Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to Haemonchus contortus infection in goats
Abstract Background Haemonchosis is a major economic problem in goat production in humid, tropical and subtropical regions. The disease is caused by an abomasal nematode, Haemonchus contortus, which is highly pathogenic in small ruminants. The aim of this study was to identifying single-nucleotide p...
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2019-03-01
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Series: | Journal of Animal Science and Biotechnology |
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Online Access: | http://link.springer.com/article/10.1186/s40104-019-0327-8 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mahmuda Bilkis Bintee Alam Abdullah Ibne Omar Md. Omar Faruque David Russell Notter Kathiravan Periasamy Md. Motahar Hussain Mondal Md. Jalal Uddin Sarder Md. Shamsuddin Jianhua Cao Xiaoyong Du Zhenyang Wu Shuhong Zhao |
spellingShingle |
Mahmuda Bilkis Bintee Alam Abdullah Ibne Omar Md. Omar Faruque David Russell Notter Kathiravan Periasamy Md. Motahar Hussain Mondal Md. Jalal Uddin Sarder Md. Shamsuddin Jianhua Cao Xiaoyong Du Zhenyang Wu Shuhong Zhao Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to Haemonchus contortus infection in goats Journal of Animal Science and Biotechnology Fecal egg count Goat Haemonchus contortus Parasite resistance SNP markers |
author_facet |
Mahmuda Bilkis Bintee Alam Abdullah Ibne Omar Md. Omar Faruque David Russell Notter Kathiravan Periasamy Md. Motahar Hussain Mondal Md. Jalal Uddin Sarder Md. Shamsuddin Jianhua Cao Xiaoyong Du Zhenyang Wu Shuhong Zhao |
author_sort |
Mahmuda Bilkis Bintee Alam |
title |
Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to Haemonchus contortus infection in goats |
title_short |
Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to Haemonchus contortus infection in goats |
title_full |
Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to Haemonchus contortus infection in goats |
title_fullStr |
Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to Haemonchus contortus infection in goats |
title_full_unstemmed |
Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to Haemonchus contortus infection in goats |
title_sort |
single nucleotide polymorphisms in candidate genes are significantly associated with resistance to haemonchus contortus infection in goats |
publisher |
BMC |
series |
Journal of Animal Science and Biotechnology |
issn |
2049-1891 |
publishDate |
2019-03-01 |
description |
Abstract Background Haemonchosis is a major economic problem in goat production in humid, tropical and subtropical regions. The disease is caused by an abomasal nematode, Haemonchus contortus, which is highly pathogenic in small ruminants. The aim of this study was to identifying single-nucleotide polymorphisms (SNP) that were associated with fecal egg counts (FEC) and could be used as markers to identify resistance to H. contortus in goats. Results Ten novel variants in the CIITA, ATP2A3, HSPA8, STAT5B, ESYT1, and SERPING1 genes were associated with FEC in goats with a nominal significance level of P < 0.05. Two missense mutation in the exon region of the caprine CIITA gene resulted in replacement of arginine with cysteine at position 9473550 (R9473550C) and aspartic acid with glutamic acid at position 9473870 (D9473870E). Chinese goat breeds had significantly higher FEC than Bangladeshi goat breeds within their respective genotypes. Polymorphism information content (PIC), effective allele number (Ne), and heterozygosity (He) were greatest for the STAT5B_197_A > G SNP locus in all goat breeds. Pairwise coefficients of linkage disequilibrium (D´, r 2) revealed complete LD (r 2 = 1) between significant SNP polymorphisms in CIITA and SERPING1 and strong LD (r 2 = 0.93 and 0.98) between polymorphisms in HSPA8 and ATP2A3, respectively. Correlation coefficient (r) between FEC and body weight (BW) was significantly positive (r = 0.56***, P < 0.001) but that between FEC and packed cell volume (PCV) was negatively significant (r = − 0.47**, P < 0.01) in the total population of goats. On the other hand, correlation coefficient (r) between BW and PCV was not significant in total population of goats. Association analysis revealed that haplotypes within ATP2A3, HSPA8, and SERPING1 were significantly associated with FEC. Quantitative real-time PCR revealed that the relative expression of mRNA was higher (P < 0.001) for resistant, compared to susceptible, groups of goats for all candidate genes except CIITA. Conclusions This study identified SNP markers that can potentially be used in marker-assisted selection programs to develop goat breeds that are resistant to H. contortus. |
