Ratiometric Alcohol Sensor based on a Polymeric Nile Blue
We present a sterilizable ratiometric fluorescent ethanol sensor with sensitivity over a wide range (0-100%) of ethanol concentration v/v. The sensor is composed of a near infra red fluorescent solvatochromic dye, nile blue methacrylamide polymerized into a polyethylene (glycol) dimethacrylate matri...
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Academic Publishing House
2008-04-01
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Online Access: | http://www.clbme.bas.bg/bioautomation/2008/vol_9.1/files/9_2.3.pdf |
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doaj-33648587740d417a8d448294f07977fe2020-11-25T03:19:36ZengAcademic Publishing HouseBioautomation1313-261X1312-451X2008-04-01913139Ratiometric Alcohol Sensor based on a Polymeric Nile BlueSherif IbrahimNirmala ChandrasekharanYordan KostovGovind RaoWe present a sterilizable ratiometric fluorescent ethanol sensor with sensitivity over a wide range (0-100%) of ethanol concentration v/v. The sensor is composed of a near infra red fluorescent solvatochromic dye, nile blue methacrylamide polymerized into a polyethylene (glycol) dimethacrylate matrix. The dye can typically exhibit two or more wavelength dependent shifts in the fluorescence intensities based on its different micropolar environments. Two different concentrations of the nile blue methacrylamide dye were prepared and polymerized into homogenous films. The fluorescence properties of the two different films were investigated with a view to determining their ethanol sensing capabilities. The sensor was immersed in a water-ethanol solvent mixture. Excitation of the dye was performed at 470 nm. The range of emission wavelengths was 480-800 nm. The ratio of the fluorescence intensities at 620 nm and 554 nm was obtained for ethanol concentrations varying from 0-100% and the calibration curve of the ratiometric fluorescence intensities over the entire concentration range of ethanol was plotted. A ratiometric intensity change of over 33% has been obtained for pure ethanol compared to that obtained for pure water. The sensor response was rapid (≤10 minutes). The sterilizable ethanol sensor exhibits good potential for on-line monitoring of the ethanol generated in an LB fermentation chamber. http://www.clbme.bas.bg/bioautomation/2008/vol_9.1/files/9_2.3.pdf Alcohol sensingOptical measurementsFluorescenceRatiometric sensor |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sherif Ibrahim Nirmala Chandrasekharan Yordan Kostov Govind Rao |
spellingShingle |
Sherif Ibrahim Nirmala Chandrasekharan Yordan Kostov Govind Rao Ratiometric Alcohol Sensor based on a Polymeric Nile Blue Bioautomation Alcohol sensing Optical measurements Fluorescence Ratiometric sensor |
author_facet |
Sherif Ibrahim Nirmala Chandrasekharan Yordan Kostov Govind Rao |
author_sort |
Sherif Ibrahim |
title |
Ratiometric Alcohol Sensor based on a Polymeric Nile Blue |
title_short |
Ratiometric Alcohol Sensor based on a Polymeric Nile Blue |
title_full |
Ratiometric Alcohol Sensor based on a Polymeric Nile Blue |
title_fullStr |
Ratiometric Alcohol Sensor based on a Polymeric Nile Blue |
title_full_unstemmed |
Ratiometric Alcohol Sensor based on a Polymeric Nile Blue |
title_sort |
ratiometric alcohol sensor based on a polymeric nile blue |
publisher |
Academic Publishing House |
series |
Bioautomation |
issn |
1313-261X 1312-451X |
publishDate |
2008-04-01 |
description |
We present a sterilizable ratiometric fluorescent ethanol sensor with sensitivity over a wide range (0-100%) of ethanol concentration v/v. The sensor is composed of a near infra red fluorescent solvatochromic dye, nile blue methacrylamide polymerized into a polyethylene (glycol) dimethacrylate matrix. The dye can typically exhibit two or more wavelength dependent shifts in the fluorescence intensities based on its different micropolar environments. Two different concentrations of the nile blue methacrylamide dye were prepared and polymerized into homogenous films. The fluorescence properties of the two different films were investigated with a view to determining their ethanol sensing capabilities. The sensor was immersed in a water-ethanol solvent mixture. Excitation of the dye was performed at 470 nm. The range of emission wavelengths was 480-800 nm. The ratio of the fluorescence intensities at 620 nm and 554 nm was obtained for ethanol concentrations varying from 0-100% and the calibration curve of the ratiometric fluorescence intensities over the entire concentration range of ethanol was plotted. A ratiometric intensity change of over 33% has been obtained for pure ethanol compared to that obtained for pure water. The sensor response was rapid (≤10 minutes). The sterilizable ethanol sensor exhibits good potential for on-line monitoring of the ethanol generated in an LB fermentation chamber. |
topic |
Alcohol sensing Optical measurements Fluorescence Ratiometric sensor |
url |
http://www.clbme.bas.bg/bioautomation/2008/vol_9.1/files/9_2.3.pdf |
work_keys_str_mv |
AT sherifibrahim ratiometricalcoholsensorbasedonapolymericnileblue AT nirmalachandrasekharan ratiometricalcoholsensorbasedonapolymericnileblue AT yordankostov ratiometricalcoholsensorbasedonapolymericnileblue AT govindrao ratiometricalcoholsensorbasedonapolymericnileblue |
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1724621336637079552 |