Phosphatidylethanol, ethyl glucuronide and ethanol in blood as complementary biomarkers for alcohol consumption
Alcohol biomarkers can monitor both recent and long-term drinking and provide information about drinking habits as a complement to self-reporting. Ethyl glucuronide (EtG) and phosphatidylethanol (PEth) are the most sensitive available biomarkers for this purpose. The present study aimed to collect d...
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doaj-312e15d23df640d78312cb2360066c982021-10-09T04:41:50ZengElsevierJournal of Mass Spectrometry and Advances in the Clinical Lab2667-145X2021-11-012237Phosphatidylethanol, ethyl glucuronide and ethanol in blood as complementary biomarkers for alcohol consumptionJasna Neumann0Olof Beck1Michael Böttcher2MVZ Medizinische Labore Dessau Kassel GmbH, GermanyMVZ Medizinische Labore Dessau Kassel GmbH, Germany; Department of Clinical Neuroscience, Karolinska Institute, SwedenMVZ Medizinische Labore Dessau Kassel GmbH, Germany; Corresponding author at: MVZ Medizinische Labore Dessau Kassel GmbH, Bauhüttenstrasse 6, D-06847 Dessau-Roßlau, Germany.Alcohol biomarkers can monitor both recent and long-term drinking and provide information about drinking habits as a complement to self-reporting. Ethyl glucuronide (EtG) and phosphatidylethanol (PEth) are the most sensitive available biomarkers for this purpose. The present study aimed to collect data on both PEth and EtG in the same blood sample, in addition to ethanol, in order to evaluate the combined use of these biomarkers. Venous EDTA blood samples (n = 1149) sent to the laboratory as part of a clinical routine service for measuring PEth were investigated. PEth and EtG concentrations were analyzed using liquid chromatography–mass spectrometry methods and ethanol with an enzymatic method. Of the 1149 samples, 95 were positive for ethanol (range 0.11–3.12 g/L), 454 for EtG (1.0–9739 ng/mL), 635 for PEth (0.014–6.0 µmol/L), 534 for PEth ≥ 0.050 µmol/L, and 315 for PEth ≥ 0.30 µmol/L. EtG and PEth concentrations seemed largely independent as the coefficient of determination (r2) between PEth and EtG concentrations was 0.15. However, when the EtG concentrations were evaluated for different subgroups depending on ethanol or PEth concentrations a statistically significant difference between successive higher concentrations was observed. EtG and PEth are independent measures of recent alcohol drinking reflecting different time windows. Their combined measurement in the same blood sample is possible and will provide valuable information regarding recent alcohol consumption as a complement to self-reporting.http://www.sciencedirect.com/science/article/pii/S2667145X21000201Alcohol biomarkersBloodEthanolEthyl glucuronidePhosphatidylethanolLiquid chromatography–mass spectrometry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jasna Neumann Olof Beck Michael Böttcher |
spellingShingle |
Jasna Neumann Olof Beck Michael Böttcher Phosphatidylethanol, ethyl glucuronide and ethanol in blood as complementary biomarkers for alcohol consumption Journal of Mass Spectrometry and Advances in the Clinical Lab Alcohol biomarkers Blood Ethanol Ethyl glucuronide Phosphatidylethanol Liquid chromatography–mass spectrometry |
author_facet |
Jasna Neumann Olof Beck Michael Böttcher |
author_sort |
Jasna Neumann |
title |
Phosphatidylethanol, ethyl glucuronide and ethanol in blood as complementary biomarkers for alcohol consumption |
title_short |
Phosphatidylethanol, ethyl glucuronide and ethanol in blood as complementary biomarkers for alcohol consumption |
title_full |
Phosphatidylethanol, ethyl glucuronide and ethanol in blood as complementary biomarkers for alcohol consumption |
title_fullStr |
Phosphatidylethanol, ethyl glucuronide and ethanol in blood as complementary biomarkers for alcohol consumption |
title_full_unstemmed |
Phosphatidylethanol, ethyl glucuronide and ethanol in blood as complementary biomarkers for alcohol consumption |
title_sort |
phosphatidylethanol, ethyl glucuronide and ethanol in blood as complementary biomarkers for alcohol consumption |
publisher |
Elsevier |
series |
Journal of Mass Spectrometry and Advances in the Clinical Lab |
issn |
2667-145X |
publishDate |
2021-11-01 |
description |
Alcohol biomarkers can monitor both recent and long-term drinking and provide information about drinking habits as a complement to self-reporting. Ethyl glucuronide (EtG) and phosphatidylethanol (PEth) are the most sensitive available biomarkers for this purpose. The present study aimed to collect data on both PEth and EtG in the same blood sample, in addition to ethanol, in order to evaluate the combined use of these biomarkers. Venous EDTA blood samples (n = 1149) sent to the laboratory as part of a clinical routine service for measuring PEth were investigated. PEth and EtG concentrations were analyzed using liquid chromatography–mass spectrometry methods and ethanol with an enzymatic method. Of the 1149 samples, 95 were positive for ethanol (range 0.11–3.12 g/L), 454 for EtG (1.0–9739 ng/mL), 635 for PEth (0.014–6.0 µmol/L), 534 for PEth ≥ 0.050 µmol/L, and 315 for PEth ≥ 0.30 µmol/L. EtG and PEth concentrations seemed largely independent as the coefficient of determination (r2) between PEth and EtG concentrations was 0.15. However, when the EtG concentrations were evaluated for different subgroups depending on ethanol or PEth concentrations a statistically significant difference between successive higher concentrations was observed. EtG and PEth are independent measures of recent alcohol drinking reflecting different time windows. Their combined measurement in the same blood sample is possible and will provide valuable information regarding recent alcohol consumption as a complement to self-reporting. |
topic |
Alcohol biomarkers Blood Ethanol Ethyl glucuronide Phosphatidylethanol Liquid chromatography–mass spectrometry |
url |
http://www.sciencedirect.com/science/article/pii/S2667145X21000201 |
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