Identification of a novel emaravirus infecting lilac through next-generation sequencing

Emaraviruses are multipartite negative-sense single-stranded viruses that usually infect flowering plants. Using unbiased next-generation sequencing (NGS) approach, a novel virus from lilac leaves with yellow mottle symptom was identified. Five viral genomic RNA segments were detected and completely...

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Main Authors:	Ya-qin WANG, Yu SONG, Meng-ji CAO, Qi CHENG, Jian-xiang WU, Tao HU
Format:	Article
Language:	English
Published:	Elsevier 2020-08-01
Series:	Journal of Integrative Agriculture
Subjects:	LiCRaV lilac Fimoviridae Emaravirus
Online Access:	http://www.sciencedirect.com/science/article/pii/S2095311919628066

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spelling	doaj-311b56fd0f9d4a7fa3683bda4993ec762021-06-08T04:41:57ZengElsevierJournal of Integrative Agriculture2095-31192020-08-0119820642071Identification of a novel emaravirus infecting lilac through next-generation sequencingYa-qin WANG0Yu SONG1Meng-ji CAO2Qi CHENG3Jian-xiang WU4Tao HU5State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R.ChinaState Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R.ChinaNational Citrus Engineering and Technology Research Center, Citrus Research Institute, Southwest University, Chongqing 400712, P.R.ChinaState Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R.ChinaState Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R.ChinaState Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China; Correspondence HU TaoEmaraviruses are multipartite negative-sense single-stranded viruses that usually infect flowering plants. Using unbiased next-generation sequencing (NGS) approach, a novel virus from lilac leaves with yellow mottle symptom was identified. Five viral genomic RNA segments were detected and completely sequenced. The molecular characteristics of the viral RNAs and proteins resemble those of members of the genus Emaravirus. The virus was found to be mechanically transmissible to Nicotiana benthamiana. Sequence alignment and phylogenetic analyses of the virus support the proposal of creating a new species in the genus Emaravirus for which the name lilac chlorotic ringspot-associated virus (LiCRaV) is proposed.http://www.sciencedirect.com/science/article/pii/S2095311919628066LiCRaVlilacFimoviridaeEmaravirus
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Ya-qin WANG Yu SONG Meng-ji CAO Qi CHENG Jian-xiang WU Tao HU
spellingShingle	Ya-qin WANG Yu SONG Meng-ji CAO Qi CHENG Jian-xiang WU Tao HU Identification of a novel emaravirus infecting lilac through next-generation sequencing Journal of Integrative Agriculture LiCRaV lilac Fimoviridae Emaravirus
author_facet	Ya-qin WANG Yu SONG Meng-ji CAO Qi CHENG Jian-xiang WU Tao HU
author_sort	Ya-qin WANG
title	Identification of a novel emaravirus infecting lilac through next-generation sequencing
title_short	Identification of a novel emaravirus infecting lilac through next-generation sequencing
title_full	Identification of a novel emaravirus infecting lilac through next-generation sequencing
title_fullStr	Identification of a novel emaravirus infecting lilac through next-generation sequencing
title_full_unstemmed	Identification of a novel emaravirus infecting lilac through next-generation sequencing
title_sort	identification of a novel emaravirus infecting lilac through next-generation sequencing
publisher	Elsevier
series	Journal of Integrative Agriculture
issn	2095-3119
publishDate	2020-08-01
description	Emaraviruses are multipartite negative-sense single-stranded viruses that usually infect flowering plants. Using unbiased next-generation sequencing (NGS) approach, a novel virus from lilac leaves with yellow mottle symptom was identified. Five viral genomic RNA segments were detected and completely sequenced. The molecular characteristics of the viral RNAs and proteins resemble those of members of the genus Emaravirus. The virus was found to be mechanically transmissible to Nicotiana benthamiana. Sequence alignment and phylogenetic analyses of the virus support the proposal of creating a new species in the genus Emaravirus for which the name lilac chlorotic ringspot-associated virus (LiCRaV) is proposed.
topic	LiCRaV lilac Fimoviridae Emaravirus
url	http://www.sciencedirect.com/science/article/pii/S2095311919628066
work_keys_str_mv	AT yaqinwang identificationofanovelemaravirusinfectinglilacthroughnextgenerationsequencing AT yusong identificationofanovelemaravirusinfectinglilacthroughnextgenerationsequencing AT mengjicao identificationofanovelemaravirusinfectinglilacthroughnextgenerationsequencing AT qicheng identificationofanovelemaravirusinfectinglilacthroughnextgenerationsequencing AT jianxiangwu identificationofanovelemaravirusinfectinglilacthroughnextgenerationsequencing AT taohu identificationofanovelemaravirusinfectinglilacthroughnextgenerationsequencing
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Identification of a novel emaravirus infecting lilac through next-generation sequencing

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