Incidence and Susceptibility of Uropathogens Isolated among the Patients at Tertiary Care Hospital in Eastern Nepal
Background Urinary Tract Infection (UTI) is one of the most common infectious diseases which affect almost all ages groups of population. Production of â-lactamases is responsible for antibacterial resistance which is frequently observed in Enterobacteriaceae isolates, particularly by E. coli and Kl...
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doaj-31b9176172fe49e8b6e14dcada6c3a6b2020-11-25T01:21:23ZengNobel Medical College Teaching HospitalJournal of Nobel Medical College2091-23312091-234X2016-12-0152515510.3126/jonmc.v5i2.1631816318Incidence and Susceptibility of Uropathogens Isolated among the Patients at Tertiary Care Hospital in Eastern NepalBigu Kumar Chaudhari0Ganesh Kumar Singh1Kamal Prasad Parajuli2Kewal Shrestha3Department of Microbiology, Nobel Medical College Teaching Hospital, BiratnagarDepartment of Microbiology, Nobel Medical College Teaching Hospital, BiratnagarDepartment of Microbiology, Nobel Medical College Teaching Hospital, BiratnagarDepartment of Microbiology, Nobel Medical College Teaching Hospital, BiratnagarBackground Urinary Tract Infection (UTI) is one of the most common infectious diseases which affect almost all ages groups of population. Production of â-lactamases is responsible for antibacterial resistance which is frequently observed in Enterobacteriaceae isolates, particularly by E. coli and Klebsiella pneumoniae. This investigation has been carried out to determine the current status of prevalence and susceptibility of uropathogens isolated among the patients at tertiary care hospital in eastern Nepal. Material and Methods This study was done at the department of Microbiology, Nobel Medical College Teaching Hospital, Biratnagar, Nepal during May 1st 2015 to October 31st 2015. Midstream cleancatch urine was sampled from 1730 suspected urinary tract infection patients of different age and sex groups. Uropathogens were recognized in term of standard and specific microbiological techniques and antimicrobial susceptibility pattern was determined by Kirby Bauer Disc diffusion method following Clinical and Laboratory Standards Institute (CLSI) guidelines. Results Out of 1730 suspected specimens Culture resulted a total of 761 (43.98 %) positive and 969 (56.02%) negative among that significant growths of uropathogens including 700 (91.98 %) unimicrobial and 60 (7.88 %) polymicrobial growths. In term of Gender distribution 443 (25.60 %) were male and 1287 (74.40 %) were female hence the ratio is 0.34:1, respectively. E. coli was the leading isolate (66 %), followed by Klebsiella spp. (12 %), Enterococcus spp. (8 %), Pseudomonas spp. (6 %), Acinetobacter anitratus (5 %), Proteus spp. (3 %). Conclusion The high frequency of multidrug resistance in bacterial uropathogens was seen. Principally, resistance patterns were seen higher for amoxycillin, co-trimoxazole, flouroquinolones and third-generation cephalosporins, Existing uropathogens highlights the highest rate of vulnerability to nitrofurantoin, amikacin and gentamicin which provide much better antibiotic coverage and can be adapted for practical treatment of urinary tract infections. Journal of Nobel Medical College Vol.5(2) 2016; 51-55https://www.nepjol.info/index.php/JoNMC/article/view/16318antimicrobial susceptibilitymid-stream urine (msu)uropathogensurinary tract infection (uti) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bigu Kumar Chaudhari Ganesh Kumar Singh Kamal Prasad Parajuli Kewal Shrestha |
spellingShingle |
Bigu Kumar Chaudhari Ganesh Kumar Singh Kamal Prasad Parajuli Kewal Shrestha Incidence and Susceptibility of Uropathogens Isolated among the Patients at Tertiary Care Hospital in Eastern Nepal Journal of Nobel Medical College antimicrobial susceptibility mid-stream urine (msu) uropathogens urinary tract infection (uti) |
author_facet |
Bigu Kumar Chaudhari Ganesh Kumar Singh Kamal Prasad Parajuli Kewal Shrestha |
author_sort |
Bigu Kumar Chaudhari |
title |
Incidence and Susceptibility of Uropathogens Isolated among the Patients at Tertiary Care Hospital in Eastern Nepal |
title_short |
Incidence and Susceptibility of Uropathogens Isolated among the Patients at Tertiary Care Hospital in Eastern Nepal |
title_full |
Incidence and Susceptibility of Uropathogens Isolated among the Patients at Tertiary Care Hospital in Eastern Nepal |
title_fullStr |
Incidence and Susceptibility of Uropathogens Isolated among the Patients at Tertiary Care Hospital in Eastern Nepal |
title_full_unstemmed |
Incidence and Susceptibility of Uropathogens Isolated among the Patients at Tertiary Care Hospital in Eastern Nepal |
title_sort |
incidence and susceptibility of uropathogens isolated among the patients at tertiary care hospital in eastern nepal |
publisher |
Nobel Medical College Teaching Hospital |
series |
Journal of Nobel Medical College |
issn |
2091-2331 2091-234X |
publishDate |
2016-12-01 |
description |
Background Urinary Tract Infection (UTI) is one of the most common infectious diseases which affect almost all ages groups of population. Production of â-lactamases is responsible for antibacterial resistance which is frequently observed in Enterobacteriaceae isolates, particularly by E. coli and Klebsiella pneumoniae. This investigation has been carried out to determine the current status of prevalence and susceptibility of uropathogens isolated among the patients at tertiary care hospital in eastern Nepal.
Material and Methods This study was done at the department of Microbiology, Nobel Medical College Teaching Hospital, Biratnagar, Nepal during May 1st 2015 to October 31st 2015. Midstream cleancatch urine was sampled from 1730 suspected urinary tract infection patients of different age and sex groups. Uropathogens were recognized in term of standard and specific microbiological techniques and antimicrobial susceptibility pattern was determined by Kirby Bauer Disc diffusion method following Clinical and Laboratory Standards Institute (CLSI) guidelines.
Results Out of 1730 suspected specimens Culture resulted a total of 761 (43.98 %) positive and 969 (56.02%) negative among that significant growths of uropathogens including 700 (91.98 %) unimicrobial and 60 (7.88 %) polymicrobial growths. In term of Gender distribution 443 (25.60 %) were male and 1287 (74.40 %) were female hence the ratio is 0.34:1, respectively. E. coli was the leading isolate (66 %), followed by Klebsiella spp. (12 %), Enterococcus spp. (8 %), Pseudomonas spp. (6 %), Acinetobacter anitratus (5 %), Proteus spp. (3 %).
Conclusion The high frequency of multidrug resistance in bacterial uropathogens was seen. Principally, resistance patterns were seen higher for amoxycillin, co-trimoxazole, flouroquinolones and third-generation cephalosporins, Existing uropathogens highlights the highest rate of vulnerability to nitrofurantoin, amikacin and gentamicin which provide much better antibiotic coverage and can be adapted for practical treatment of urinary tract infections.
Journal of Nobel Medical College Vol.5(2) 2016; 51-55 |
topic |
antimicrobial susceptibility mid-stream urine (msu) uropathogens urinary tract infection (uti) |
url |
https://www.nepjol.info/index.php/JoNMC/article/view/16318 |
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