A Discovery of Relevant Hepatoprotective Effects and Underlying Mechanisms of Dietary <i>Clostridium butyricum</i> Against Corticosterone-Induced Liver Injury in Pekin Ducks
<i>Clostridium butyricum</i> (<i>C. butyricum</i>) can attenuate oxidative stress, inflammation, and hepatic fatty deposition in poultry, however, the underlying mechanisms for this in Pekin ducks remain unclear. This study evaluated these hepatoprotective effects and the und...
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doaj-31adc6dd7613471cab1d07f2425bdf902020-11-24T21:26:40ZengMDPI AGMicroorganisms2076-26072019-09-017935810.3390/microorganisms7090358microorganisms7090358A Discovery of Relevant Hepatoprotective Effects and Underlying Mechanisms of Dietary <i>Clostridium butyricum</i> Against Corticosterone-Induced Liver Injury in Pekin DucksYanhan Liu0Cun Liu1Liqing Huang2Zhaofei Xia3College of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaCollege of Veterinary Medicine, China Agricultural University, Beijing 100193, ChinaCollege of Veterinary Medicine, China Agricultural University, Beijing 100193, China<i>Clostridium butyricum</i> (<i>C. butyricum</i>) can attenuate oxidative stress, inflammation, and hepatic fatty deposition in poultry, however, the underlying mechanisms for this in Pekin ducks remain unclear. This study evaluated these hepatoprotective effects and the underlying mechanisms in a corticosterone (CORT)-induced liver injury model in Pekin ducks fed a <i>C. butyricum</i> intervention diet. A total of 500 Pekin ducks were randomly divided into five groups: one group (CON group) was only provided with a basal diet, three groups were provided a basal diet with 200 mg/kg (LCB group), 400 mg/kg (MCB group), or 600 mg/kg (HCB group) <i>C. butyricum</i>, respectively, and one group was provided a basal diet with 150 mg/kg aureomycin (ANT group) for 42 d. At 37 days-old, all ducks received daily intraperitoneal injections of CORT for five days to establish a liver injury model. <i>C. butyricum</i> intervention alleviated liver injury by decreasing the liver organ indices, hepatic steatosis and hepatocyte necrosis, and improving liver function, antioxidant capacity, and inflammatory factors. Hepatic RNA-seq revealed 365 differentially expressed genes (DEGs) between the MCB and CON groups, with 229 up- and 136 down-regulated DEGs in the MCB group. Between the MCB and ANT groups, 407 DEGs were identified, including 299 up- and 108 down-regulated genes in MCB group. Some DEGs in the MCB group related to oxidative stress and inflammatory responses such as Sod3, <i>Tlr2a/b</i>, and <i>Il10,</i> which were up-regulated, while <i>Apoa1</i>, <i>Cyp7a1</i>, <i>Acsl1/5</i>, <i>Fasn</i>, <i>Ppar-γ</i>, and <i>Scd</i>, which are involved in lipid metabolism, were down-regulated, indicating that these genes were responsive to dietary <i>C. butyricum</i> for the alleviation of corticosterone-induced hepatic injury. Toll-like receptor signaling, PI3K-Akt signaling pathway, cytokine-cytokine receptor interaction, peroxisome proliferator-activated receptor (PPAR) signaling pathway, adipocytokine and glycerophospholipid metabolism signaling pathway were significantly enriched in the MCB group. These findings indicate that <i>C. butyricum</i> intervention can protect Pekin ducks from corticosterone-induced liver injury by the modulation of immunoregulatory- and lipid metabolism-related genes and pathways.https://www.mdpi.com/2076-2607/7/9/358corticosterone<i>Clostridium butyricum</i>liverRNA-seqPekin ducks |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yanhan Liu Cun Liu Liqing Huang Zhaofei Xia |
spellingShingle |
Yanhan Liu Cun Liu Liqing Huang Zhaofei Xia A Discovery of Relevant Hepatoprotective Effects and Underlying Mechanisms of Dietary <i>Clostridium butyricum</i> Against Corticosterone-Induced Liver Injury in Pekin Ducks Microorganisms corticosterone <i>Clostridium butyricum</i> liver RNA-seq Pekin ducks |
