Identification of Autoreactive B Cell Subpopulations in Peripheral Blood of Autoimmune Patients With Pemphigus Vulgaris
Pemphigus vulgaris (PV) is a rare blistering disease caused by IgG autoantibodies against the epidermal adhesion molecules desmoglein (Dsg)3 and Dsg1 providing a well-characterized paradigm of an antibody-mediated organ-specific autoimmune disease. In PV patients who have achieved clinical remission...
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doaj-31cce6510a874ac582f25c7158fd8e402020-11-25T01:34:40ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-06-011010.3389/fimmu.2019.01375459258Identification of Autoreactive B Cell Subpopulations in Peripheral Blood of Autoimmune Patients With Pemphigus VulgarisRobert Pollmann0Elias Walter1Thomas Schmidt2Jens Waschke3Michael Hertl4Christian Möbs5Rüdiger Eming6Department of Dermatology and Allergology, Philipps-Universität Marburg, Marburg, GermanyInstitute of Anatomy and Cell Biology, Ludwig-Maximilians-Universität München, Munich, GermanyDepartment of Dermatology and Allergology, Philipps-Universität Marburg, Marburg, GermanyInstitute of Anatomy and Cell Biology, Ludwig-Maximilians-Universität München, Munich, GermanyDepartment of Dermatology and Allergology, Philipps-Universität Marburg, Marburg, GermanyDepartment of Dermatology and Allergology, Philipps-Universität Marburg, Marburg, GermanyDepartment of Dermatology and Allergology, Philipps-Universität Marburg, Marburg, GermanyPemphigus vulgaris (PV) is a rare blistering disease caused by IgG autoantibodies against the epidermal adhesion molecules desmoglein (Dsg)3 and Dsg1 providing a well-characterized paradigm of an antibody-mediated organ-specific autoimmune disease. In PV patients who have achieved clinical remission after B cell-depleting therapy, relapses often coincide with a reoccurrence of B cells and Dsg-specific autoantibodies. Here, we analyzed Dsg3-specific B cell subpopulations (i.e., total CD19+ B cells, CD19+CD27−B cells, CD19+CD27+ memory B cells, and CD19+CD27hiCD38hi plasmablasts) in peripheral blood of both PV patients (n = 14) at different stages of disease and healthy individuals (n = 14) by flow cytometry using fluorescently labeled recombinant human Dsg3 protein. Applying this approach, Dsg3-specific B cells could be detected at low frequencies (0.11–0.53% of CD19+ B cells) and numbers of Dsg3-specific memory B cells were significantly increased in PV patients in clinical remission receiving minimal immunosuppressive therapy. Finally, we confirmed in vitro that Dsg3-reactive memory B cells were able to produce anti-Dsg3 IgG autoantibodies upon ex vivo activation. Thus, monitoring of Dsg3-specific B cells in PV is of particular interest to further characterize the immunopathogenesis of PV.https://www.frontiersin.org/article/10.3389/fimmu.2019.01375/fullautoimmunitypemphigus vulgarisdesmoglein 3B cellsflow cytometry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Robert Pollmann Elias Walter Thomas Schmidt Jens Waschke Michael Hertl Christian Möbs Rüdiger Eming |
spellingShingle |
Robert Pollmann Elias Walter Thomas Schmidt Jens Waschke Michael Hertl Christian Möbs Rüdiger Eming Identification of Autoreactive B Cell Subpopulations in Peripheral Blood of Autoimmune Patients With Pemphigus Vulgaris Frontiers in Immunology autoimmunity pemphigus vulgaris desmoglein 3 B cells flow cytometry |
author_facet |
Robert Pollmann Elias Walter Thomas Schmidt Jens Waschke Michael Hertl Christian Möbs Rüdiger Eming |
author_sort |
Robert Pollmann |
title |
Identification of Autoreactive B Cell Subpopulations in Peripheral Blood of Autoimmune Patients With Pemphigus Vulgaris |
title_short |
Identification of Autoreactive B Cell Subpopulations in Peripheral Blood of Autoimmune Patients With Pemphigus Vulgaris |
title_full |
Identification of Autoreactive B Cell Subpopulations in Peripheral Blood of Autoimmune Patients With Pemphigus Vulgaris |
title_fullStr |
Identification of Autoreactive B Cell Subpopulations in Peripheral Blood of Autoimmune Patients With Pemphigus Vulgaris |
title_full_unstemmed |
Identification of Autoreactive B Cell Subpopulations in Peripheral Blood of Autoimmune Patients With Pemphigus Vulgaris |
title_sort |
identification of autoreactive b cell subpopulations in peripheral blood of autoimmune patients with pemphigus vulgaris |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2019-06-01 |
description |
Pemphigus vulgaris (PV) is a rare blistering disease caused by IgG autoantibodies against the epidermal adhesion molecules desmoglein (Dsg)3 and Dsg1 providing a well-characterized paradigm of an antibody-mediated organ-specific autoimmune disease. In PV patients who have achieved clinical remission after B cell-depleting therapy, relapses often coincide with a reoccurrence of B cells and Dsg-specific autoantibodies. Here, we analyzed Dsg3-specific B cell subpopulations (i.e., total CD19+ B cells, CD19+CD27−B cells, CD19+CD27+ memory B cells, and CD19+CD27hiCD38hi plasmablasts) in peripheral blood of both PV patients (n = 14) at different stages of disease and healthy individuals (n = 14) by flow cytometry using fluorescently labeled recombinant human Dsg3 protein. Applying this approach, Dsg3-specific B cells could be detected at low frequencies (0.11–0.53% of CD19+ B cells) and numbers of Dsg3-specific memory B cells were significantly increased in PV patients in clinical remission receiving minimal immunosuppressive therapy. Finally, we confirmed in vitro that Dsg3-reactive memory B cells were able to produce anti-Dsg3 IgG autoantibodies upon ex vivo activation. Thus, monitoring of Dsg3-specific B cells in PV is of particular interest to further characterize the immunopathogenesis of PV. |
topic |
autoimmunity pemphigus vulgaris desmoglein 3 B cells flow cytometry |
url |
https://www.frontiersin.org/article/10.3389/fimmu.2019.01375/full |
work_keys_str_mv |
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