In vitro Antioxidant Activities of Sodium Zinc and Sodium Iron Chlorophyllins from Pine Needles
Chlorophyll was extracted from pine needles, and then sodium zinc chlorophyllin (SZC) and sodium iron chlorophyllin (SIC) were synthesised by saponification, purification and substitution reaction, using sodium copper chlorophyllin (SCC) as a control. Their crystalline structures were verified by at...
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doaj-331f7de36b254418913849ea142ec1742020-11-25T02:18:19ZengUniversity of ZagrebFood Technology and Biotechnology1330-98621334-26062014-01-01524505510In vitro Antioxidant Activities of Sodium Zinc and Sodium Iron Chlorophyllins from Pine NeedlesRuzhen Zhan0Jian Wu1Jie Ouyang2Department of Food Science and Engineering, College of Biological Sciences and Technology, Beij ing Forestry University, Beij ing 100083, PR ChinaDepartment of Food Science and Engineering, College of Biological Sciences and Technology, Beij ing Forestry University, Beij ing 100083, PR ChinaDepartment of Food Science and Engineering, College of Biological Sciences and Technology, Beij ing Forestry University, Beij ing 100083, PR ChinaChlorophyll was extracted from pine needles, and then sodium zinc chlorophyllin (SZC) and sodium iron chlorophyllin (SIC) were synthesised by saponification, purification and substitution reaction, using sodium copper chlorophyllin (SCC) as a control. Their crystalline structures were verified by atomic absorbance spectroscopy, UV-VIS spectroscopy and HPLC. Moreover, their antioxidant activities were evaluated and compared with that of ascorbic acid through four biochemical assays: DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power, inhibition of β-carotene bleaching and O2– scavenging activity. SZC had better antioxidant properties at a lower dosage than SIC and SCC in all assays. In the β-carotene bleaching assay, EC50 of SZC, SIC and SCC was 0.04, 0.38 and 0.90 mg/mL, respectively, much lower than that of ascorbic acid (4.0 mg/mL). SZC showed a better result (p<0.05) than ascorbic acid in the O2 – scavenging activity assay. The results obtained from reducing power determination were also excellent: the absorbance values were all about 1.0 at 0.5 mg/mL, about half of that of ascorbic acid. In the investigation of DPPH radical scavenging activity, all chlorophyllins had lower activities than ascorbic acid. These results demonstrated the potential bioactivities of chlorophyll derivatives and supported their possible role in human health protection and disease prevention.http://hrcak.srce.hr/file/193465sodium zinc chlorophyllinsodium iron chlorophyllinsodium copper chlorophyllinantioxidant activitypine needles |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ruzhen Zhan Jian Wu Jie Ouyang |
spellingShingle |
Ruzhen Zhan Jian Wu Jie Ouyang In vitro Antioxidant Activities of Sodium Zinc and Sodium Iron Chlorophyllins from Pine Needles Food Technology and Biotechnology sodium zinc chlorophyllin sodium iron chlorophyllin sodium copper chlorophyllin antioxidant activity pine needles |
author_facet |
Ruzhen Zhan Jian Wu Jie Ouyang |
author_sort |
Ruzhen Zhan |
title |
In vitro Antioxidant Activities of Sodium Zinc and Sodium Iron Chlorophyllins from Pine Needles |
title_short |
In vitro Antioxidant Activities of Sodium Zinc and Sodium Iron Chlorophyllins from Pine Needles |
title_full |
In vitro Antioxidant Activities of Sodium Zinc and Sodium Iron Chlorophyllins from Pine Needles |
title_fullStr |
In vitro Antioxidant Activities of Sodium Zinc and Sodium Iron Chlorophyllins from Pine Needles |
title_full_unstemmed |
In vitro Antioxidant Activities of Sodium Zinc and Sodium Iron Chlorophyllins from Pine Needles |
title_sort |
in vitro antioxidant activities of sodium zinc and sodium iron chlorophyllins from pine needles |
publisher |
University of Zagreb |
series |
Food Technology and Biotechnology |
issn |
1330-9862 1334-2606 |
publishDate |
2014-01-01 |
description |
Chlorophyll was extracted from pine needles, and then sodium zinc chlorophyllin (SZC) and sodium iron chlorophyllin (SIC) were synthesised by saponification, purification and substitution reaction, using sodium copper chlorophyllin (SCC) as a control. Their crystalline structures were verified by atomic absorbance spectroscopy, UV-VIS spectroscopy and HPLC. Moreover, their antioxidant activities were evaluated and compared with that of ascorbic acid through four biochemical assays: DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power, inhibition of β-carotene bleaching and O2– scavenging activity. SZC had better antioxidant properties at a lower dosage than SIC and SCC in all assays. In the β-carotene bleaching assay, EC50 of SZC, SIC and SCC was 0.04, 0.38 and 0.90 mg/mL, respectively, much lower than that of ascorbic acid (4.0 mg/mL). SZC showed a better result (p<0.05) than ascorbic acid in the O2 – scavenging activity assay. The results obtained from reducing power determination were also excellent: the absorbance values were all about 1.0 at 0.5 mg/mL, about half of that of ascorbic acid. In the investigation of DPPH radical scavenging activity, all chlorophyllins had lower activities than ascorbic acid. These results demonstrated the potential bioactivities of chlorophyll derivatives and supported their possible role in human health protection and disease prevention. |
topic |
sodium zinc chlorophyllin sodium iron chlorophyllin sodium copper chlorophyllin antioxidant activity pine needles |
url |
http://hrcak.srce.hr/file/193465 |
work_keys_str_mv |
AT ruzhenzhan invitroantioxidantactivitiesofsodiumzincandsodiumironchlorophyllinsfrompineneedles AT jianwu invitroantioxidantactivitiesofsodiumzincandsodiumironchlorophyllinsfrompineneedles AT jieouyang invitroantioxidantactivitiesofsodiumzincandsodiumironchlorophyllinsfrompineneedles |
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1724882987778048000 |