Development and characterization of GR2E Golden rice introgression lines
Abstract Golden Rice with β-carotene in the grain helps to address the problem of vitamin A deficiency. Prior to commercialize Golden Rice, several performance and regulatory checkpoints must be achieved. We report results of marker assisted backcross breeding of the GR2E trait into three popular ri...
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doaj-31f0a0969709448f86f9d24a9861899d2021-01-31T16:25:37ZengNature Publishing GroupScientific Reports2045-23222021-01-0111111210.1038/s41598-021-82001-0Development and characterization of GR2E Golden rice introgression linesB. P. Mallikarjuna Swamy0Severino Marundan1Mercy Samia2Reynante L. Ordonio3Democrito B. Rebong4Ronalyn Miranda5Anielyn Alibuyog6Anna Theresa Rebong7Ma. Angela Tabil8Roel R. Suralta9Antonio A. Alfonso10Partha Sarathi Biswas11Md. Abdul Kader12Russell F. Reinke13Raul Boncodin14Donald J. MacKenzie15International Rice Research Institute (IRRI)International Rice Research Institute (IRRI)International Rice Research Institute (IRRI)Philippines Rice Research Institute (PhilRice)Philippines Rice Research Institute (PhilRice)Philippines Rice Research Institute (PhilRice)Philippines Rice Research Institute (PhilRice)Philippines Rice Research Institute (PhilRice)Philippines Rice Research Institute (PhilRice)Philippines Rice Research Institute (PhilRice)Philippines Rice Research Institute (PhilRice)Plant Breeding Division, Bangladesh Rice Research Institute (BRRI)Plant Breeding Division, Bangladesh Rice Research Institute (BRRI)International Rice Research Institute (IRRI)International Rice Research Institute (IRRI)Institute for International Crop Improvement, Donald Danforth Plant Science CenterAbstract Golden Rice with β-carotene in the grain helps to address the problem of vitamin A deficiency. Prior to commercialize Golden Rice, several performance and regulatory checkpoints must be achieved. We report results of marker assisted backcross breeding of the GR2E trait into three popular rice varieties followed by a series of confined field tests of event GR2E introgression lines to assess their agronomic performance and carotenoid expression. Results from confined tests in the Philippines and Bangladesh have shown that GR2E introgression lines matched the performance of the recurrent parents for agronomic and yield performance, and the key components of grain quality. Moreover, no differences were observed in terms of pest and disease reaction. The best performing lines identified in each genetic background had significant amounts of carotenoids in the milled grains. These lines can supply 30–50% of the estimated average requirements of vitamin A.https://doi.org/10.1038/s41598-021-82001-0 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
B. P. Mallikarjuna Swamy Severino Marundan Mercy Samia Reynante L. Ordonio Democrito B. Rebong Ronalyn Miranda Anielyn Alibuyog Anna Theresa Rebong Ma. Angela Tabil Roel R. Suralta Antonio A. Alfonso Partha Sarathi Biswas Md. Abdul Kader Russell F. Reinke Raul Boncodin Donald J. MacKenzie |
spellingShingle |
B. P. Mallikarjuna Swamy Severino Marundan Mercy Samia Reynante L. Ordonio Democrito B. Rebong Ronalyn Miranda Anielyn Alibuyog Anna Theresa Rebong Ma. Angela Tabil Roel R. Suralta Antonio A. Alfonso Partha Sarathi Biswas Md. Abdul Kader Russell F. Reinke Raul Boncodin Donald J. MacKenzie Development and characterization of GR2E Golden rice introgression lines Scientific Reports |
author_facet |
B. P. Mallikarjuna Swamy Severino Marundan Mercy Samia Reynante L. Ordonio Democrito B. Rebong Ronalyn Miranda Anielyn Alibuyog Anna Theresa Rebong Ma. Angela Tabil Roel R. Suralta Antonio A. Alfonso Partha Sarathi Biswas Md. Abdul Kader Russell F. Reinke Raul Boncodin Donald J. MacKenzie |
author_sort |
B. P. Mallikarjuna Swamy |
title |
Development and characterization of GR2E Golden rice introgression lines |
title_short |
Development and characterization of GR2E Golden rice introgression lines |
title_full |
Development and characterization of GR2E Golden rice introgression lines |
title_fullStr |
Development and characterization of GR2E Golden rice introgression lines |
title_full_unstemmed |
Development and characterization of GR2E Golden rice introgression lines |
title_sort |
development and characterization of gr2e golden rice introgression lines |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-01-01 |
description |
Abstract Golden Rice with β-carotene in the grain helps to address the problem of vitamin A deficiency. Prior to commercialize Golden Rice, several performance and regulatory checkpoints must be achieved. We report results of marker assisted backcross breeding of the GR2E trait into three popular rice varieties followed by a series of confined field tests of event GR2E introgression lines to assess their agronomic performance and carotenoid expression. Results from confined tests in the Philippines and Bangladesh have shown that GR2E introgression lines matched the performance of the recurrent parents for agronomic and yield performance, and the key components of grain quality. Moreover, no differences were observed in terms of pest and disease reaction. The best performing lines identified in each genetic background had significant amounts of carotenoids in the milled grains. These lines can supply 30–50% of the estimated average requirements of vitamin A. |
url |
https://doi.org/10.1038/s41598-021-82001-0 |
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