In vitro larvicidal effects of ethanolic extract of Curcuma longa Linn. on Haemonchus larval stage
Aim: Gastrointestinal helminthosis is a global problem in small ruminant production. Most parasites have developed resistance to commonly available anthelminthic compounds, and there is currently an increasing need for new compounds with more efficacies. This study evaluated the in vitro effects of...
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doaj-3356d89ce1f440bb84bf6ee972144dd82021-08-02T14:25:13ZengVeterinary WorldVeterinary World0972-89882231-09162016-04-019441742010.14202/vetworld.2016.417-420In vitro larvicidal effects of ethanolic extract of Curcuma longa Linn. on Haemonchus larval stageNorisal Binti Nasai0Yusuf Abba1Faez Firdaus Jesse Abdullah2Murugaiyah Marimuthu3Abdulnasir Tijjani4Muhammad Abubakar Sadiq5Konto Mohammed6Eric Lim Teik Chung7Mohammed Ariff Bin Omar8Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; usuba5050@live.comDepartment of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; yabbavet@gmail.comDepartment of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Research Centre for Ruminant Disease, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; jesseariasamy@gmail.comDepartment of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; researchofficer2002@yahoo.comDepartment of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; nasirvet69@gmail.comDepartment of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; masadiqvet@gmail.comDepartment of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; kontomohammed@gmail.comDepartment of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; ericlim88@gmail.comDepartment of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Research Centre for Ruminant Disease, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; mo_ariff@upm.edu.myAim: Gastrointestinal helminthosis is a global problem in small ruminant production. Most parasites have developed resistance to commonly available anthelminthic compounds, and there is currently an increasing need for new compounds with more efficacies. This study evaluated the in vitro effects of ethanolic extract of Curcuma longa (EECL) as a biological nematicide against third stage Haemonchus larvae (L3) isolated from sheep. Materials and Methods: Haemonchus L3 were cultured and harvested from the feces of naturally infected sheep. EECL was prepared and three concentrations; 50, 100, and 200 mg/mL were tested for their efficacies on Haemonchus L3. Levamisole at concentration 1.5 and 3 mg/mL were used as positive controls. Results: EECL showed anthelmintic activity in a dose-dependent manner with 78% worm mortality within 24 h of exposure at the highest dose rate of 200 mg/mL. There was a 100% worm mortality rate after 2 h of levamisole (3 mg/mL) admisntration. However, there was a comparable larvicidal effect between when levamisole (1.5 mg/mL) and EECL (200 mg) were administered. Conclusion: The study shows that EECL does exhibit good anthelmintic properties at 200 mg/mL which is comparable with levamisole at 1.5 mg/mL.http://www.veterinaryworld.org/Vol.9/April-2016/14.pdfCurcuma longaethanolic extractHaemonchuslarvae 3levamisolestrongyle |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Norisal Binti Nasai Yusuf Abba Faez Firdaus Jesse Abdullah Murugaiyah Marimuthu Abdulnasir Tijjani Muhammad Abubakar Sadiq Konto Mohammed Eric Lim Teik Chung Mohammed Ariff Bin Omar |
spellingShingle |
Norisal Binti Nasai Yusuf Abba Faez Firdaus Jesse Abdullah Murugaiyah Marimuthu Abdulnasir Tijjani Muhammad Abubakar Sadiq Konto Mohammed Eric Lim Teik Chung Mohammed Ariff Bin Omar In vitro larvicidal effects of ethanolic extract of Curcuma longa Linn. on Haemonchus larval stage Veterinary World Curcuma longa ethanolic extract Haemonchus larvae 3 levamisole strongyle |
author_facet |
Norisal Binti Nasai Yusuf Abba Faez Firdaus Jesse Abdullah Murugaiyah Marimuthu Abdulnasir Tijjani Muhammad Abubakar Sadiq Konto Mohammed Eric Lim Teik Chung Mohammed Ariff Bin Omar |
author_sort |
Norisal Binti Nasai |
title |
In vitro larvicidal effects of ethanolic extract of Curcuma longa Linn. on Haemonchus larval stage |
title_short |
In vitro larvicidal effects of ethanolic extract of Curcuma longa Linn. on Haemonchus larval stage |
title_full |
In vitro larvicidal effects of ethanolic extract of Curcuma longa Linn. on Haemonchus larval stage |
title_fullStr |
In vitro larvicidal effects of ethanolic extract of Curcuma longa Linn. on Haemonchus larval stage |
title_full_unstemmed |
In vitro larvicidal effects of ethanolic extract of Curcuma longa Linn. on Haemonchus larval stage |
title_sort |
in vitro larvicidal effects of ethanolic extract of curcuma longa linn. on haemonchus larval stage |
publisher |
Veterinary World |
series |
Veterinary World |
issn |
0972-8988 2231-0916 |
publishDate |
2016-04-01 |
description |
Aim: Gastrointestinal helminthosis is a global problem in small ruminant production. Most parasites have developed resistance to commonly available anthelminthic compounds, and there is currently an increasing need for new compounds with more efficacies. This study evaluated the in vitro effects of ethanolic extract of Curcuma longa (EECL) as a biological nematicide against third stage Haemonchus larvae (L3) isolated from sheep.
Materials and Methods: Haemonchus L3 were cultured and harvested from the feces of naturally infected sheep. EECL was prepared and three concentrations; 50, 100, and 200 mg/mL were tested for their efficacies on Haemonchus L3. Levamisole at concentration 1.5 and 3 mg/mL were used as positive controls.
Results: EECL showed anthelmintic activity in a dose-dependent manner with 78% worm mortality within 24 h of exposure at the highest dose rate of 200 mg/mL. There was a 100% worm mortality rate after 2 h of levamisole (3 mg/mL) admisntration. However, there was a comparable larvicidal effect between when levamisole (1.5 mg/mL) and EECL (200 mg) were administered.
Conclusion: The study shows that EECL does exhibit good anthelmintic properties at 200 mg/mL which is comparable with levamisole at 1.5 mg/mL. |
topic |
Curcuma longa ethanolic extract Haemonchus larvae 3 levamisole strongyle |
url |
http://www.veterinaryworld.org/Vol.9/April-2016/14.pdf |
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