Metabolism of apical versus basolateral sn-2-monoacylglycerol and fatty acids in rodent small intestine*
The metabolic fates of radiolabeled sn-2-monoacylglycerol (MG) and oleate (FA) in rat and mouse intestine, added in vivo to the apical (AP) surface in bile salt micelles, or to the basolateral (BL) surface via albumin-bound solution, were examined. Mucosal lipid products were quantified, and the res...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2008-08-01
|
Series: | Journal of Lipid Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520346952 |
id |
doaj-31aad2c1a70a4c4e928ea7a276e0471c |
---|---|
record_format |
Article |
spelling |
doaj-31aad2c1a70a4c4e928ea7a276e0471c2021-04-28T05:58:35ZengElsevierJournal of Lipid Research0022-22752008-08-0149817621769Metabolism of apical versus basolateral sn-2-monoacylglycerol and fatty acids in rodent small intestine*Judith Storch0Yin Xiu Zhou1William S. Lagakos2Department of Nutritional Sciences and Rutgers Center for Lipid Research, School of Environmental and Biological Sciences, Rutgers University, New Brunswick, NJ 08901Department of Nutritional Sciences and Rutgers Center for Lipid Research, School of Environmental and Biological Sciences, Rutgers University, New Brunswick, NJ 08901Department of Nutritional Sciences and Rutgers Center for Lipid Research, School of Environmental and Biological Sciences, Rutgers University, New Brunswick, NJ 08901The metabolic fates of radiolabeled sn-2-monoacylglycerol (MG) and oleate (FA) in rat and mouse intestine, added in vivo to the apical (AP) surface in bile salt micelles, or to the basolateral (BL) surface via albumin-bound solution, were examined. Mucosal lipid products were quantified, and the results demonstrate a dramatic difference in the esterification patterns for both MG and FA, depending upon their site of entry into the enterocyte. For both lipids, the ratio of triacylglycerol to phospholipid (TG:PL) formed was approximately 10-fold higher for delivery at the AP relative to the BL surface. Further, a 3-fold higher level of FA oxidation was found for BL compared with AP substrate delivery. Incorporation of FA into individual PL species was also significantly different, with >2-fold greater incorporation into phosphatidylethanolamine (PE) and a 3-fold decrease in the phosphatidylcholine:PE ratio for AP- compared with BL-added lipid. Overnight fasting increased the TG:PL incorporation ratio for both AP and BL lipid addition, suggesting that metabolic compartmentation is a physiologically regulated phenomenon. These results support the existence of separate pools of TG and glycerolipid intermediates in the intestinal epithelial cell, and underscore the importance of substrate trafficking in the regulation of enterocyte lipid metabolism.http://www.sciencedirect.com/science/article/pii/S0022227520346952fatty acidenterocyteepithelial celllipid metabolismoxidationtriacylglycerol |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Judith Storch Yin Xiu Zhou William S. Lagakos |
spellingShingle |
Judith Storch Yin Xiu Zhou William S. Lagakos Metabolism of apical versus basolateral sn-2-monoacylglycerol and fatty acids in rodent small intestine* Journal of Lipid Research fatty acid enterocyte epithelial cell lipid metabolism oxidation triacylglycerol |
author_facet |
Judith Storch Yin Xiu Zhou William S. Lagakos |
author_sort |
Judith Storch |
title |
Metabolism of apical versus basolateral sn-2-monoacylglycerol and fatty acids in rodent small intestine* |
title_short |
Metabolism of apical versus basolateral sn-2-monoacylglycerol and fatty acids in rodent small intestine* |
title_full |
Metabolism of apical versus basolateral sn-2-monoacylglycerol and fatty acids in rodent small intestine* |
title_fullStr |
Metabolism of apical versus basolateral sn-2-monoacylglycerol and fatty acids in rodent small intestine* |
title_full_unstemmed |
Metabolism of apical versus basolateral sn-2-monoacylglycerol and fatty acids in rodent small intestine* |
title_sort |
metabolism of apical versus basolateral sn-2-monoacylglycerol and fatty acids in rodent small intestine* |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2008-08-01 |
description |
The metabolic fates of radiolabeled sn-2-monoacylglycerol (MG) and oleate (FA) in rat and mouse intestine, added in vivo to the apical (AP) surface in bile salt micelles, or to the basolateral (BL) surface via albumin-bound solution, were examined. Mucosal lipid products were quantified, and the results demonstrate a dramatic difference in the esterification patterns for both MG and FA, depending upon their site of entry into the enterocyte. For both lipids, the ratio of triacylglycerol to phospholipid (TG:PL) formed was approximately 10-fold higher for delivery at the AP relative to the BL surface. Further, a 3-fold higher level of FA oxidation was found for BL compared with AP substrate delivery. Incorporation of FA into individual PL species was also significantly different, with >2-fold greater incorporation into phosphatidylethanolamine (PE) and a 3-fold decrease in the phosphatidylcholine:PE ratio for AP- compared with BL-added lipid. Overnight fasting increased the TG:PL incorporation ratio for both AP and BL lipid addition, suggesting that metabolic compartmentation is a physiologically regulated phenomenon. These results support the existence of separate pools of TG and glycerolipid intermediates in the intestinal epithelial cell, and underscore the importance of substrate trafficking in the regulation of enterocyte lipid metabolism. |
topic |
fatty acid enterocyte epithelial cell lipid metabolism oxidation triacylglycerol |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520346952 |
work_keys_str_mv |
AT judithstorch metabolismofapicalversusbasolateralsn2monoacylglycerolandfattyacidsinrodentsmallintestine AT yinxiuzhou metabolismofapicalversusbasolateralsn2monoacylglycerolandfattyacidsinrodentsmallintestine AT williamslagakos metabolismofapicalversusbasolateralsn2monoacylglycerolandfattyacidsinrodentsmallintestine |
_version_ |
1721504679009976320 |