Genome divergence and increased virulence of outbreak associated Salmonella enterica subspecies enterica serovar Heidelberg
Abstract Salmonella enterica serotype Heidelberg is primarily a poultry adapted serotype of Salmonella that can also colonize other hosts and cause human disease. In this study, we compared the genomes of outbreak associated non-outbreak causing Salmonella ser. Heidelberg strains from diverse hosts...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2018-12-01
|
Series: | Gut Pathogens |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s13099-018-0279-0 |
id |
doaj-328bf473d1244bb899b647f5c44a544e |
---|---|
record_format |
Article |
spelling |
doaj-328bf473d1244bb899b647f5c44a544e2020-11-25T00:45:35ZengBMCGut Pathogens1757-47492018-12-011011910.1186/s13099-018-0279-0Genome divergence and increased virulence of outbreak associated Salmonella enterica subspecies enterica serovar HeidelbergLinto Antony0Melissa Behr1Donald Sockett2Dale Miskimins3Nicole Aulik4Jane Christopher-Hennings5Eric Nelson6Marc W. Allard7Joy Scaria8Department of Veterinary and Biomedical Sciences, South Dakota State UniversityDepartment of Veterinary and Biomedical Sciences, South Dakota State UniversityWisconsin Veterinary Diagnostic Laboratory, University of WisconsinDepartment of Veterinary and Biomedical Sciences, South Dakota State UniversityWisconsin Veterinary Diagnostic Laboratory, University of WisconsinDepartment of Veterinary and Biomedical Sciences, South Dakota State UniversityDepartment of Veterinary and Biomedical Sciences, South Dakota State UniversityDivision of Microbiology, Office of Regulatory Science, Center for Food Safety and Nutrition, U.S. Food and Drug AdministrationDepartment of Veterinary and Biomedical Sciences, South Dakota State UniversityAbstract Salmonella enterica serotype Heidelberg is primarily a poultry adapted serotype of Salmonella that can also colonize other hosts and cause human disease. In this study, we compared the genomes of outbreak associated non-outbreak causing Salmonella ser. Heidelberg strains from diverse hosts and geographical regions. Human outbreak associated strains in this study were from a 2015 multistate outbreak of Salmonella ser. Heidelberg involving 15 states in the United States which originated from bull calves. Our clinicopathologic examination revealed that cases involving Salmonella ser. Heidelberg strains were predominantly young, less than weeks-old, dairy calves. Pre-existing or concurrent disease was found in the majority of the calves. Detection of Salmonella ser. Heidelberg correlated with markedly increased death losses clinically comparable to those seen in herds infected with S. Dublin, a known serious pathogen of cattle. Whole genome based single nucleotide polymorphism based analysis revealed that these calf isolates formed a distinct cluster along with outbreak associated human isolates. The defining feature of the outbreak associated strains, when compared to older isolates of S. Heidelberg, is that all isolates in this cluster contained Saf fimbrial genes which are generally absent in S. Heidelberg. The acquisition of several single nucleotide polymorphisms and the gain of Saf fimbrial genes may have contributed to the increased disease severity of these Salmonella ser. Heidelberg strains.http://link.springer.com/article/10.1186/s13099-018-0279-0Salmonella HeidelbergOutbreakGenomic epidemiologySNPFimbrial geneAdhesion |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Linto Antony Melissa Behr Donald Sockett Dale Miskimins Nicole Aulik Jane Christopher-Hennings Eric Nelson Marc W. Allard Joy Scaria |
spellingShingle |
Linto Antony Melissa Behr Donald Sockett Dale Miskimins Nicole Aulik Jane Christopher-Hennings Eric Nelson Marc W. Allard Joy Scaria Genome divergence and increased virulence of outbreak associated Salmonella enterica subspecies enterica serovar Heidelberg Gut Pathogens Salmonella Heidelberg Outbreak Genomic epidemiology SNP Fimbrial gene Adhesion |
