Abscisic Acid Is a Major Regulator of Grape Berry Ripening Onset: New Insights into ABA Signaling Network
Grapevine is a world-wide cultivated economically relevant crop. The process of berry ripening is non-climacteric and does not rely on the sole ethylene signal. Abscisic acid (ABA) is recognized as an important hormone of ripening inception and color development in ripening berries. In order to eluc...
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doaj-32c2d64a95c34ad4b329fe3ee14f3e422020-11-24T21:33:06ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-06-01810.3389/fpls.2017.01093264675Abscisic Acid Is a Major Regulator of Grape Berry Ripening Onset: New Insights into ABA Signaling NetworkStefania Pilati0Giorgia Bagagli1Paolo Sonego2Marco Moretto3Daniele Brazzale4Giulia Castorina5Laura Simoni6Chiara Tonelli7Graziano Guella8Graziano Guella9Kristof Engelen10Massimo Galbiati11Claudio Moser12Research and Innovation Centre, Fondazione Edmund MachSan Michele all′Adige, ItalyResearch and Innovation Centre, Fondazione Edmund MachSan Michele all′Adige, ItalyResearch and Innovation Centre, Fondazione Edmund MachSan Michele all′Adige, ItalyResearch and Innovation Centre, Fondazione Edmund MachSan Michele all′Adige, ItalyResearch and Innovation Centre, Fondazione Edmund MachSan Michele all′Adige, ItalyDipartimento di Bioscienze, Università degli Studi di MilanoMilan, ItalyDipartimento di Bioscienze, Università degli Studi di MilanoMilan, ItalyDipartimento di Bioscienze, Università degli Studi di MilanoMilan, ItalyDepartment of Physics, Bioorganic Chemistry Lab, University of TrentoTrento, ItalyIstituto di Biofisica, Consiglio Nazionale delle RicercheTrento, ItalyResearch and Innovation Centre, Fondazione Edmund MachSan Michele all′Adige, ItalyDipartimento di Bioscienze, Università degli Studi di MilanoMilan, ItalyResearch and Innovation Centre, Fondazione Edmund MachSan Michele all′Adige, ItalyGrapevine is a world-wide cultivated economically relevant crop. The process of berry ripening is non-climacteric and does not rely on the sole ethylene signal. Abscisic acid (ABA) is recognized as an important hormone of ripening inception and color development in ripening berries. In order to elucidate the effect of this signal at the molecular level, pre-véraison berries were treated ex vivo for 20 h with 0.2 mM ABA and berry skin transcriptional modulation was studied by RNA-seq after the treatment and 24 h later, in the absence of exogenous ABA. This study highlighted that a small amount of ABA triggered its own biosynthesis and had a transcriptome-wide effect (1893 modulated genes) characterized by the amplification of the transcriptional response over time. By comparing this dataset with the many studies on ripening collected within the grapevine transcriptomic compendium Vespucci, an extended overlap between ABA- and ripening modulated gene sets was observed (71% of the genes), underpinning the role of this hormone in the regulation of berry ripening. The signaling network of ABA, encompassing ABA metabolism, transport and signaling cascade, has been analyzed in detail and expanded based on knowledge from other species in order to provide an integrated molecular description of this pathway at berry ripening onset. Expression data analysis was combined with in silico promoter analysis to identify candidate target genes of ABA responsive element binding protein 2 (VvABF2), a key upstream transcription factor of the ABA signaling cascade which is up-regulated at véraison and also by ABA treatments. Two transcription factors, VvMYB143 and VvNAC17, and two genes involved in protein degradation, Armadillo-like and Xerico-like genes, were selected for in vivo validation by VvABF2-mediated promoter trans-activation in tobacco. VvNAC17 and Armadillo-like promoters were induced by ABA via VvABF2, while VvMYB143 responded to ABA in a VvABF2-independent manner. This knowledge of the ABA cascade in berry skin contributes not only to the understanding of berry ripening regulation but might be useful to other areas of viticultural interest, such as bud dormancy regulation and drought stress tolerance.http://journal.frontiersin.org/article/10.3389/fpls.2017.01093/fullAbscisic acid (ABA)grapevine (Vitis vinifera)berry ripeningRNA sequencingpromoter analysisAREB/ABF |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Stefania Pilati Giorgia Bagagli Paolo Sonego Marco Moretto Daniele Brazzale Giulia Castorina Laura Simoni Chiara Tonelli Graziano Guella Graziano Guella Kristof Engelen Massimo Galbiati Claudio Moser |
