Pyrrolidine-based 3-deoxysphingosylphosphorylcholine analogs as possible candidates against neglected tropical diseases (NTDs): identification of hit compounds towards development of potential treatment of Leishmania donovani
A rational-based process was adopted for repurposing pyrrolidine-based 3-deoxysphingosylphosphorylcholine analogs bearing variable acyl chains, different stereochemical configuration and/or positional relationships. Structural features were highly influential on activity. Amongst, enantiomer 1e havi...
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doaj-33781956a9284f9394ef0a3eb388e31d2021-09-06T14:06:25ZengTaylor & Francis GroupJournal of Enzyme Inhibition and Medicinal Chemistry1475-63661475-63742021-01-013611922193010.1080/14756366.2021.19693851969385Pyrrolidine-based 3-deoxysphingosylphosphorylcholine analogs as possible candidates against neglected tropical diseases (NTDs): identification of hit compounds towards development of potential treatment of Leishmania donovaniAhmed H. E. Hassan0Trong-Nhat Phan1Seolmin Yoon2Cheol Jung Lee3Hye Rim Jeon4Seung-Hwan Kim5Joo Hwan No6Yong Sup Lee7Department of Medicinal Chemistry, Faculty of Pharmacy, Mansoura UniversityLeishmania Research Laboratory, Institut Pasteur KoreaDepartment of Life and Nanopharmaceutical Sciences, Kyung Hee UniversityDepartment of Life and Nanopharmaceutical Sciences, Kyung Hee UniversityDepartment of Life and Nanopharmaceutical Sciences, Kyung Hee UniversityDepartment of Life and Nanopharmaceutical Sciences, Kyung Hee UniversityLeishmania Research Laboratory, Institut Pasteur KoreaMedicinal Chemistry Laboratory, College of Pharmacy, Kyung Hee UniversityA rational-based process was adopted for repurposing pyrrolidine-based 3-deoxysphingosylphosphorylcholine analogs bearing variable acyl chains, different stereochemical configuration and/or positional relationships. Structural features were highly influential on activity. Amongst, enantiomer 1e having 1,2-vicinal relationship for the -CH2O- and the N-acyl moieties, a saturated palmitoyl chain and an opposite stereochemical configuration to natural sphingolipids was the most potent hit compound against promastigotes showing IC50 value of 28.32 µM. The corresponding enantiomer 1a was 2-fold less potent showing a eudismic ratio of 0.54 in promastigotes. Compounds 1a and 1e inhibited the growth of amastigotes more potently relative to promastigotes. Amongst, enantiomer 1a as the more selective and safer. In silico docking study using a homology model of Leishmania donovani inositol phosphoceramide synthase (IPCS) provided plausible reasoning for the molecular factors underlying the found activity. Collectively, this study suggests compounds 1a and 1e as potential hit compounds for further development of new antileishmanial agents.http://dx.doi.org/10.1080/14756366.2021.1969385antileishmanial agentspromastigotesamastigotesrepurposingmolecular dockinginositol phosphoceramide synthaseipcssphingomyelin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ahmed H. E. Hassan Trong-Nhat Phan Seolmin Yoon Cheol Jung Lee Hye Rim Jeon Seung-Hwan Kim Joo Hwan No Yong Sup Lee |
spellingShingle |
Ahmed H. E. Hassan Trong-Nhat Phan Seolmin Yoon Cheol Jung Lee Hye Rim Jeon Seung-Hwan Kim Joo Hwan No Yong Sup Lee Pyrrolidine-based 3-deoxysphingosylphosphorylcholine analogs as possible candidates against neglected tropical diseases (NTDs): identification of hit compounds towards development of potential treatment of Leishmania donovani Journal of Enzyme Inhibition and Medicinal Chemistry antileishmanial agents promastigotes amastigotes repurposing molecular docking inositol phosphoceramide synthase ipcs sphingomyelin |
author_facet |
Ahmed H. E. Hassan Trong-Nhat Phan Seolmin Yoon Cheol Jung Lee Hye Rim Jeon Seung-Hwan Kim Joo Hwan No Yong Sup Lee |
author_sort |
Ahmed H. E. Hassan |
title |
Pyrrolidine-based 3-deoxysphingosylphosphorylcholine analogs as possible candidates against neglected tropical diseases (NTDs): identification of hit compounds towards development of potential treatment of Leishmania donovani |
title_short |
Pyrrolidine-based 3-deoxysphingosylphosphorylcholine analogs as possible candidates against neglected tropical diseases (NTDs): identification of hit compounds towards development of potential treatment of Leishmania donovani |
title_full |
Pyrrolidine-based 3-deoxysphingosylphosphorylcholine analogs as possible candidates against neglected tropical diseases (NTDs): identification of hit compounds towards development of potential treatment of Leishmania donovani |
title_fullStr |
Pyrrolidine-based 3-deoxysphingosylphosphorylcholine analogs as possible candidates against neglected tropical diseases (NTDs): identification of hit compounds towards development of potential treatment of Leishmania donovani |
title_full_unstemmed |
Pyrrolidine-based 3-deoxysphingosylphosphorylcholine analogs as possible candidates against neglected tropical diseases (NTDs): identification of hit compounds towards development of potential treatment of Leishmania donovani |
title_sort |
pyrrolidine-based 3-deoxysphingosylphosphorylcholine analogs as possible candidates against neglected tropical diseases (ntds): identification of hit compounds towards development of potential treatment of leishmania donovani |
publisher |
Taylor & Francis Group |
series |
Journal of Enzyme Inhibition and Medicinal Chemistry |
issn |
1475-6366 1475-6374 |
publishDate |
2021-01-01 |
description |
A rational-based process was adopted for repurposing pyrrolidine-based 3-deoxysphingosylphosphorylcholine analogs bearing variable acyl chains, different stereochemical configuration and/or positional relationships. Structural features were highly influential on activity. Amongst, enantiomer 1e having 1,2-vicinal relationship for the -CH2O- and the N-acyl moieties, a saturated palmitoyl chain and an opposite stereochemical configuration to natural sphingolipids was the most potent hit compound against promastigotes showing IC50 value of 28.32 µM. The corresponding enantiomer 1a was 2-fold less potent showing a eudismic ratio of 0.54 in promastigotes. Compounds 1a and 1e inhibited the growth of amastigotes more potently relative to promastigotes. Amongst, enantiomer 1a as the more selective and safer. In silico docking study using a homology model of Leishmania donovani inositol phosphoceramide synthase (IPCS) provided plausible reasoning for the molecular factors underlying the found activity. Collectively, this study suggests compounds 1a and 1e as potential hit compounds for further development of new antileishmanial agents. |
topic |
antileishmanial agents promastigotes amastigotes repurposing molecular docking inositol phosphoceramide synthase ipcs sphingomyelin |
url |
http://dx.doi.org/10.1080/14756366.2021.1969385 |
work_keys_str_mv |
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