Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon Resonance
The applications of pharmaceutical and medical nanosystems are among the most intensively investigated fields in nanotechnology. A relevant point to be considered in the design and development of nanovehicles intended for medical use is the formation of the “protein corona” around the nanoparticle,...
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doaj-32b191ec1d0443b0856a3a84bda8793d2020-11-24T23:17:03ZengHindawi LimitedJournal of Nanomaterials1687-41101687-41292017-01-01201710.1155/2017/65091846509184Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon ResonanceMauricio E. Di Ianni0Germán A. Islan1Cecilia Y. Chain2Guillermo R. Castro3Alan Talevi4María E. Vela5Laboratorio de Investigación y Desarrollo de Bioactivos (LIDeB), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, ArgentinaLaboratorio de Nanobiomateriales, Centro de Investigación y Desarrollo de Fermentaciones Industriales (CINDEFI), UNLP-CONICET, CCT La Plata, Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47 y 115, La Plata, ArgentinaInstituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), CONICET-UNLP, CC 16 Suc 4, La Plata, 1900 Buenos Aires, ArgentinaLaboratorio de Nanobiomateriales, Centro de Investigación y Desarrollo de Fermentaciones Industriales (CINDEFI), UNLP-CONICET, CCT La Plata, Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47 y 115, La Plata, ArgentinaLaboratorio de Investigación y Desarrollo de Bioactivos (LIDeB), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, ArgentinaInstituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), CONICET-UNLP, CC 16 Suc 4, La Plata, 1900 Buenos Aires, ArgentinaThe applications of pharmaceutical and medical nanosystems are among the most intensively investigated fields in nanotechnology. A relevant point to be considered in the design and development of nanovehicles intended for medical use is the formation of the “protein corona” around the nanoparticle, that is, a complex biomolecular layer formed when the nanovehicle is exposed to biological fluids. The chemical nature of the protein corona determines the biological identity of the nanoparticle and influences, among others, the recognition of the nanocarrier by the mononuclear phagocytic system and, thus, its clearance from the blood. Recent works suggest that Surface Plasmon Resonance (SPR), extensively employed for the analysis of biomolecular interactions, can shed light on the formation of the protein corona and its interaction with the surroundings. The synthesis and characterization of solid lipid nanoparticles (SLN) coated with polymers of different chemical nature (e.g., polyvinyl alcohol, chitosans) are reported. The proof-of-concept for the use of SPR technique in characterizing protein-nanoparticle interactions of surface-immobilized proteins (immunoglobulin G and bovine serum albumin, both involved in the formation of the corona) subjected to flowing SLN is demonstrated for non-chitosan-coated nanoparticles. All assayed nanosystems show more preference for IgG than for BSA, such preference being more pronounced in the case of polyvinyl-alcohol-coated SLN.http://dx.doi.org/10.1155/2017/6509184 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mauricio E. Di Ianni Germán A. Islan Cecilia Y. Chain Guillermo R. Castro Alan Talevi María E. Vela |
spellingShingle |
Mauricio E. Di Ianni Germán A. Islan Cecilia Y. Chain Guillermo R. Castro Alan Talevi María E. Vela Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon Resonance Journal of Nanomaterials |
author_facet |
Mauricio E. Di Ianni Germán A. Islan Cecilia Y. Chain Guillermo R. Castro Alan Talevi María E. Vela |
author_sort |
Mauricio E. Di Ianni |
title |
Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon Resonance |
title_short |
Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon Resonance |
title_full |
Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon Resonance |
title_fullStr |
Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon Resonance |
title_full_unstemmed |
Interaction of Solid Lipid Nanoparticles and Specific Proteins of the Corona Studied by Surface Plasmon Resonance |
title_sort |
interaction of solid lipid nanoparticles and specific proteins of the corona studied by surface plasmon resonance |
publisher |
Hindawi Limited |
series |
Journal of Nanomaterials |
issn |
1687-4110 1687-4129 |
publishDate |
2017-01-01 |
description |
The applications of pharmaceutical and medical nanosystems are among the most intensively investigated fields in nanotechnology. A relevant point to be considered in the design and development of nanovehicles intended for medical use is the formation of the “protein corona” around the nanoparticle, that is, a complex biomolecular layer formed when the nanovehicle is exposed to biological fluids. The chemical nature of the protein corona determines the biological identity of the nanoparticle and influences, among others, the recognition of the nanocarrier by the mononuclear phagocytic system and, thus, its clearance from the blood. Recent works suggest that Surface Plasmon Resonance (SPR), extensively employed for the analysis of biomolecular interactions, can shed light on the formation of the protein corona and its interaction with the surroundings. The synthesis and characterization of solid lipid nanoparticles (SLN) coated with polymers of different chemical nature (e.g., polyvinyl alcohol, chitosans) are reported. The proof-of-concept for the use of SPR technique in characterizing protein-nanoparticle interactions of surface-immobilized proteins (immunoglobulin G and bovine serum albumin, both involved in the formation of the corona) subjected to flowing SLN is demonstrated for non-chitosan-coated nanoparticles. All assayed nanosystems show more preference for IgG than for BSA, such preference being more pronounced in the case of polyvinyl-alcohol-coated SLN. |
url |
http://dx.doi.org/10.1155/2017/6509184 |
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