Metabolomics by UHPLC-Q-TOF Reveals Host Tree-Dependent Phytochemical Variation in <i>Viscum album</i> L.
<i>Viscum album</i> L., commonly known as European mistletoe, is a hemi-parasitic plant of the Santalaceae family. The in vitro and in vivo effects of <i>V. album</i> differ, according to its host tree. However, little is known about the host-dependent phytochemical diversity...
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doaj-318fbb0421354f3dbe280e8c2d97d9da2021-08-26T14:14:38ZengMDPI AGPlants2223-77472021-08-01101726172610.3390/plants10081726Metabolomics by UHPLC-Q-TOF Reveals Host Tree-Dependent Phytochemical Variation in <i>Viscum album</i> L.Tim Jäger0Carla Holandino1Michelle Nonato de Oliveira Melo2Evelyn Maribel Condori Peñaloza3Adriana Passos Oliveira4Rafael Garrett5Gaétan Glauser6Mirio Grazi7Hartmut Ramm8Konrad Urech9Stephan Baumgartner10Society for Cancer Research, Hiscia Institute, Kirschweg 9, 4144 Arlesheim, SwitzerlandSociety for Cancer Research, Hiscia Institute, Kirschweg 9, 4144 Arlesheim, SwitzerlandLaboratório Multidisciplinar de Ciências Farmacêuticas, Pharmacy College, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, BrazilLaboratório Multidisciplinar de Ciências Farmacêuticas, Pharmacy College, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, BrazilLaboratório Multidisciplinar de Ciências Farmacêuticas, Pharmacy College, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, BrazilMetabolomics Laboratory, Chemistry Institute, Federal University of Rio de Janeiro, Rio de Janeiro 21941-598, BrazilNeuchatel Platform of Analytical Chemistry, University of Neuchâtel, Avenue de Bellevaux 51, 2000 Neuchâtel, SwitzerlandSociety for Cancer Research, Hiscia Institute, Kirschweg 9, 4144 Arlesheim, SwitzerlandSociety for Cancer Research, Hiscia Institute, Kirschweg 9, 4144 Arlesheim, SwitzerlandSociety for Cancer Research, Hiscia Institute, Kirschweg 9, 4144 Arlesheim, SwitzerlandSociety for Cancer Research, Hiscia Institute, Kirschweg 9, 4144 Arlesheim, Switzerland<i>Viscum album</i> L., commonly known as European mistletoe, is a hemi-parasitic plant of the Santalaceae family. The in vitro and in vivo effects of <i>V. album</i> differ, according to its host tree. However, little is known about the host-dependent phytochemical diversity in <i>V. album</i>. In this study, the metabolic profiles of <i>V. album</i> ssp. <i>album</i> from <i>Malus domestica</i> Bork., <i>Quercus robur</i> L., and <i>Ulmus carpinifolia</i> Gled were compared. Leaves, stems, and berries were collected in Switzerland, by the same procedure, in September 2016 and 2017. The methanolic extracts were analyzed by ultra-performance liquid chromatography, coupled to electrospray quadrupole time-of-flight mass spectrometry in positive ionization mode. The data were submitted to partial-least square discriminant analysis (PLS-DA) and the results showed that the <i>V. album</i> ssp. <i>album</i> samples were clustered into three groups, according to the three distinct host trees. Seven compounds, with high VIP scores (variable importance in projection), were responsible for this differentiation. The following four compounds were detected in both the harvest years: arginine, pipecolic acid or lysine, dimethoxycoumarin, and sinapyl alcohol, suggesting their use as host specific <i>V. album</i> biomarkers. The present work highlights the importance of standardized harvest and analytical procedures for the reproducibility of the chemical results of herbal materials.https://www.mdpi.com/2223-7747/10/8/1726<i>Viscum album</i>mistletoehost treemetabolite fingerprintingmultivariate analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tim Jäger Carla Holandino Michelle Nonato de Oliveira Melo Evelyn Maribel Condori Peñaloza Adriana Passos Oliveira Rafael Garrett Gaétan Glauser Mirio Grazi Hartmut Ramm Konrad Urech Stephan Baumgartner |
spellingShingle |
Tim Jäger Carla Holandino Michelle Nonato de Oliveira Melo Evelyn Maribel Condori Peñaloza Adriana Passos Oliveira Rafael Garrett Gaétan Glauser Mirio Grazi Hartmut Ramm Konrad Urech Stephan Baumgartner Metabolomics by UHPLC-Q-TOF Reveals Host Tree-Dependent Phytochemical Variation in <i>Viscum album</i> L. Plants <i>Viscum album</i> mistletoe host tree metabolite fingerprinting multivariate analysis |
author_facet |
Tim Jäger Carla Holandino Michelle Nonato de Oliveira Melo Evelyn Maribel Condori Peñaloza Adriana Passos Oliveira Rafael Garrett Gaétan Glauser Mirio Grazi Hartmut Ramm Konrad Urech Stephan Baumgartner |
author_sort |
Tim Jäger |
title |
Metabolomics by UHPLC-Q-TOF Reveals Host Tree-Dependent Phytochemical Variation in <i>Viscum album</i> L. |
title_short |
Metabolomics by UHPLC-Q-TOF Reveals Host Tree-Dependent Phytochemical Variation in <i>Viscum album</i> L. |
title_full |
Metabolomics by UHPLC-Q-TOF Reveals Host Tree-Dependent Phytochemical Variation in <i>Viscum album</i> L. |
title_fullStr |
Metabolomics by UHPLC-Q-TOF Reveals Host Tree-Dependent Phytochemical Variation in <i>Viscum album</i> L. |
title_full_unstemmed |
Metabolomics by UHPLC-Q-TOF Reveals Host Tree-Dependent Phytochemical Variation in <i>Viscum album</i> L. |
title_sort |
metabolomics by uhplc-q-tof reveals host tree-dependent phytochemical variation in <i>viscum album</i> l. |
publisher |
MDPI AG |
series |
Plants |
issn |
2223-7747 |
publishDate |
2021-08-01 |
description |
<i>Viscum album</i> L., commonly known as European mistletoe, is a hemi-parasitic plant of the Santalaceae family. The in vitro and in vivo effects of <i>V. album</i> differ, according to its host tree. However, little is known about the host-dependent phytochemical diversity in <i>V. album</i>. In this study, the metabolic profiles of <i>V. album</i> ssp. <i>album</i> from <i>Malus domestica</i> Bork., <i>Quercus robur</i> L., and <i>Ulmus carpinifolia</i> Gled were compared. Leaves, stems, and berries were collected in Switzerland, by the same procedure, in September 2016 and 2017. The methanolic extracts were analyzed by ultra-performance liquid chromatography, coupled to electrospray quadrupole time-of-flight mass spectrometry in positive ionization mode. The data were submitted to partial-least square discriminant analysis (PLS-DA) and the results showed that the <i>V. album</i> ssp. <i>album</i> samples were clustered into three groups, according to the three distinct host trees. Seven compounds, with high VIP scores (variable importance in projection), were responsible for this differentiation. The following four compounds were detected in both the harvest years: arginine, pipecolic acid or lysine, dimethoxycoumarin, and sinapyl alcohol, suggesting their use as host specific <i>V. album</i> biomarkers. The present work highlights the importance of standardized harvest and analytical procedures for the reproducibility of the chemical results of herbal materials. |
topic |
<i>Viscum album</i> mistletoe host tree metabolite fingerprinting multivariate analysis |
url |
https://www.mdpi.com/2223-7747/10/8/1726 |
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