Efficacy of (+)-Lariciresinol to Control Bacterial Growth of Staphylococcus aureus and Escherichia coli O157:H7

• Main Authors: Vivek K. Bajpai, Shruti Shukla, Woon K. Paek, Jeongheui Lim, Pradeep Kumar, Pankaj Kumar, MinKyun Na
• Format: Article
• Language: English
• Published: Frontiers Media S.A. 2017-05-01
• Series: Frontiers in Microbiology
• Subjects: antimicrobial effect; Rubia philippinensis; (+)-lariciresinol; foodborne pathogens; scanning electron microscopy
• Online Access: http://journal.frontiersin.org/article/10.3389/fmicb.2017.00804/full

This study was undertaken to assess the antibacterial potential of a polyphenolic compound (+)-lariciresinol isolated from Rubia philippinensis against selected foodborne pathogens Staphylococcus aureus KCTC1621 and Escherichia coli O157:H7. (+)-Lariciresinol at the tested concentrations (250 μg/disk) evoked a significant antibacterial effect as a diameter of inhibition zones (12.1–14.9 mm) with minimum inhibitory concentration (MIC), and minimum bactericidal concentration values of 125–250 and 125–250 μg/mL, respectively. Furthermore, (+)-lariciresinol at MIC showed reduction in bacterial cell viabilities, efflux of potassium (K+) ions and release of 260 nm materials against E. coli O157:H7 and S. aureus KCTC1621. Moreover, deteriorated cell wall morphology of E. coli O157:H7 and S. aureus KCTC1621 cells treated with (+)-lariciresinol at MIC further confirmed its inhibitory effect against the tested pathogens, suggesting it to be an alternative means of antimicrobials.