spellingShingle |
Stefania Pilati Giorgia Bagagli Paolo Sonego Marco Moretto Daniele Brazzale Giulia Castorina Laura Simoni Chiara Tonelli Graziano Guella Graziano Guella Kristof Engelen Massimo Galbiati Claudio Moser Abscisic Acid Is a Major Regulator of Grape Berry Ripening Onset: New Insights into ABA Signaling Network Frontiers in Plant Science Abscisic acid (ABA) grapevine (Vitis vinifera) berry ripening RNA sequencing promoter analysis AREB/ABF |
author_facet |
Stefania Pilati Giorgia Bagagli Paolo Sonego Marco Moretto Daniele Brazzale Giulia Castorina Laura Simoni Chiara Tonelli Graziano Guella Graziano Guella Kristof Engelen Massimo Galbiati Claudio Moser |
author_sort |
Stefania Pilati |
title |
Abscisic Acid Is a Major Regulator of Grape Berry Ripening Onset: New Insights into ABA Signaling Network |
title_short |
Abscisic Acid Is a Major Regulator of Grape Berry Ripening Onset: New Insights into ABA Signaling Network |
title_full |
Abscisic Acid Is a Major Regulator of Grape Berry Ripening Onset: New Insights into ABA Signaling Network |
title_fullStr |
Abscisic Acid Is a Major Regulator of Grape Berry Ripening Onset: New Insights into ABA Signaling Network |
title_full_unstemmed |
Abscisic Acid Is a Major Regulator of Grape Berry Ripening Onset: New Insights into ABA Signaling Network |
title_sort |
abscisic acid is a major regulator of grape berry ripening onset: new insights into aba signaling network |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2017-06-01 |
description |
Grapevine is a world-wide cultivated economically relevant crop. The process of berry ripening is non-climacteric and does not rely on the sole ethylene signal. Abscisic acid (ABA) is recognized as an important hormone of ripening inception and color development in ripening berries. In order to elucidate the effect of this signal at the molecular level, pre-véraison berries were treated ex vivo for 20 h with 0.2 mM ABA and berry skin transcriptional modulation was studied by RNA-seq after the treatment and 24 h later, in the absence of exogenous ABA. This study highlighted that a small amount of ABA triggered its own biosynthesis and had a transcriptome-wide effect (1893 modulated genes) characterized by the amplification of the transcriptional response over time. By comparing this dataset with the many studies on ripening collected within the grapevine transcriptomic compendium Vespucci, an extended overlap between ABA- and ripening modulated gene sets was observed (71% of the genes), underpinning the role of this hormone in the regulation of berry ripening. The signaling network of ABA, encompassing ABA metabolism, transport and signaling cascade, has been analyzed in detail and expanded based on knowledge from other species in order to provide an integrated molecular description of this pathway at berry ripening onset. Expression data analysis was combined with in silico promoter analysis to identify candidate target genes of ABA responsive element binding protein 2 (VvABF2), a key upstream transcription factor of the ABA signaling cascade which is up-regulated at véraison and also by ABA treatments. Two transcription factors, VvMYB143 and VvNAC17, and two genes involved in protein degradation, Armadillo-like and Xerico-like genes, were selected for in vivo validation by VvABF2-mediated promoter trans-activation in tobacco. VvNAC17 and Armadillo-like promoters were induced by ABA via VvABF2, while VvMYB143 responded to ABA in a VvABF2-independent manner. This knowledge of the ABA cascade in berry skin contributes not only to the understanding of berry ripening regulation but might be useful to other areas of viticultural interest, such as bud dormancy regulation and drought stress tolerance. |
topic |
Abscisic acid (ABA) grapevine (Vitis vinifera) berry ripening RNA sequencing promoter analysis AREB/ABF |
url |
http://journal.frontiersin.org/article/10.3389/fpls.2017.01093/full |
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