| Summary: | Today, the overall occurrence of re-emerging and rising illnesses has been a serious load on economies as well as public health. Here, we describe a simple, nontoxic and eco-friendly method for the synthesis of milk protein (MP)-stabilized silver nanoparticles (MP-s-AgNPs) using ultrahigh-temperature full cream milk. Highly stable AgNPs were prepared with a fair control over their size, without using any reducing or stabilizing agent, and their formation was attributed to the presence of the MP casein. Ag<sup>+</sup> ion reduction was possibly caused by the MPs. The synthesized MP-s-AgNPs were characterized in detail by ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, transmission electron microscopy and dynamic light scattering. MP-s-AgNPs showed inhibitory activity against both Gram-positive (<i>Bacillus subtilis</i> and <i>Staphylococcus aureus</i>) and Gram-negative microorganisms (<i>Salmonella typhi</i> and <i>Escherichia coli</i>). Moreover, MP-s-AgNPs were found to be more toxic to bacteria than to fungi (<i>Aspergillus fumigatus</i>, <i>Aspergillus ochraceus</i> and <i>Penicillium chrysogenum).</i>
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