PCR-restriction fragment length polymorphism. Malaria infection was diagnosed by blood smear microscopy and infected children were stratified into asymptomatic, uncomplicated and severe malaria sub-groups. Malnutrition was determined by measuring the mid upper arm circumference and anemia was defined as hemoglobin < 11 g/dL.
Results: The proportions of children with acute malnutrition and severe anemia were 12.0% and 3.2%, respectively. Parasitemia and malnutrition were not correlated and four distinct patterns of TLR4 genotypes were found in the study population: Asp299Asp/Thr399Thr (90.2%), Asp299Gly/Thr399Thr (4.3%), Gly299Gly/Thr399Thr (3.8%) and Asp299Gly/Thr399Ile (1.6%). These genotypes did not differ significantly (P > 0.05) in frequency between infected and non-infected children. However, low and high occurrences of the TLR4 Asp299Asp/Thr399Thr and Asp299Gly/Thr399Thr genotypes were observed in the severe malaria subgroup.
Conclusions: This study reveals a protective role for TLR4 Asp299Gly/Thr399Ile and Asp299Asp/Thr399Thr genotypes against severe malaria in Nigerian children. |
topic |
TLR4 polymorphism TLR4 co-segregated genotypes Asymptomatic malaria Clinical malaria Nigeria |
url |
http://www.sciencedirect.com/science/article/pii/S2221169116301824 |
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