Trpv4 Mediates Hypotonic Inhibition of Central Osmosensory Neurons via Taurine Gliotransmission
Summary: The maintenance of hydromineral homeostasis requires bidirectional detection of changes in extracellular fluid osmolality by primary osmosensory neurons (ONs) in the organum vasculosum laminae terminalis (OVLT). Hypertonicity excites ONs in part through the mechanical activation of a varian...
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doaj-31e4f6f7ff2c44a083206c32eddcfd932020-11-24T20:57:17ZengElsevierCell Reports2211-12472018-05-0123822452253Trpv4 Mediates Hypotonic Inhibition of Central Osmosensory Neurons via Taurine GliotransmissionSorana Ciura0Masha Prager-Khoutorsky1Zahra S. Thirouin2Joshua C. Wyrosdic3James E. Olson4Wolfgang Liedtke5Charles W. Bourque6Centre for Research in Neuroscience, Research Institute of the McGill University Health Centre, 1650 Cedar Avenue, Montreal, QC H3G1A4, Canada; Corresponding authorCentre for Research in Neuroscience, Research Institute of the McGill University Health Centre, 1650 Cedar Avenue, Montreal, QC H3G1A4, CanadaCentre for Research in Neuroscience, Research Institute of the McGill University Health Centre, 1650 Cedar Avenue, Montreal, QC H3G1A4, CanadaCentre for Research in Neuroscience, Research Institute of the McGill University Health Centre, 1650 Cedar Avenue, Montreal, QC H3G1A4, CanadaDepartment of Emergency Medicine/Department of Neuroscience, Cell Biology and Physiology, Wright State University, 3640 Colonel Glenn Highway, Dayton, OH 45435, USACentre for Translational Neuroscience, 201G Bryan Research Bldg. Box 2900, Duke University Medical Centre, Durham, NC 27710, USACentre for Research in Neuroscience, Research Institute of the McGill University Health Centre, 1650 Cedar Avenue, Montreal, QC H3G1A4, Canada; Corresponding authorSummary: The maintenance of hydromineral homeostasis requires bidirectional detection of changes in extracellular fluid osmolality by primary osmosensory neurons (ONs) in the organum vasculosum laminae terminalis (OVLT). Hypertonicity excites ONs in part through the mechanical activation of a variant transient receptor potential vanilloid-1 channel (dn-Trpv1). However, the mechanism by which local hypotonicity inhibits ONs in the OVLT remains unknown. Here, we show that hypotonicity can reduce the basal activity of dn-Trpv1 channels and hyperpolarize acutely isolated ONs. Surprisingly, we found that mice lacking dn-Trpv1 maintain normal inhibitory responses to hypotonicity when tested in situ. In the intact setting, hypotonicity inhibits ONs through a non-cell-autonomous mechanism that involves glial release of the glycine receptor agonist taurine through hypotonicity activated anion channels (HAAC) that are activated subsequent to Ca2+ influx through Trpv4 channels. Our study clarifies how Trpv4 channels contribute to the inhibition of OVLT ONs during hypotonicity in situ. : Ciura et al. show that osmosensory neurons in organum vasculosum lamina terminalis are inhibited by hypotonicity. This effect is triggered by activation of Trpv4 channels and Ca2+ accumulation in astrocytes, causing these cells to release taurine through anion channels. Taurine inhibits firing by activating glycine receptors on the osmosensory neurons. Keywords: hyptonicity, taurine, TRPV, osmosensitive, gliotransmission, swellinghttp://www.sciencedirect.com/science/article/pii/S2211124718306685 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sorana Ciura Masha Prager-Khoutorsky Zahra S. Thirouin Joshua C. Wyrosdic James E. Olson Wolfgang Liedtke Charles W. Bourque |
spellingShingle |
Sorana Ciura Masha Prager-Khoutorsky Zahra S. Thirouin Joshua C. Wyrosdic James E. Olson Wolfgang Liedtke Charles W. Bourque Trpv4 Mediates Hypotonic Inhibition of Central Osmosensory Neurons via Taurine Gliotransmission Cell Reports |
author_facet |
Sorana Ciura Masha Prager-Khoutorsky Zahra S. Thirouin Joshua C. Wyrosdic James E. Olson Wolfgang Liedtke Charles W. Bourque |
author_sort |
Sorana Ciura |
title |
Trpv4 Mediates Hypotonic Inhibition of Central Osmosensory Neurons via Taurine Gliotransmission |
title_short |
Trpv4 Mediates Hypotonic Inhibition of Central Osmosensory Neurons via Taurine Gliotransmission |
title_full |
Trpv4 Mediates Hypotonic Inhibition of Central Osmosensory Neurons via Taurine Gliotransmission |
title_fullStr |
Trpv4 Mediates Hypotonic Inhibition of Central Osmosensory Neurons via Taurine Gliotransmission |
title_full_unstemmed |
Trpv4 Mediates Hypotonic Inhibition of Central Osmosensory Neurons via Taurine Gliotransmission |
title_sort |
trpv4 mediates hypotonic inhibition of central osmosensory neurons via taurine gliotransmission |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2018-05-01 |
description |
Summary: The maintenance of hydromineral homeostasis requires bidirectional detection of changes in extracellular fluid osmolality by primary osmosensory neurons (ONs) in the organum vasculosum laminae terminalis (OVLT). Hypertonicity excites ONs in part through the mechanical activation of a variant transient receptor potential vanilloid-1 channel (dn-Trpv1). However, the mechanism by which local hypotonicity inhibits ONs in the OVLT remains unknown. Here, we show that hypotonicity can reduce the basal activity of dn-Trpv1 channels and hyperpolarize acutely isolated ONs. Surprisingly, we found that mice lacking dn-Trpv1 maintain normal inhibitory responses to hypotonicity when tested in situ. In the intact setting, hypotonicity inhibits ONs through a non-cell-autonomous mechanism that involves glial release of the glycine receptor agonist taurine through hypotonicity activated anion channels (HAAC) that are activated subsequent to Ca2+ influx through Trpv4 channels. Our study clarifies how Trpv4 channels contribute to the inhibition of OVLT ONs during hypotonicity in situ. : Ciura et al. show that osmosensory neurons in organum vasculosum lamina terminalis are inhibited by hypotonicity. This effect is triggered by activation of Trpv4 channels and Ca2+ accumulation in astrocytes, causing these cells to release taurine through anion channels. Taurine inhibits firing by activating glycine receptors on the osmosensory neurons. Keywords: hyptonicity, taurine, TRPV, osmosensitive, gliotransmission, swelling |
url |
http://www.sciencedirect.com/science/article/pii/S2211124718306685 |
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