N-acetyl-L-cysteine Prevents Lactate-Mediated PGC1-alpha Expression in C2C12 Myotubes
Background: Exercise induces many physiological adaptations. Recently, it has been proposed that some of these adaptations are induced by exercise-mediated lactate production. In this study, we aimed to investigate in vitro the effect of lactate in cultured myotubes and whether antioxidants could in...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2019-06-01
|
Series: | Biology |
Subjects: | |
Online Access: | https://www.mdpi.com/2079-7737/8/2/44 |
id |
doaj-31a4a799067742fbb849226078ff4c97 |
---|---|
record_format |
Article |
spelling |
doaj-31a4a799067742fbb849226078ff4c972020-11-25T00:31:13ZengMDPI AGBiology2079-77372019-06-01824410.3390/biology8020044biology8020044N-acetyl-L-cysteine Prevents Lactate-Mediated PGC1-alpha Expression in C2C12 MyotubesMinas Nalbandian0Zsolt Radak1Masaki Takeda2Graduate School of Medicine, Kyoto University, Kyoto 606-8315, JapanResearch Institute of Sport Science, University of Physical Education, 1525 Budapest, HungaryGraduate School of Sports and Health Science, Doshisha University, Kyotanabe 610-0394, JapanBackground: Exercise induces many physiological adaptations. Recently, it has been proposed that some of these adaptations are induced by exercise-mediated lactate production. In this study, we aimed to investigate in vitro the effect of lactate in cultured myotubes and whether antioxidants could inhibit the effect. Methods: Differentiated myotubes were cultured at different concentrations of L-lactate (0, 10, 30, 50 mM) in the absence or presence of an antioxidant, N-acetyl-L-cysteine (Nac). The temporal effect of lactate exposure in myotubes was also explored. Results: Two hours of exposure to 50 mM L-lactate and six hours of exposure to 30 or 50 mM L-lactate caused a significant increase in PGC1-alpha (peroxisome proliferator-activated receptor γ coactivator-1α) expression in the myotubes. This up-regulation was suppressed by 2 mM Nac. Intermittent and continuous lactate exposure caused similar PGC1-alpha up-regulation. These results suggest that the increase in PGC1-alpha expression is mediated by reactive oxygen species (ROS) production from lactate metabolism and that both continuous and intermittent exposure to L-lactate can cause the up-regulation.https://www.mdpi.com/2079-7737/8/2/44lactate signalingROSPGC1-alphamyotubes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Minas Nalbandian Zsolt Radak Masaki Takeda |
spellingShingle |
Minas Nalbandian Zsolt Radak Masaki Takeda N-acetyl-L-cysteine Prevents Lactate-Mediated PGC1-alpha Expression in C2C12 Myotubes Biology lactate signaling ROS PGC1-alpha myotubes |
author_facet |
Minas Nalbandian Zsolt Radak Masaki Takeda |
author_sort |
Minas Nalbandian |
title |
N-acetyl-L-cysteine Prevents Lactate-Mediated PGC1-alpha Expression in C2C12 Myotubes |
title_short |
N-acetyl-L-cysteine Prevents Lactate-Mediated PGC1-alpha Expression in C2C12 Myotubes |
title_full |
N-acetyl-L-cysteine Prevents Lactate-Mediated PGC1-alpha Expression in C2C12 Myotubes |
title_fullStr |
N-acetyl-L-cysteine Prevents Lactate-Mediated PGC1-alpha Expression in C2C12 Myotubes |
title_full_unstemmed |
N-acetyl-L-cysteine Prevents Lactate-Mediated PGC1-alpha Expression in C2C12 Myotubes |
title_sort |
n-acetyl-l-cysteine prevents lactate-mediated pgc1-alpha expression in c2c12 myotubes |
publisher |
MDPI AG |
series |
Biology |
issn |
2079-7737 |
publishDate |
2019-06-01 |
description |
Background: Exercise induces many physiological adaptations. Recently, it has been proposed that some of these adaptations are induced by exercise-mediated lactate production. In this study, we aimed to investigate in vitro the effect of lactate in cultured myotubes and whether antioxidants could inhibit the effect. Methods: Differentiated myotubes were cultured at different concentrations of L-lactate (0, 10, 30, 50 mM) in the absence or presence of an antioxidant, N-acetyl-L-cysteine (Nac). The temporal effect of lactate exposure in myotubes was also explored. Results: Two hours of exposure to 50 mM L-lactate and six hours of exposure to 30 or 50 mM L-lactate caused a significant increase in PGC1-alpha (peroxisome proliferator-activated receptor γ coactivator-1α) expression in the myotubes. This up-regulation was suppressed by 2 mM Nac. Intermittent and continuous lactate exposure caused similar PGC1-alpha up-regulation. These results suggest that the increase in PGC1-alpha expression is mediated by reactive oxygen species (ROS) production from lactate metabolism and that both continuous and intermittent exposure to L-lactate can cause the up-regulation. |
topic |
lactate signaling ROS PGC1-alpha myotubes |
url |
https://www.mdpi.com/2079-7737/8/2/44 |
work_keys_str_mv |
AT minasnalbandian nacetyllcysteinepreventslactatemediatedpgc1alphaexpressioninc2c12myotubes AT zsoltradak nacetyllcysteinepreventslactatemediatedpgc1alphaexpressioninc2c12myotubes AT masakitakeda nacetyllcysteinepreventslactatemediatedpgc1alphaexpressioninc2c12myotubes |
_version_ |
1725323063576231936 |