Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from Mexico
Tuberculosis (TB) is an infectocontagious respiratory disease caused by members of the Mycobacterium tuberculosis complex. A 7 base pair (bp) deletion in the locus polyketide synthase (pks)15/1 is described as polymorphic among members of the M. tuberculosis complex, enabling the identification of E...
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Instituto Oswaldo Cruz, Ministério da Saúde
2013-09-01
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doaj-317738a0b11d4f60857f82ce12d2fab72020-11-25T00:12:45ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.1678-80602013-09-01108671872310.1590/0074-0276108062013007S0074-02762013000600718Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from MexicoRoberto Zenteno-CuevasFrancisco X Silva-HernandezFabiola Mendoza-DamianMaria Dolores Ramirez-HernandezKaren Vazquez-MedinaLorena Widrobo-GarciaAremy Cuellar-SanchezRaquel Muniz-SalazarLeonor Enciso-MorenoLucia Monserrat Perez-NavarroJose Antonio Enciso-MorenoTuberculosis (TB) is an infectocontagious respiratory disease caused by members of the Mycobacterium tuberculosis complex. A 7 base pair (bp) deletion in the locus polyketide synthase (pks)15/1 is described as polymorphic among members of the M. tuberculosis complex, enabling the identification of Euro-American, Indo-Oceanic and Asian lineages. The aim of this study was to characterise this locus in TB isolates from Mexico. One hundred twenty clinical isolates were recovered from the states of Veracruz and Estado de Mexico. We determined the nucleotide sequence of a ± 400 bp fragment of the locus pks15/1, while genotypic characterisation was performed by spoligotyping. One hundred and fifty isolates contained the 7 bp deletion, while five had the wild type locus. Lineages X (22%), LAM (18%) and T (17%) were the most frequent; only three (2%) of the isolates were identified as Beijing and two (1%) EAI-Manila. The wild type pks15/1 locus was observed in all Asian lineage isolates tested. Our results confirm the utility of locus pks15/1 as a molecular marker for identifying Asian lineages of the M. tuberculosis complex. This marker could be of great value in the epidemiological surveillance of TB, especially in countries like Mexico, where the prevalence of such lineages is unknown.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000600718&lng=en&tlng=entuberculosisW-Beijingpks15/1Mexico |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Roberto Zenteno-Cuevas Francisco X Silva-Hernandez Fabiola Mendoza-Damian Maria Dolores Ramirez-Hernandez Karen Vazquez-Medina Lorena Widrobo-Garcia Aremy Cuellar-Sanchez Raquel Muniz-Salazar Leonor Enciso-Moreno Lucia Monserrat Perez-Navarro Jose Antonio Enciso-Moreno |
spellingShingle |
Roberto Zenteno-Cuevas Francisco X Silva-Hernandez Fabiola Mendoza-Damian Maria Dolores Ramirez-Hernandez Karen Vazquez-Medina Lorena Widrobo-Garcia Aremy Cuellar-Sanchez Raquel Muniz-Salazar Leonor Enciso-Moreno Lucia Monserrat Perez-Navarro Jose Antonio Enciso-Moreno Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from Mexico Memórias do Instituto Oswaldo Cruz. tuberculosis W-Beijing pks15/1 Mexico |
author_facet |
Roberto Zenteno-Cuevas Francisco X Silva-Hernandez Fabiola Mendoza-Damian Maria Dolores Ramirez-Hernandez Karen Vazquez-Medina Lorena Widrobo-Garcia Aremy Cuellar-Sanchez Raquel Muniz-Salazar Leonor Enciso-Moreno Lucia Monserrat Perez-Navarro Jose Antonio Enciso-Moreno |
author_sort |
Roberto Zenteno-Cuevas |
title |
Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from Mexico |
title_short |
Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from Mexico |
title_full |
Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from Mexico |
title_fullStr |
Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from Mexico |
title_full_unstemmed |
Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from Mexico |
title_sort |
characterisation of pks15/1 in clinical isolates of mycobacterium tuberculosis from mexico |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
series |
Memórias do Instituto Oswaldo Cruz. |
issn |
1678-8060 |
publishDate |
2013-09-01 |
description |
Tuberculosis (TB) is an infectocontagious respiratory disease caused by members of the Mycobacterium tuberculosis complex. A 7 base pair (bp) deletion in the locus polyketide synthase (pks)15/1 is described as polymorphic among members of the M. tuberculosis complex, enabling the identification of Euro-American, Indo-Oceanic and Asian lineages. The aim of this study was to characterise this locus in TB isolates from Mexico. One hundred twenty clinical isolates were recovered from the states of Veracruz and Estado de Mexico. We determined the nucleotide sequence of a ± 400 bp fragment of the locus pks15/1, while genotypic characterisation was performed by spoligotyping. One hundred and fifty isolates contained the 7 bp deletion, while five had the wild type locus. Lineages X (22%), LAM (18%) and T (17%) were the most frequent; only three (2%) of the isolates were identified as Beijing and two (1%) EAI-Manila. The wild type pks15/1 locus was observed in all Asian lineage isolates tested. Our results confirm the utility of locus pks15/1 as a molecular marker for identifying Asian lineages of the M. tuberculosis complex. This marker could be of great value in the epidemiological surveillance of TB, especially in countries like Mexico, where the prevalence of such lineages is unknown. |
topic |
tuberculosis W-Beijing pks15/1 Mexico |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000600718&lng=en&tlng=en |
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