topic |
Fecal egg count Goat Haemonchus contortus Parasite resistance SNP markers |
url |
http://link.springer.com/article/10.1186/s40104-019-0327-8 |
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doaj-331825becac440da87a982c4331c58b52020-11-25T02:59:32ZengBMCJournal of Animal Science and Biotechnology2049-18912019-03-0110111510.1186/s40104-019-0327-8Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to Haemonchus contortus infection in goatsMahmuda Bilkis Bintee Alam0Abdullah Ibne Omar1Md. Omar Faruque2David Russell Notter3Kathiravan Periasamy4Md. Motahar Hussain Mondal5Md. Jalal Uddin Sarder6Md. Shamsuddin7Jianhua Cao8Xiaoyong Du9Zhenyang Wu10Shuhong Zhao11Key Lab of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural UniversityKey Lab of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural UniversityDepartment of Animal Breeding and Genetics, Bangladesh Agricultural UniversityDepartment of Animal and Poultry Sciences, Virginia TechAnimal Production and Health Laboratory, Join FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy AgencyDepartment of Parasitology, Bangladesh Agricultural UniversityDepartment of Veterinary and Animal Science, University of RajshahiAnimal Production and Health Laboratory, Join FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy AgencyKey Lab of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural UniversityAgricultural Bioinformatics Key Laboratory of Hubei Province, College of Informatics, Huazhong Agricultural UniversityKey Lab of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural UniversityKey Lab of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural UniversityAbstract Background Haemonchosis is a major economic problem in goat production in humid, tropical and subtropical regions. The disease is caused by an abomasal nematode, Haemonchus contortus, which is highly pathogenic in small ruminants. The aim of this study was to identifying single-nucleotide polymorphisms (SNP) that were associated with fecal egg counts (FEC) and could be used as markers to identify resistance to H. contortus in goats. Results Ten novel variants in the CIITA, ATP2A3, HSPA8, STAT5B, ESYT1, and SERPING1 genes were associated with FEC in goats with a nominal significance level of P < 0.05. Two missense mutation in the exon region of the caprine CIITA gene resulted in replacement of arginine with cysteine at position 9473550 (R9473550C) and aspartic acid with glutamic acid at position 9473870 (D9473870E). Chinese goat breeds had significantly higher FEC than Bangladeshi goat breeds within their respective genotypes. Polymorphism information content (PIC), effective allele number (Ne), and heterozygosity (He) were greatest for the STAT5B_197_A > G SNP locus in all goat breeds. Pairwise coefficients of linkage disequilibrium (D´, r 2) revealed complete LD (r 2 = 1) between significant SNP polymorphisms in CIITA and SERPING1 and strong LD (r 2 = 0.93 and 0.98) between polymorphisms in HSPA8 and ATP2A3, respectively. Correlation coefficient (r) between FEC and body weight (BW) was significantly positive (r = 0.56***, P < 0.001) but that between FEC and packed cell volume (PCV) was negatively significant (r = − 0.47**, P < 0.01) in the total population of goats. On the other hand, correlation coefficient (r) between BW and PCV was not significant in total population of goats. Association analysis revealed that haplotypes within ATP2A3, HSPA8, and SERPING1 were significantly associated with FEC. Quantitative real-time PCR revealed that the relative expression of mRNA was higher (P < 0.001) for resistant, compared to susceptible, groups of goats for all candidate genes except CIITA. Conclusions This study identified SNP markers that can potentially be used in marker-assisted selection programs to develop goat breeds that are resistant to H. contortus.http://link.springer.com/article/10.1186/s40104-019-0327-8Fecal egg countGoatHaemonchus contortusParasite resistanceSNP markers |