Study of Methanol Extracts from Different Parts of Peganum harmala L. Using 1H-NMR Plant Metabolomics
A nuclear magnetic resonance- (NMR-) based metabolomics method was used to identify differential metabolites of methanol extracts obtained from six parts of Peganum harmala L. (P. harmala), namely, the root, stem, leaf, flower, testa, and seed. Two multivariate statistical analysis methods, principa...
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Series: | Journal of Analytical Methods in Chemistry |
Online Access: | http://dx.doi.org/10.1155/2018/6532789 |
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doaj-332b7110d5a74d41bde5d01660d1953f2020-11-25T03:27:03ZengHindawi LimitedJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/65327896532789Study of Methanol Extracts from Different Parts of Peganum harmala L. Using 1H-NMR Plant MetabolomicsYinping Li0Qing He1Shushan Du2Shanshan Guo3Zhufeng Geng4Zhiwei Deng5College of Chemistry, Beijing Normal University, Beijing 100875, ChinaSchool of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, ChinaBeijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Faculty of Geographical Science, Beijing Normal University, Beijing, ChinaBeijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Faculty of Geographical Science, Beijing Normal University, Beijing, ChinaBeijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Faculty of Geographical Science, Beijing Normal University, Beijing, ChinaAnalytic and Testing Center, Beijing Normal University, Beijing 100875, ChinaA nuclear magnetic resonance- (NMR-) based metabolomics method was used to identify differential metabolites of methanol extracts obtained from six parts of Peganum harmala L. (P. harmala), namely, the root, stem, leaf, flower, testa, and seed. Two multivariate statistical analysis methods, principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA), were combined to clearly distinguish among the P. harmala samples from the six different parts. Eleven differential components were screened by the PLS-DA loading plot, and the relative contents were calculated by univariate analysis of variance. Chemometric results showed significant differences in the metabolites of the different parts of P. harmala. The seeds contained large amounts of harmaline, harmine, and vasicine compared to other organs. The acetic acid, proline, lysine, and sucrose contents of the roots were significantly higher than those of the other parts. In the testa, the vasicine, asparagine, choline, and 4-hydroxyisoleucine contents were clearly dominant. The obtained data revealed the distribution characteristics of the metabolomes of the different P. harmala parts and provided fundamental knowledge for the rational development of its medicinal parts.http://dx.doi.org/10.1155/2018/6532789 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yinping Li Qing He Shushan Du Shanshan Guo Zhufeng Geng Zhiwei Deng |
spellingShingle |
Yinping Li Qing He Shushan Du Shanshan Guo Zhufeng Geng Zhiwei Deng Study of Methanol Extracts from Different Parts of Peganum harmala L. Using 1H-NMR Plant Metabolomics Journal of Analytical Methods in Chemistry |
author_facet |
Yinping Li Qing He Shushan Du Shanshan Guo Zhufeng Geng Zhiwei Deng |
author_sort |
Yinping Li |
title |
Study of Methanol Extracts from Different Parts of Peganum harmala L. Using 1H-NMR Plant Metabolomics |
title_short |
Study of Methanol Extracts from Different Parts of Peganum harmala L. Using 1H-NMR Plant Metabolomics |
title_full |
Study of Methanol Extracts from Different Parts of Peganum harmala L. Using 1H-NMR Plant Metabolomics |
title_fullStr |
Study of Methanol Extracts from Different Parts of Peganum harmala L. Using 1H-NMR Plant Metabolomics |
title_full_unstemmed |
Study of Methanol Extracts from Different Parts of Peganum harmala L. Using 1H-NMR Plant Metabolomics |
title_sort |
study of methanol extracts from different parts of peganum harmala l. using 1h-nmr plant metabolomics |
publisher |
Hindawi Limited |
series |
Journal of Analytical Methods in Chemistry |
issn |
2090-8865 2090-8873 |
publishDate |
2018-01-01 |
description |
A nuclear magnetic resonance- (NMR-) based metabolomics method was used to identify differential metabolites of methanol extracts obtained from six parts of Peganum harmala L. (P. harmala), namely, the root, stem, leaf, flower, testa, and seed. Two multivariate statistical analysis methods, principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA), were combined to clearly distinguish among the P. harmala samples from the six different parts. Eleven differential components were screened by the PLS-DA loading plot, and the relative contents were calculated by univariate analysis of variance. Chemometric results showed significant differences in the metabolites of the different parts of P. harmala. The seeds contained large amounts of harmaline, harmine, and vasicine compared to other organs. The acetic acid, proline, lysine, and sucrose contents of the roots were significantly higher than those of the other parts. In the testa, the vasicine, asparagine, choline, and 4-hydroxyisoleucine contents were clearly dominant. The obtained data revealed the distribution characteristics of the metabolomes of the different P. harmala parts and provided fundamental knowledge for the rational development of its medicinal parts. |
url |
http://dx.doi.org/10.1155/2018/6532789 |
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