Integrating miRNA and mRNA Expression Profiling Uncovers miRNAs Underlying Fat Deposition in Sheep
MicroRNAs (miRNAs) are endogenous, noncoding RNAs that regulate various biological processes including adipogenesis and fat metabolism. Here, we adopted a deep sequencing approach to determine the identity and abundance of miRNAs involved in fat deposition in adipose tissues from fat-tailed (Kazakhs...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2017-01-01
|
Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2017/1857580 |
id |
doaj-31f35eed81474de4ada899508b9b216f |
---|---|
record_format |
Article |
spelling |
doaj-31f35eed81474de4ada899508b9b216f2020-11-24T23:04:17ZengHindawi LimitedBioMed Research International2314-61332314-61412017-01-01201710.1155/2017/18575801857580Integrating miRNA and mRNA Expression Profiling Uncovers miRNAs Underlying Fat Deposition in SheepGuangxian Zhou0Xiaolong Wang1Chao Yuan2Danju Kang3Xiaochun Xu4Jiping Zhou5Rongqing Geng6Yuxin Yang7Zhaoxia Yang8Yulin Chen9College of Animal Science and Technology, Northwest A&F University, Yangling, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, ChinaQinghai Academy of Animal Science and Veterinary Medicine, Xining, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling, ChinaMicroRNAs (miRNAs) are endogenous, noncoding RNAs that regulate various biological processes including adipogenesis and fat metabolism. Here, we adopted a deep sequencing approach to determine the identity and abundance of miRNAs involved in fat deposition in adipose tissues from fat-tailed (Kazakhstan sheep, KS) and thin-tailed (Tibetan sheep, TS) sheep breeds. By comparing HiSeq data of these two breeds, 539 miRNAs were shared in both breeds, whereas 179 and 97 miRNAs were uniquely expressed in KS and TS, respectively. We also identified 35 miRNAs that are considered to be putative novel miRNAs. The integration of miRNA-mRNA analysis revealed that miRNA-associated targets were mainly involved in the gene ontology (GO) biological processes concerning cellular process and metabolic process, and miRNAs play critical roles in fat deposition through their ability to regulate fundamental pathways. These pathways included the MAPK signaling pathway, FoxO and Wnt signaling pathway, and focal adhesion. Taken together, our results define miRNA expression signatures that may contribute to fat deposition and lipid metabolism in sheep.http://dx.doi.org/10.1155/2017/1857580 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guangxian Zhou Xiaolong Wang Chao Yuan Danju Kang Xiaochun Xu Jiping Zhou Rongqing Geng Yuxin Yang Zhaoxia Yang Yulin Chen |
spellingShingle |
Guangxian Zhou Xiaolong Wang Chao Yuan Danju Kang Xiaochun Xu Jiping Zhou Rongqing Geng Yuxin Yang Zhaoxia Yang Yulin Chen Integrating miRNA and mRNA Expression Profiling Uncovers miRNAs Underlying Fat Deposition in Sheep BioMed Research International |
author_facet |
Guangxian Zhou Xiaolong Wang Chao Yuan Danju Kang Xiaochun Xu Jiping Zhou Rongqing Geng Yuxin Yang Zhaoxia Yang Yulin Chen |
author_sort |
Guangxian Zhou |
title |
Integrating miRNA and mRNA Expression Profiling Uncovers miRNAs Underlying Fat Deposition in Sheep |
title_short |
Integrating miRNA and mRNA Expression Profiling Uncovers miRNAs Underlying Fat Deposition in Sheep |
title_full |
Integrating miRNA and mRNA Expression Profiling Uncovers miRNAs Underlying Fat Deposition in Sheep |
title_fullStr |
Integrating miRNA and mRNA Expression Profiling Uncovers miRNAs Underlying Fat Deposition in Sheep |
title_full_unstemmed |
Integrating miRNA and mRNA Expression Profiling Uncovers miRNAs Underlying Fat Deposition in Sheep |
title_sort |
integrating mirna and mrna expression profiling uncovers mirnas underlying fat deposition in sheep |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2017-01-01 |
description |
MicroRNAs (miRNAs) are endogenous, noncoding RNAs that regulate various biological processes including adipogenesis and fat metabolism. Here, we adopted a deep sequencing approach to determine the identity and abundance of miRNAs involved in fat deposition in adipose tissues from fat-tailed (Kazakhstan sheep, KS) and thin-tailed (Tibetan sheep, TS) sheep breeds. By comparing HiSeq data of these two breeds, 539 miRNAs were shared in both breeds, whereas 179 and 97 miRNAs were uniquely expressed in KS and TS, respectively. We also identified 35 miRNAs that are considered to be putative novel miRNAs. The integration of miRNA-mRNA analysis revealed that miRNA-associated targets were mainly involved in the gene ontology (GO) biological processes concerning cellular process and metabolic process, and miRNAs play critical roles in fat deposition through their ability to regulate fundamental pathways. These pathways included the MAPK signaling pathway, FoxO and Wnt signaling pathway, and focal adhesion. Taken together, our results define miRNA expression signatures that may contribute to fat deposition and lipid metabolism in sheep. |
url |
http://dx.doi.org/10.1155/2017/1857580 |
work_keys_str_mv |
AT guangxianzhou integratingmirnaandmrnaexpressionprofilinguncoversmirnasunderlyingfatdepositioninsheep AT xiaolongwang integratingmirnaandmrnaexpressionprofilinguncoversmirnasunderlyingfatdepositioninsheep AT chaoyuan integratingmirnaandmrnaexpressionprofilinguncoversmirnasunderlyingfatdepositioninsheep AT danjukang integratingmirnaandmrnaexpressionprofilinguncoversmirnasunderlyingfatdepositioninsheep AT xiaochunxu integratingmirnaandmrnaexpressionprofilinguncoversmirnasunderlyingfatdepositioninsheep AT jipingzhou integratingmirnaandmrnaexpressionprofilinguncoversmirnasunderlyingfatdepositioninsheep AT rongqinggeng integratingmirnaandmrnaexpressionprofilinguncoversmirnasunderlyingfatdepositioninsheep AT yuxinyang integratingmirnaandmrnaexpressionprofilinguncoversmirnasunderlyingfatdepositioninsheep AT zhaoxiayang integratingmirnaandmrnaexpressionprofilinguncoversmirnasunderlyingfatdepositioninsheep AT yulinchen integratingmirnaandmrnaexpressionprofilinguncoversmirnasunderlyingfatdepositioninsheep |
_version_ |
1725631536707928064 |