The Suppression of Th1 Response by Inducing TGF-β1 From Regulatory T Cells in Bovine Mycoplasmosis
Regulatory T cells (Tregs) regulate immune responses and maintain host immune homeostasis. Tregs contribute to the disease progression of several chronic infections by oversuppressing immune responses via the secretion of immunosuppressive cytokines, such as transforming growth factor (TGF)-β and in...
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2020-12-01
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doaj-322f6ea3de6c4d969d146127ca9fc2e92020-12-08T08:37:05ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692020-12-01710.3389/fvets.2020.609443609443The Suppression of Th1 Response by Inducing TGF-β1 From Regulatory T Cells in Bovine MycoplasmosisYamato Sajiki0Satoru Konnai1Satoru Konnai2Shinya Goto3Tomohiro Okagawa4Kosuke Ohira5Honami Shimakura6Naoya Maekawa7Satoshi Gondaira8Hidetoshi Higuchi9Motoshi Tajima10Yuki Hirano11Junko Kohara12Shiro Murata13Shiro Murata14Kazuhiko Ohashi15Kazuhiko Ohashi16Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanDepartment of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanDepartment of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanDepartment of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanDepartment of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanDepartment of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanDepartment of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanDepartment of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanSchool of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, JapanSchool of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, JapanSchool of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, JapanAnimal Research Center, Agriculture Research Department, Hokkaido Research Organization, Shintoku, JapanAnimal Research Center, Agriculture Research Department, Hokkaido Research Organization, Shintoku, JapanDepartment of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanDepartment of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanDepartment of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanDepartment of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, JapanRegulatory T cells (Tregs) regulate immune responses and maintain host immune homeostasis. Tregs contribute to the disease progression of several chronic infections by oversuppressing immune responses via the secretion of immunosuppressive cytokines, such as transforming growth factor (TGF)-β and interleukin-10. In the present study, we examined the association of Tregs with Mycoplasma bovis infection, in which immunosuppression is frequently observed. Compared with uninfected cattle, the percentage of Tregs, CD4+CD25highFoxp3+ T cells, was increased in M. bovis-infected cattle. Additionally, the plasma of M. bovis-infected cattle contained the high concentrations of TGF-β1, and M. bovis infection induced TGF-β1 production from bovine immune cells in in vitro cultures. Finally, we analyzed the immunosuppressive effects of TGF-β1 on bovine immune cells. Treatment with TGF-β1 significantly decreased the expression of CD69, an activation marker, in T cells, and Th1 cytokine production in vitro. These results suggest that the increase in Tregs and TGF-β1 secretion could be one of the immunosuppressive mechanisms and that lead to increased susceptibility to other infections in terms of exacerbation of disease during M. bovis infection.https://www.frontiersin.org/articles/10.3389/fvets.2020.609443/fullTGF-β1Mycoplasma bovisregulatory T cellimmunosuppressioncattle |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yamato Sajiki Satoru Konnai Satoru Konnai Shinya Goto Tomohiro Okagawa Kosuke Ohira Honami Shimakura Naoya Maekawa Satoshi Gondaira Hidetoshi Higuchi Motoshi Tajima Yuki Hirano Junko Kohara Shiro Murata Shiro Murata Kazuhiko Ohashi Kazuhiko Ohashi |
spellingShingle |
Yamato Sajiki Satoru Konnai Satoru Konnai Shinya Goto Tomohiro Okagawa Kosuke Ohira Honami Shimakura Naoya Maekawa Satoshi Gondaira Hidetoshi Higuchi Motoshi Tajima Yuki Hirano Junko Kohara Shiro Murata Shiro Murata Kazuhiko Ohashi Kazuhiko Ohashi The Suppression of Th1 Response by Inducing TGF-β1 From Regulatory T Cells in Bovine Mycoplasmosis Frontiers in Veterinary Science TGF-β1 Mycoplasma bovis regulatory T cell immunosuppression cattle |
author_facet |
Yamato Sajiki Satoru Konnai Satoru Konnai Shinya Goto Tomohiro Okagawa Kosuke Ohira Honami Shimakura Naoya Maekawa Satoshi Gondaira Hidetoshi Higuchi Motoshi Tajima Yuki Hirano Junko Kohara Shiro Murata Shiro Murata Kazuhiko Ohashi Kazuhiko Ohashi |
author_sort |
Yamato Sajiki |
title |
The Suppression of Th1 Response by Inducing TGF-β1 From Regulatory T Cells in Bovine Mycoplasmosis |
title_short |
The Suppression of Th1 Response by Inducing TGF-β1 From Regulatory T Cells in Bovine Mycoplasmosis |
title_full |
The Suppression of Th1 Response by Inducing TGF-β1 From Regulatory T Cells in Bovine Mycoplasmosis |
title_fullStr |
The Suppression of Th1 Response by Inducing TGF-β1 From Regulatory T Cells in Bovine Mycoplasmosis |
title_full_unstemmed |
The Suppression of Th1 Response by Inducing TGF-β1 From Regulatory T Cells in Bovine Mycoplasmosis |
title_sort |
suppression of th1 response by inducing tgf-β1 from regulatory t cells in bovine mycoplasmosis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Veterinary Science |
issn |
2297-1769 |
publishDate |
2020-12-01 |
description |
Regulatory T cells (Tregs) regulate immune responses and maintain host immune homeostasis. Tregs contribute to the disease progression of several chronic infections by oversuppressing immune responses via the secretion of immunosuppressive cytokines, such as transforming growth factor (TGF)-β and interleukin-10. In the present study, we examined the association of Tregs with Mycoplasma bovis infection, in which immunosuppression is frequently observed. Compared with uninfected cattle, the percentage of Tregs, CD4+CD25highFoxp3+ T cells, was increased in M. bovis-infected cattle. Additionally, the plasma of M. bovis-infected cattle contained the high concentrations of TGF-β1, and M. bovis infection induced TGF-β1 production from bovine immune cells in in vitro cultures. Finally, we analyzed the immunosuppressive effects of TGF-β1 on bovine immune cells. Treatment with TGF-β1 significantly decreased the expression of CD69, an activation marker, in T cells, and Th1 cytokine production in vitro. These results suggest that the increase in Tregs and TGF-β1 secretion could be one of the immunosuppressive mechanisms and that lead to increased susceptibility to other infections in terms of exacerbation of disease during M. bovis infection. |
topic |
TGF-β1 Mycoplasma bovis regulatory T cell immunosuppression cattle |
url |
https://www.frontiersin.org/articles/10.3389/fvets.2020.609443/full |
work_keys_str_mv |
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