Results of the microbiota assessment in experimental ulcerative colitis

• Main Authors: A. D. Kim, S. A. Lepekhova, E. Y. Chashkova, E. V. Koval, Yu. I. Pivovarov, T. V. Fadeeva, O. A. Goldberg
• Format: Article
• Language: English
• Published: Siberian State Medical University (Tomsk) 2021-04-01
• Series: Bûlleten' Sibirskoj Mediciny
• Subjects: ulcerative colitis; bacteriology; microflora; experiment
• Online Access: https://bulletin.tomsk.ru/jour/article/view/4278

Background. The increased incidence of inflammatory bowel disease (IBD) in the world and the lack of consensus on the causes and development mechanisms of IBD are the key elements that determine the relevance of the study. According to some authors, in the pathogenesis of the development and occurrence of ulcerative colitis, one of the leading causes is a change in the composition of the colon microflora and the impact of the products of their metabolism on the enteric system and intestinal motility.The aim was to study the qualitative and quantitative changes in the colon microbiota in rats when modeling ulcerative lesions by the original method.Materials and methods. The experimental study was carried out using male Wistar rats (n = 24). Ulcerative colitis was modeled in an original way. The quantitative and qualitative composition of the parietal microflora of the distal colon was determined.Results. When modeling ulcerative lesions of the colon using the original method, changes in the qualitative and quantitative composition of the parietal microflora of the colon were revealed. On the 3rd day there was a decrease in Lactobacillus ssp. and Escherichia coli, as well as the growth of  fungal microflora and the appearance of representatives of opportunistic microflora. The changes were progressive in nature, and by the 7th day of the study revealed a marked reduction of the total parietal concentration of the normal flora bacteria and an increased percentage and absolute number of representatives of conditionally pathogenic microflora. By the 10th day of the experiment, with a small increase in the total number of parietal bacteria, the  predominant microorganisms were Bacteroides ssp. (26.8%) and Peptococcus ssp. (27.6%).