Identification of A Novel Picorna-Like Virus, Burpengary Virus, that is Negatively Associated with Chlamydial Disease in the Koala

Koalas (Phascolarctos cinereus) are native Australian marsupials whose populations are in decline from a range of threats. Infectious diseases caused by the bacterium Chlamydia pecorum and other pathogens are of particular concern. We analysed 26 poly-A selected RNA-sequencing libraries from a data...

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Main Authors:	Erin Harvey, Danielle Madden, Adam Polkinghorne, Edward C. Holmes
Format:	Article
Language:	English
Published:	MDPI AG 2019-03-01
Series:	Viruses
Subjects:	virus discovery Chlamydia pecorum koala picornavirus phylogeny
Online Access:	http://www.mdpi.com/1999-4915/11/3/211

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spelling	doaj-33603c1dd64545edb2421989690a76912020-11-24T21:20:53ZengMDPI AGViruses1999-49152019-03-0111321110.3390/v11030211v11030211Identification of A Novel Picorna-Like Virus, Burpengary Virus, that is Negatively Associated with Chlamydial Disease in the KoalaErin Harvey0Danielle Madden1Adam Polkinghorne2Edward C. Holmes3Marie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Life and Environmental Sciences and Sydney Medical School, The University of Sydney, Sydney, NSW 2006, AustraliaAnimal Research Centre, University of the Sunshine Coast, Sippy Downs, QLD 4556, AustraliaAnimal Research Centre, University of the Sunshine Coast, Sippy Downs, QLD 4556, AustraliaMarie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Life and Environmental Sciences and Sydney Medical School, The University of Sydney, Sydney, NSW 2006, AustraliaKoalas (Phascolarctos cinereus) are native Australian marsupials whose populations are in decline from a range of threats. Infectious diseases caused by the bacterium Chlamydia pecorum and other pathogens are of particular concern. We analysed 26 poly-A selected RNA-sequencing libraries from a data set designed to study the immune response of koalas to ocular chlamydial infection. Using virus discovery techniques, we identified the coding-complete genome sequence of a novel picorna-like virus, denoted Burpengary virus, that was most common in south-east Queensland. Notably, abundance measurements of the virus across all 26 libraries revealed an inverse relationship between abundance and ocular disease in koalas, suggesting that the co-infection of Burpengary virus and Chlamydia pecorum is inhibited.http://www.mdpi.com/1999-4915/11/3/211virus discoveryChlamydia pecorumkoalapicornavirusphylogeny
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Erin Harvey Danielle Madden Adam Polkinghorne Edward C. Holmes
spellingShingle	Erin Harvey Danielle Madden Adam Polkinghorne Edward C. Holmes Identification of A Novel Picorna-Like Virus, Burpengary Virus, that is Negatively Associated with Chlamydial Disease in the Koala Viruses virus discovery Chlamydia pecorum koala picornavirus phylogeny
author_facet	Erin Harvey Danielle Madden Adam Polkinghorne Edward C. Holmes
author_sort	Erin Harvey
title	Identification of A Novel Picorna-Like Virus, Burpengary Virus, that is Negatively Associated with Chlamydial Disease in the Koala
title_short	Identification of A Novel Picorna-Like Virus, Burpengary Virus, that is Negatively Associated with Chlamydial Disease in the Koala
title_full	Identification of A Novel Picorna-Like Virus, Burpengary Virus, that is Negatively Associated with Chlamydial Disease in the Koala
title_fullStr	Identification of A Novel Picorna-Like Virus, Burpengary Virus, that is Negatively Associated with Chlamydial Disease in the Koala
title_full_unstemmed	Identification of A Novel Picorna-Like Virus, Burpengary Virus, that is Negatively Associated with Chlamydial Disease in the Koala
title_sort	identification of a novel picorna-like virus, burpengary virus, that is negatively associated with chlamydial disease in the koala
publisher	MDPI AG
series	Viruses
issn	1999-4915
publishDate	2019-03-01
description	Koalas (Phascolarctos cinereus) are native Australian marsupials whose populations are in decline from a range of threats. Infectious diseases caused by the bacterium Chlamydia pecorum and other pathogens are of particular concern. We analysed 26 poly-A selected RNA-sequencing libraries from a data set designed to study the immune response of koalas to ocular chlamydial infection. Using virus discovery techniques, we identified the coding-complete genome sequence of a novel picorna-like virus, denoted Burpengary virus, that was most common in south-east Queensland. Notably, abundance measurements of the virus across all 26 libraries revealed an inverse relationship between abundance and ocular disease in koalas, suggesting that the co-infection of Burpengary virus and Chlamydia pecorum is inhibited.
topic	virus discovery Chlamydia pecorum koala picornavirus phylogeny
url	http://www.mdpi.com/1999-4915/11/3/211
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Identification of A Novel Picorna-Like Virus, Burpengary Virus, that is Negatively Associated with Chlamydial Disease in the Koala

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