Electrochemiluminesence Epidemiologic Detection of Toxoplasma gondii Infection in Pregnant Women With Direct and Indirect Diagnostic Techniques (ELISA Avidity Plus Biochemical Assay)

Background and aims: Toxoplasmosis is a very common disease in the world. Two types of acquired toxoplasmosis have been detected. In the chronic toxoplasmosis, the abnormality of tissue function is little but, in acute toxoplasmosis, function of RES system becomes interrupted. The assessment of toxo...

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Main Authors: Ehsan Shariat Bahadory, Ali dalir Ghaffari, Somayyeh Namrood, Seyyedeh Somayyeh Mosavipour, Javid Sadraei
Format: Article
Language:English
Published: Shahrekord University of Medical Sciences 2018-01-01
Series:International Journal of Epidemiologic Research
Subjects:
Online Access:http://ijer.skums.ac.ir/article_30863_357f6c5285f307560e6ef054eacb1310.pdf
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spelling doaj-33275faea3dc49cfb9ade30c226a89d32020-11-25T01:34:51ZengShahrekord University of Medical SciencesInternational Journal of Epidemiologic Research2383-43662018-01-0151242910.15171/ijer.2018.06ijer-16Electrochemiluminesence Epidemiologic Detection of Toxoplasma gondii Infection in Pregnant Women With Direct and Indirect Diagnostic Techniques (ELISA Avidity Plus Biochemical Assay)Ehsan Shariat Bahadory0Ali dalir Ghaffari1Somayyeh Namrood2Seyyedeh Somayyeh Mosavipour3Javid Sadraei4Department of Medical Parasitology, Faculty of Medical Sciences, Tarbiat Modaress University, Tehran, IranDepartment of Medical Parasitology, Faculty of Medical Sciences, Tarbiat Modaress University, Tehran, IranAssistant professor, Department of Environmental sciences, Faculty of Fisheries and Environmental Sciences, University of Agricultural Sciences & Natural Resources, Gorgan, IranDepartment of Nursing, Faculty of Medical Sciences, Tehran Medical Sciences University, Tehran, IranDepartment of Medical Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranBackground and aims: Toxoplasmosis is a very common disease in the world. Two types of acquired toxoplasmosis have been detected. In the chronic toxoplasmosis, the abnormality of tissue function is little but, in acute toxoplasmosis, function of RES system becomes interrupted. The assessment of toxoplasma antibody with tissue enzymes in this stage is very important. Furthermore, serum ferritin in some conditions became high in acute phase of infectious disease. Methods: This study was based on comparative abundance study. Materials consisted of 980 serum and amniotic fluid samples collected from human blood with high level of IgG antibody against Toxoplasma gondii in Rajaie center, Tehran, Iran. The standard test was ELISA assay to detect these antibodies and the main test was measurement of liver transaminases (SGOT, SGPT) bilirubin and ferritin to evaluate acute toxoplasmosis. Data analysis was done by SPSS version 18.0. This study was done during March to June, 2017. Results: Results showed that in some patients with high level of IgG AVIDITY antibody against T. gondii the level of liver transaminases, serum bilirubin and ferritin became increased. For example in 120 patients with acute toxoplasmosis the mean serum levels of SGOT were 108 IU/L and in 80 patients the mean serum bilirubin were about 5 mg/dL. Conclusion: In acute congenital toxoplasmosis, the evaluation of IgG AVIDITY was first step and then the measurement of biochemical factors such as serum transaminases, serum Bilirubin and serum Ferritin were important.http://ijer.skums.ac.ir/article_30863_357f6c5285f307560e6ef054eacb1310.pdfToxoplasmosisBilirubinElectrochemiluminesenceFerritinIgG AvidityLiver function
collection DOAJ
language English
format Article
sources DOAJ
