Adipocyte abundances of CES1, CRYAB, ENO1 and GANAB are modified in-vitro by glucose restriction and are associated with cellular remodelling during weight regain
Long-term weight loss maintenance is a problem of overweight and obesity. Changes of gene expression during weight loss (WL) by calorie restriction (CR) are linked to the risk of weight regain (WR). However, detailed information on genes/proteins involved in the mechanism is still lacking. Therefore...
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doaj-3227fb21dd4043de9b601f668f675ab82020-11-25T02:45:39ZengTaylor & Francis GroupAdipocyte2162-39452162-397X2019-01-018119020010.1080/21623945.2019.16087571608757Adipocyte abundances of CES1, CRYAB, ENO1 and GANAB are modified in-vitro by glucose restriction and are associated with cellular remodelling during weight regainQi Qiao0Freek G. Bouwman1Marleen A. van Baak2Nadia J. T. Roumans3Roel G. Vink4Susan L. M. Coort5Johan W. Renes6Edwin C. M. Mariman7Maastricht University Medical CentreMaastricht University Medical CentreMaastricht University Medical CentreMaastricht University Medical CentreMaastricht University Medical CentreMaastricht University Medical CentreMaastricht University Medical CentreMaastricht University Medical CentreLong-term weight loss maintenance is a problem of overweight and obesity. Changes of gene expression during weight loss (WL) by calorie restriction (CR) are linked to the risk of weight regain (WR). However, detailed information on genes/proteins involved in the mechanism is still lacking. Therefore, we developed an in-vitro model system for glucose restriction (GR) and refeeding (RF) to uncover proteome differences between GR with RF vs normal feeding, of which we explored the relation with WR after WL. Human Simpson-Golabi-Behmel Syndrome cells were subjected to changing levels of glucose to mimic the condition of CR and RF. Proteome profiling was performed by liquid chromatography tandem mass spectrometry. This in-vitro model revealed 44 proteins differentially expressed after GR and RF versus feeding including proteins of the focal adhesions. Four proteins showed a persistent up- or down-regulation: liver carboxylesterase (CES1), mitochondrial superoxide dismutase [Mn] (SOD2), alpha-crystallin B-chain (CRYAB), alpha-enolase (ENO1). In-vivo weight loss-induced RNA expression changes linked CES1, CRYAB and ENO1 to WR. Moreover, of these 44 proteins, CES1 and glucosidase II alpha subunit (GANAB) during follow up correlated with WR. Correlation clustering of in-vivo protein expression data indicated an interaction of these proteins with structural components of the focal adhesions and cytoplasmic filaments in the adipocytes.http://dx.doi.org/10.1080/21623945.2019.1608757in-vitro fat-regainsgbs adipocytesproteomicsweight regainfocal adhesion |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qi Qiao Freek G. Bouwman Marleen A. van Baak Nadia J. T. Roumans Roel G. Vink Susan L. M. Coort Johan W. Renes Edwin C. M. Mariman |
spellingShingle |
Qi Qiao Freek G. Bouwman Marleen A. van Baak Nadia J. T. Roumans Roel G. Vink Susan L. M. Coort Johan W. Renes Edwin C. M. Mariman Adipocyte abundances of CES1, CRYAB, ENO1 and GANAB are modified in-vitro by glucose restriction and are associated with cellular remodelling during weight regain Adipocyte in-vitro fat-regain sgbs adipocytes proteomics weight regain focal adhesion |
author_facet |
Qi Qiao Freek G. Bouwman Marleen A. van Baak Nadia J. T. Roumans Roel G. Vink Susan L. M. Coort Johan W. Renes Edwin C. M. Mariman |
author_sort |
Qi Qiao |
title |
Adipocyte abundances of CES1, CRYAB, ENO1 and GANAB are modified in-vitro by glucose restriction and are associated with cellular remodelling during weight regain |
title_short |
Adipocyte abundances of CES1, CRYAB, ENO1 and GANAB are modified in-vitro by glucose restriction and are associated with cellular remodelling during weight regain |
title_full |
Adipocyte abundances of CES1, CRYAB, ENO1 and GANAB are modified in-vitro by glucose restriction and are associated with cellular remodelling during weight regain |
title_fullStr |
Adipocyte abundances of CES1, CRYAB, ENO1 and GANAB are modified in-vitro by glucose restriction and are associated with cellular remodelling during weight regain |
title_full_unstemmed |
Adipocyte abundances of CES1, CRYAB, ENO1 and GANAB are modified in-vitro by glucose restriction and are associated with cellular remodelling during weight regain |
title_sort |
adipocyte abundances of ces1, cryab, eno1 and ganab are modified in-vitro by glucose restriction and are associated with cellular remodelling during weight regain |
publisher |
Taylor & Francis Group |
series |
Adipocyte |
issn |
2162-3945 2162-397X |
publishDate |
2019-01-01 |
description |
Long-term weight loss maintenance is a problem of overweight and obesity. Changes of gene expression during weight loss (WL) by calorie restriction (CR) are linked to the risk of weight regain (WR). However, detailed information on genes/proteins involved in the mechanism is still lacking. Therefore, we developed an in-vitro model system for glucose restriction (GR) and refeeding (RF) to uncover proteome differences between GR with RF vs normal feeding, of which we explored the relation with WR after WL. Human Simpson-Golabi-Behmel Syndrome cells were subjected to changing levels of glucose to mimic the condition of CR and RF. Proteome profiling was performed by liquid chromatography tandem mass spectrometry. This in-vitro model revealed 44 proteins differentially expressed after GR and RF versus feeding including proteins of the focal adhesions. Four proteins showed a persistent up- or down-regulation: liver carboxylesterase (CES1), mitochondrial superoxide dismutase [Mn] (SOD2), alpha-crystallin B-chain (CRYAB), alpha-enolase (ENO1). In-vivo weight loss-induced RNA expression changes linked CES1, CRYAB and ENO1 to WR. Moreover, of these 44 proteins, CES1 and glucosidase II alpha subunit (GANAB) during follow up correlated with WR. Correlation clustering of in-vivo protein expression data indicated an interaction of these proteins with structural components of the focal adhesions and cytoplasmic filaments in the adipocytes. |
topic |
in-vitro fat-regain sgbs adipocytes proteomics weight regain focal adhesion |
url |
http://dx.doi.org/10.1080/21623945.2019.1608757 |
work_keys_str_mv |
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