Assay of antioxidant potential of two Aspergillus isolates by different methods under various physio-chemical conditions
The objective of this work was to screen fungi isolated from soil of different areas of Punjab, India for antioxidant activity by dot blot assay and around 45% of fungal isolates demonstrated antioxidant potential. Two selected strains of Aspergillus spp (Aspergillus PR78 and Aspergillus PR66) showi...
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Sociedade Brasileira de Microbiologia
2010-10-01
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doaj-323bf8d4251e4373b9927c934bd66b332020-11-24T21:28:16ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1517-83821678-44052010-10-0141376577710.1590/S1517-83822010000300029Assay of antioxidant potential of two Aspergillus isolates by different methods under various physio-chemical conditionsDaljit Singh AroraPriyanka ChandraThe objective of this work was to screen fungi isolated from soil of different areas of Punjab, India for antioxidant activity by dot blot assay and around 45% of fungal isolates demonstrated antioxidant potential. Two selected strains of Aspergillus spp (Aspergillus PR78 and Aspergillus PR66) showing quantitatively best antioxidant activity by DPPH assay were further tested for their reducing power, ferrous ion and nitric oxide ion scavenging activity, FRAP assay and total phenolic content. Different physio-chemical parameters were optimized for enhancement of the activity. This revealed stationary culture grown for 10 days at 25ºC at pH 7 to be the best for antioxidant activity. Sucrose in the medium as carbon source resulted in highest antioxidant activity. Sodium nitrate, yeast extract, and peptone were good sources of nitrogen but sodium nitrate was the best among these. The extraction of the broth culture filtrates with different solvents revealed ethyl acetate extract to possess the best antioxidant activity. The activity as expressed by ethyl acetate extract of Aspergillus PR78 was equally effective as that of commonly used antioxidant standard, ascorbic acid.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300029Antioxidant potentialAspergillusDot blot assayDPPH assayFRAP assayReducing powerSoil fungi |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Daljit Singh Arora Priyanka Chandra |
spellingShingle |
Daljit Singh Arora Priyanka Chandra Assay of antioxidant potential of two Aspergillus isolates by different methods under various physio-chemical conditions Brazilian Journal of Microbiology Antioxidant potential Aspergillus Dot blot assay DPPH assay FRAP assay Reducing power Soil fungi |
author_facet |
Daljit Singh Arora Priyanka Chandra |
author_sort |
Daljit Singh Arora |
title |
Assay of antioxidant potential of two Aspergillus isolates by different methods under various physio-chemical conditions |
title_short |
Assay of antioxidant potential of two Aspergillus isolates by different methods under various physio-chemical conditions |
title_full |
Assay of antioxidant potential of two Aspergillus isolates by different methods under various physio-chemical conditions |
title_fullStr |
Assay of antioxidant potential of two Aspergillus isolates by different methods under various physio-chemical conditions |
title_full_unstemmed |
Assay of antioxidant potential of two Aspergillus isolates by different methods under various physio-chemical conditions |
title_sort |
assay of antioxidant potential of two aspergillus isolates by different methods under various physio-chemical conditions |
publisher |
Sociedade Brasileira de Microbiologia |
series |
Brazilian Journal of Microbiology |
issn |
1517-8382 1678-4405 |
publishDate |
2010-10-01 |
description |
The objective of this work was to screen fungi isolated from soil of different areas of Punjab, India for antioxidant activity by dot blot assay and around 45% of fungal isolates demonstrated antioxidant potential. Two selected strains of Aspergillus spp (Aspergillus PR78 and Aspergillus PR66) showing quantitatively best antioxidant activity by DPPH assay were further tested for their reducing power, ferrous ion and nitric oxide ion scavenging activity, FRAP assay and total phenolic content. Different physio-chemical parameters were optimized for enhancement of the activity. This revealed stationary culture grown for 10 days at 25ºC at pH 7 to be the best for antioxidant activity. Sucrose in the medium as carbon source resulted in highest antioxidant activity. Sodium nitrate, yeast extract, and peptone were good sources of nitrogen but sodium nitrate was the best among these. The extraction of the broth culture filtrates with different solvents revealed ethyl acetate extract to possess the best antioxidant activity. The activity as expressed by ethyl acetate extract of Aspergillus PR78 was equally effective as that of commonly used antioxidant standard, ascorbic acid. |
topic |
Antioxidant potential Aspergillus Dot blot assay DPPH assay FRAP assay Reducing power Soil fungi |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300029 |
work_keys_str_mv |
AT daljitsingharora assayofantioxidantpotentialoftwoaspergillusisolatesbydifferentmethodsundervariousphysiochemicalconditions AT priyankachandra assayofantioxidantpotentialoftwoaspergillusisolatesbydifferentmethodsundervariousphysiochemicalconditions |
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