author Ehsan Shariat Bahadory
Ali dalir Ghaffari
Somayyeh Namrood
Seyyedeh Somayyeh Mosavipour
Javid Sadraei
spellingShingle Ehsan Shariat Bahadory
Ali dalir Ghaffari
Somayyeh Namrood
Seyyedeh Somayyeh Mosavipour
Javid Sadraei
Electrochemiluminesence Epidemiologic Detection of Toxoplasma gondii Infection in Pregnant Women With Direct and Indirect Diagnostic Techniques (ELISA Avidity Plus Biochemical Assay)
International Journal of Epidemiologic Research
Toxoplasmosis
Bilirubin
Electrochemiluminesence
Ferritin
IgG Avidity
Liver function
author_facet Ehsan Shariat Bahadory
Ali dalir Ghaffari
Somayyeh Namrood
Seyyedeh Somayyeh Mosavipour
Javid Sadraei
author_sort Ehsan Shariat Bahadory
title Electrochemiluminesence Epidemiologic Detection of Toxoplasma gondii Infection in Pregnant Women With Direct and Indirect Diagnostic Techniques (ELISA Avidity Plus Biochemical Assay)
title_short Electrochemiluminesence Epidemiologic Detection of Toxoplasma gondii Infection in Pregnant Women With Direct and Indirect Diagnostic Techniques (ELISA Avidity Plus Biochemical Assay)
title_full Electrochemiluminesence Epidemiologic Detection of Toxoplasma gondii Infection in Pregnant Women With Direct and Indirect Diagnostic Techniques (ELISA Avidity Plus Biochemical Assay)
title_fullStr Electrochemiluminesence Epidemiologic Detection of Toxoplasma gondii Infection in Pregnant Women With Direct and Indirect Diagnostic Techniques (ELISA Avidity Plus Biochemical Assay)
title_full_unstemmed Electrochemiluminesence Epidemiologic Detection of Toxoplasma gondii Infection in Pregnant Women With Direct and Indirect Diagnostic Techniques (ELISA Avidity Plus Biochemical Assay)
title_sort electrochemiluminesence epidemiologic detection of toxoplasma gondii infection in pregnant women with direct and indirect diagnostic techniques (elisa avidity plus biochemical assay)
publisher Shahrekord University of Medical Sciences
series International Journal of Epidemiologic Research
issn 2383-4366
publishDate 2018-01-01
description Background and aims: Toxoplasmosis is a very common disease in the world. Two types of acquired toxoplasmosis have been detected. In the chronic toxoplasmosis, the abnormality of tissue function is little but, in acute toxoplasmosis, function of RES system becomes interrupted. The assessment of toxoplasma antibody with tissue enzymes in this stage is very important. Furthermore, serum ferritin in some conditions became high in acute phase of infectious disease. Methods: This study was based on comparative abundance study. Materials consisted of 980 serum and amniotic fluid samples collected from human blood with high level of IgG antibody against Toxoplasma gondii in Rajaie center, Tehran, Iran. The standard test was ELISA assay to detect these antibodies and the main test was measurement of liver transaminases (SGOT, SGPT) bilirubin and ferritin to evaluate acute toxoplasmosis. Data analysis was done by SPSS version 18.0. This study was done during March to June, 2017. Results: Results showed that in some patients with high level of IgG AVIDITY antibody against T. gondii the level of liver transaminases, serum bilirubin and ferritin became increased. For example in 120 patients with acute toxoplasmosis the mean serum levels of SGOT were 108 IU/L and in 80 patients the mean serum bilirubin were about 5 mg/dL. Conclusion: In acute congenital toxoplasmosis, the evaluation of IgG AVIDITY was first step and then the measurement of biochemical factors such as serum transaminases, serum Bilirubin and serum Ferritin were important.
topic Toxoplasmosis
Bilirubin
Electrochemiluminesence
Ferritin
IgG Avidity
Liver function
url http://ijer.skums.ac.ir/article_30863_357f6c5285f307560e6ef054eacb1310.pdf
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