author_facet |
Yanhan Liu Cun Liu Liqing Huang Zhaofei Xia |
author_sort |
Yanhan Liu |
title |
A Discovery of Relevant Hepatoprotective Effects and Underlying Mechanisms of Dietary <i>Clostridium butyricum</i> Against Corticosterone-Induced Liver Injury in Pekin Ducks |
title_short |
A Discovery of Relevant Hepatoprotective Effects and Underlying Mechanisms of Dietary <i>Clostridium butyricum</i> Against Corticosterone-Induced Liver Injury in Pekin Ducks |
title_full |
A Discovery of Relevant Hepatoprotective Effects and Underlying Mechanisms of Dietary <i>Clostridium butyricum</i> Against Corticosterone-Induced Liver Injury in Pekin Ducks |
title_fullStr |
A Discovery of Relevant Hepatoprotective Effects and Underlying Mechanisms of Dietary <i>Clostridium butyricum</i> Against Corticosterone-Induced Liver Injury in Pekin Ducks |
title_full_unstemmed |
A Discovery of Relevant Hepatoprotective Effects and Underlying Mechanisms of Dietary <i>Clostridium butyricum</i> Against Corticosterone-Induced Liver Injury in Pekin Ducks |
title_sort |
discovery of relevant hepatoprotective effects and underlying mechanisms of dietary <i>clostridium butyricum</i> against corticosterone-induced liver injury in pekin ducks |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2019-09-01 |
description |
<i>Clostridium butyricum</i> (<i>C. butyricum</i>) can attenuate oxidative stress, inflammation, and hepatic fatty deposition in poultry, however, the underlying mechanisms for this in Pekin ducks remain unclear. This study evaluated these hepatoprotective effects and the underlying mechanisms in a corticosterone (CORT)-induced liver injury model in Pekin ducks fed a <i>C. butyricum</i> intervention diet. A total of 500 Pekin ducks were randomly divided into five groups: one group (CON group) was only provided with a basal diet, three groups were provided a basal diet with 200 mg/kg (LCB group), 400 mg/kg (MCB group), or 600 mg/kg (HCB group) <i>C. butyricum</i>, respectively, and one group was provided a basal diet with 150 mg/kg aureomycin (ANT group) for 42 d. At 37 days-old, all ducks received daily intraperitoneal injections of CORT for five days to establish a liver injury model. <i>C. butyricum</i> intervention alleviated liver injury by decreasing the liver organ indices, hepatic steatosis and hepatocyte necrosis, and improving liver function, antioxidant capacity, and inflammatory factors. Hepatic RNA-seq revealed 365 differentially expressed genes (DEGs) between the MCB and CON groups, with 229 up- and 136 down-regulated DEGs in the MCB group. Between the MCB and ANT groups, 407 DEGs were identified, including 299 up- and 108 down-regulated genes in MCB group. Some DEGs in the MCB group related to oxidative stress and inflammatory responses such as Sod3, <i>Tlr2a/b</i>, and <i>Il10,</i> which were up-regulated, while <i>Apoa1</i>, <i>Cyp7a1</i>, <i>Acsl1/5</i>, <i>Fasn</i>, <i>Ppar-γ</i>, and <i>Scd</i>, which are involved in lipid metabolism, were down-regulated, indicating that these genes were responsive to dietary <i>C. butyricum</i> for the alleviation of corticosterone-induced hepatic injury. Toll-like receptor signaling, PI3K-Akt signaling pathway, cytokine-cytokine receptor interaction, peroxisome proliferator-activated receptor (PPAR) signaling pathway, adipocytokine and glycerophospholipid metabolism signaling pathway were significantly enriched in the MCB group. These findings indicate that <i>C. butyricum</i> intervention can protect Pekin ducks from corticosterone-induced liver injury by the modulation of immunoregulatory- and lipid metabolism-related genes and pathways. |
topic |
corticosterone <i>Clostridium butyricum</i> liver RNA-seq Pekin ducks |
url |
https://www.mdpi.com/2076-2607/7/9/358 |
work_keys_str_mv |
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