A Targeted ”Next-Generation” Sequencing-Informatic Approach to Define Genetic Diversity in <i>Theileria orientalis</i> Populations within Individual Cattle: Proof-of-Principle
Oriental theileriosis is an economically important tickborne disease of bovines, caused by some members of the <i>Theileria orientalis</i> complex. Currently, 11 distinct operational taxonomic units (OTUs), or genotypes, are recognized based on their major piroplasm surface protein (<...
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doaj-31512258b3aa4ca4bb8c31198863944f2020-11-25T03:11:51ZengMDPI AGPathogens2076-08172020-06-01944844810.3390/pathogens9060448A Targeted ”Next-Generation” Sequencing-Informatic Approach to Define Genetic Diversity in <i>Theileria orientalis</i> Populations within Individual Cattle: Proof-of-PrincipleAnson V. Koehler0Abdul Jabbar1Ross S. Hall2Robin B. Gasser3Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, AustraliaDepartment of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, AustraliaDepartment of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, AustraliaDepartment of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, AustraliaOriental theileriosis is an economically important tickborne disease of bovines, caused by some members of the <i>Theileria orientalis</i> complex. Currently, 11 distinct operational taxonomic units (OTUs), or genotypes, are recognized based on their major piroplasm surface protein (<i>MPSP</i>) gene sequences. Two of these genotypes (i.e., <i>chitose</i> and <i>ikeda</i>) are recognized as pathogenic in cattle, causing significant disease in countries of the Asia-Pacific region. However, the true extent of genetic variation and associated virulence/pathogenicity within this complex is unknown. Here, we undertook a proof-of-principle study of a small panel of genomic DNAs (<i>n</i> = 13) from blood samples originating from individual cattle known to harbor <i>T. orientalis</i>, in order to assess the performance of a targeted ”next-generation” sequencing-informatic approach to identify genotypes. Five genotypes (<i>chitose</i>, <i>ikeda</i>, <i>buffeli</i>, <i>type 4,</i> and <i>type 5</i>) were defined; multiple genotypes were found within individual samples, with dominant and minor sequence types representing most genotypes. This study indicates that this sequencing-informatic workflow could be useful to assess the nature and extent of genetic variation within and among populations of <i>T. orientalis</i> on a large scale, and to potentially employ panels of distinct gene markers for expanded molecular epidemiological investigations of socioeconomically important protistan pathogens more generally.https://www.mdpi.com/2076-0817/9/6/448<i>Theileria orientalis</i>major piroplasm surface protein (<i>MPSP</i>) genemultiplicity of infection (MOI)next-generation sequencing (NGS)SeekDeep |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anson V. Koehler Abdul Jabbar Ross S. Hall Robin B. Gasser |
spellingShingle |
Anson V. Koehler Abdul Jabbar Ross S. Hall Robin B. Gasser A Targeted ”Next-Generation” Sequencing-Informatic Approach to Define Genetic Diversity in <i>Theileria orientalis</i> Populations within Individual Cattle: Proof-of-Principle Pathogens <i>Theileria orientalis</i> major piroplasm surface protein (<i>MPSP</i>) gene multiplicity of infection (MOI) next-generation sequencing (NGS) SeekDeep |
author_facet |
Anson V. Koehler Abdul Jabbar Ross S. Hall Robin B. Gasser |
author_sort |
Anson V. Koehler |
title |
A Targeted ”Next-Generation” Sequencing-Informatic Approach to Define Genetic Diversity in <i>Theileria orientalis</i> Populations within Individual Cattle: Proof-of-Principle |
title_short |
A Targeted ”Next-Generation” Sequencing-Informatic Approach to Define Genetic Diversity in <i>Theileria orientalis</i> Populations within Individual Cattle: Proof-of-Principle |
title_full |
A Targeted ”Next-Generation” Sequencing-Informatic Approach to Define Genetic Diversity in <i>Theileria orientalis</i> Populations within Individual Cattle: Proof-of-Principle |
title_fullStr |
A Targeted ”Next-Generation” Sequencing-Informatic Approach to Define Genetic Diversity in <i>Theileria orientalis</i> Populations within Individual Cattle: Proof-of-Principle |
title_full_unstemmed |
A Targeted ”Next-Generation” Sequencing-Informatic Approach to Define Genetic Diversity in <i>Theileria orientalis</i> Populations within Individual Cattle: Proof-of-Principle |
title_sort |
targeted ”next-generation” sequencing-informatic approach to define genetic diversity in <i>theileria orientalis</i> populations within individual cattle: proof-of-principle |
publisher |
MDPI AG |
series |
Pathogens |
issn |
2076-0817 |
publishDate |
2020-06-01 |
description |
Oriental theileriosis is an economically important tickborne disease of bovines, caused by some members of the <i>Theileria orientalis</i> complex. Currently, 11 distinct operational taxonomic units (OTUs), or genotypes, are recognized based on their major piroplasm surface protein (<i>MPSP</i>) gene sequences. Two of these genotypes (i.e., <i>chitose</i> and <i>ikeda</i>) are recognized as pathogenic in cattle, causing significant disease in countries of the Asia-Pacific region. However, the true extent of genetic variation and associated virulence/pathogenicity within this complex is unknown. Here, we undertook a proof-of-principle study of a small panel of genomic DNAs (<i>n</i> = 13) from blood samples originating from individual cattle known to harbor <i>T. orientalis</i>, in order to assess the performance of a targeted ”next-generation” sequencing-informatic approach to identify genotypes. Five genotypes (<i>chitose</i>, <i>ikeda</i>, <i>buffeli</i>, <i>type 4,</i> and <i>type 5</i>) were defined; multiple genotypes were found within individual samples, with dominant and minor sequence types representing most genotypes. This study indicates that this sequencing-informatic workflow could be useful to assess the nature and extent of genetic variation within and among populations of <i>T. orientalis</i> on a large scale, and to potentially employ panels of distinct gene markers for expanded molecular epidemiological investigations of socioeconomically important protistan pathogens more generally. |
topic |
<i>Theileria orientalis</i> major piroplasm surface protein (<i>MPSP</i>) gene multiplicity of infection (MOI) next-generation sequencing (NGS) SeekDeep |
url |
https://www.mdpi.com/2076-0817/9/6/448 |
work_keys_str_mv |
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