Quantitative characterization of all single amino acid variants of a viral capsid-based drug delivery vehicle
Self-assembling proteins have important applications in industrial technologies, but it is difficult to predict how mutations affect assembly. Here the authors present SyMAPS, coupling comprehensive codon mutagenesis with high-throughput sequencing, and apply it to bacteriophage capsid protein.
Main Authors: | Emily C. Hartman, Christopher M. Jakobson, Andrew H. Favor, Marco J. Lobba, Ester Álvarez-Benedicto, Matthew B. Francis, Danielle Tullman-Ercek |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2018-04-01
|
Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-018-03783-y |
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