Development of a Rapid Cell-free Method for Cytotoxicity Assessment of Vapor Phase of Cigarette Smoke
Currently, several in vitro tests are widely used to measure toxicological properties of mainstream smoke (Neutral Red Uptake Assay, Micronucleus assay, Ames Test). These tests are necessary to assess cytotoxicity, genotoxicity, and mutagenicity, but are time consuming. This is essentially due to th...
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doaj-321140395f294ef2b92aace4e003e1052020-11-24T22:53:29ZengSciendoBeiträge zur Tabakforschung International1612-92372014-12-0124415716510.2478/cttr-2013-0893cttr-2013-0893Development of a Rapid Cell-free Method for Cytotoxicity Assessment of Vapor Phase of Cigarette SmokeCahours X0Blanchet M1Dubois M2Haond C3SEITA, Imperial Tobacco Group, 4, rue Andre Dessaux, 45404 Fleury-les-Aubrais, FranceSEITA, Imperial Tobacco Group, 4, rue Andre Dessaux, 45404 Fleury-les-Aubrais, FranceSEITA, Imperial Tobacco Group, 4, rue Andre Dessaux, 45404 Fleury-les-Aubrais, FranceSEITA, Imperial Tobacco Group, 4, rue Andre Dessaux, 45404 Fleury-les-Aubrais, FranceCurrently, several in vitro tests are widely used to measure toxicological properties of mainstream smoke (Neutral Red Uptake Assay, Micronucleus assay, Ames Test). These tests are necessary to assess cytotoxicity, genotoxicity, and mutagenicity, but are time consuming. This is essentially due to the preparation and the handling of cells. It is difficult to use these in vitro tests as screening method for product testing and development. For a better assessment of the cytotoxicity of the vapor phase, a rapid cell-free method has been developed. This paper describes a capillary electrophoresis cell-free method, based on the depletion of an anti-oxidant L-gamma-glutamyl-L-cysteinylglycine (GSH), applied to an aliquot of vapor phase phosphate buffered saline (PBS)-trapped cigarette smoke (as recommended for in vitro testing). The correlation between this method and the survival/viability test (Neutral Red cytotoxicity) is excellent (coefficient of correlation (r = 0.99)).http://www.degruyter.com/view/j/cttr.2011.24.issue-4/cttr-2013-0893/cttr-2013-0893.xml?format=INT |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cahours X Blanchet M Dubois M Haond C |
spellingShingle |
Cahours X Blanchet M Dubois M Haond C Development of a Rapid Cell-free Method for Cytotoxicity Assessment of Vapor Phase of Cigarette Smoke Beiträge zur Tabakforschung International |
author_facet |
Cahours X Blanchet M Dubois M Haond C |
author_sort |
Cahours X |
title |
Development of a Rapid Cell-free Method for Cytotoxicity Assessment of Vapor Phase of Cigarette Smoke |
title_short |
Development of a Rapid Cell-free Method for Cytotoxicity Assessment of Vapor Phase of Cigarette Smoke |
title_full |
Development of a Rapid Cell-free Method for Cytotoxicity Assessment of Vapor Phase of Cigarette Smoke |
title_fullStr |
Development of a Rapid Cell-free Method for Cytotoxicity Assessment of Vapor Phase of Cigarette Smoke |
title_full_unstemmed |
Development of a Rapid Cell-free Method for Cytotoxicity Assessment of Vapor Phase of Cigarette Smoke |
title_sort |
development of a rapid cell-free method for cytotoxicity assessment of vapor phase of cigarette smoke |
publisher |
Sciendo |
series |
Beiträge zur Tabakforschung International |
issn |
1612-9237 |
publishDate |
2014-12-01 |
description |
Currently, several in vitro tests are widely used to measure toxicological properties of mainstream smoke (Neutral Red Uptake Assay, Micronucleus assay, Ames Test). These tests are necessary to assess cytotoxicity, genotoxicity, and mutagenicity, but are time consuming. This is essentially due to the preparation and the handling of cells. It is difficult to use these in vitro tests as screening method for product testing and development. For a better assessment of the cytotoxicity of the vapor phase, a rapid cell-free method has been developed. This paper describes a capillary electrophoresis cell-free method, based on the depletion of an anti-oxidant L-gamma-glutamyl-L-cysteinylglycine (GSH), applied to an aliquot of vapor phase phosphate buffered saline (PBS)-trapped cigarette smoke (as recommended for in vitro testing). The correlation between this method and the survival/viability test (Neutral Red cytotoxicity) is excellent (coefficient of correlation (r = 0.99)). |
url |
http://www.degruyter.com/view/j/cttr.2011.24.issue-4/cttr-2013-0893/cttr-2013-0893.xml?format=INT |
work_keys_str_mv |
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