author_facet |
Linto Antony Melissa Behr Donald Sockett Dale Miskimins Nicole Aulik Jane Christopher-Hennings Eric Nelson Marc W. Allard Joy Scaria |
author_sort |
Linto Antony |
title |
Genome divergence and increased virulence of outbreak associated Salmonella enterica subspecies enterica serovar Heidelberg |
title_short |
Genome divergence and increased virulence of outbreak associated Salmonella enterica subspecies enterica serovar Heidelberg |
title_full |
Genome divergence and increased virulence of outbreak associated Salmonella enterica subspecies enterica serovar Heidelberg |
title_fullStr |
Genome divergence and increased virulence of outbreak associated Salmonella enterica subspecies enterica serovar Heidelberg |
title_full_unstemmed |
Genome divergence and increased virulence of outbreak associated Salmonella enterica subspecies enterica serovar Heidelberg |
title_sort |
genome divergence and increased virulence of outbreak associated salmonella enterica subspecies enterica serovar heidelberg |
publisher |
BMC |
series |
Gut Pathogens |
issn |
1757-4749 |
publishDate |
2018-12-01 |
description |
Abstract Salmonella enterica serotype Heidelberg is primarily a poultry adapted serotype of Salmonella that can also colonize other hosts and cause human disease. In this study, we compared the genomes of outbreak associated non-outbreak causing Salmonella ser. Heidelberg strains from diverse hosts and geographical regions. Human outbreak associated strains in this study were from a 2015 multistate outbreak of Salmonella ser. Heidelberg involving 15 states in the United States which originated from bull calves. Our clinicopathologic examination revealed that cases involving Salmonella ser. Heidelberg strains were predominantly young, less than weeks-old, dairy calves. Pre-existing or concurrent disease was found in the majority of the calves. Detection of Salmonella ser. Heidelberg correlated with markedly increased death losses clinically comparable to those seen in herds infected with S. Dublin, a known serious pathogen of cattle. Whole genome based single nucleotide polymorphism based analysis revealed that these calf isolates formed a distinct cluster along with outbreak associated human isolates. The defining feature of the outbreak associated strains, when compared to older isolates of S. Heidelberg, is that all isolates in this cluster contained Saf fimbrial genes which are generally absent in S. Heidelberg. The acquisition of several single nucleotide polymorphisms and the gain of Saf fimbrial genes may have contributed to the increased disease severity of these Salmonella ser. Heidelberg strains. |
topic |
Salmonella Heidelberg Outbreak Genomic epidemiology SNP Fimbrial gene Adhesion |
url |
http://link.springer.com/article/10.1186/s13099-018-0279-0 |
work_keys_str_mv |
AT lintoantony genomedivergenceandincreasedvirulenceofoutbreakassociatedsalmonellaentericasubspeciesentericaserovarheidelberg AT melissabehr genomedivergenceandincreasedvirulenceofoutbreakassociatedsalmonellaentericasubspeciesentericaserovarheidelberg AT donaldsockett genomedivergenceandincreasedvirulenceofoutbreakassociatedsalmonellaentericasubspeciesentericaserovarheidelberg AT dalemiskimins genomedivergenceandincreasedvirulenceofoutbreakassociatedsalmonellaentericasubspeciesentericaserovarheidelberg AT nicoleaulik genomedivergenceandincreasedvirulenceofoutbreakassociatedsalmonellaentericasubspeciesentericaserovarheidelberg AT janechristopherhennings genomedivergenceandincreasedvirulenceofoutbreakassociatedsalmonellaentericasubspeciesentericaserovarheidelberg AT ericnelson genomedivergenceandincreasedvirulenceofoutbreakassociatedsalmonellaentericasubspeciesentericaserovarheidelberg AT marcwallard genomedivergenceandincreasedvirulenceofoutbreakassociatedsalmonellaentericasubspeciesentericaserovarheidelberg AT joyscaria genomedivergenceandincreasedvirulenceofoutbreakassociatedsalmonellaentericasubspeciesentericaserovarheidelberg |
_version_ |
1725269428404224000 |