Acupuncture Attenuates Anxiety-Like Behavior by Normalizing Amygdaloid Catecholamines during Ethanol Withdrawal in Rats
Previously, we demonstrated acupuncture at acupoint HT7 (Shen-Men) attenuated ethanol withdrawal syndrome by normalizing the dopamine release in nucleus accumbens shell. In the present study, we investigated the effect of acupuncture on anxiety-like behavior in rats and its relevant mechanism by stu...
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2011-01-01
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Online Access: | http://dx.doi.org/10.1093/ecam/neq045 |
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doaj-337db59b5dbc4c0aa9249a95e71d72d32020-11-24T23:11:59ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882011-01-01201110.1093/ecam/neq045429843Acupuncture Attenuates Anxiety-Like Behavior by Normalizing Amygdaloid Catecholamines during Ethanol Withdrawal in RatsZheng Lin Zhao0Guang Wen Zhao1Hou Zhong Li2Xu Dong Yang3Yi Yan Wu4Feng Lin5Li Xin Guan6Feng Guo Zhai7Jia Qi Liu8Chae Ha Yang9Sang Chan Kim10Kee Won Kim11Rong Jie Zhao12Department of Pharmacology, Mudanjiang Medical University, Mudanjiang 157011, ChinaDepartment of Surgery, Medical College of Yanbian University, Yanji 133000, ChinaDepartment of Pharmacology, Mudanjiang Medical University, Mudanjiang 157011, ChinaDepartment of Pharmacology, Mudanjiang Medical University, Mudanjiang 157011, ChinaDepartment of Pharmacology, Mudanjiang Medical University, Mudanjiang 157011, ChinaDepartment of Pharmacology, Mudanjiang Medical University, Mudanjiang 157011, ChinaDepartment of Pharmacology, Mudanjiang Medical University, Mudanjiang 157011, ChinaDepartment of Pharmacology, Mudanjiang Medical University, Mudanjiang 157011, ChinaDepartment of Pharmacology, Mudanjiang Medical University, Mudanjiang 157011, ChinaThe Research Center for Biomedical Resource of Oriental Medicine, Daegu Haany University, Daegu 706-828, Republic of KoreaThe Research Center for Biomedical Resource of Oriental Medicine, Daegu Haany University, Daegu 706-828, Republic of KoreaDepartment of Pharmacology, Medical School of Chonbuk National University, Chonju, Chonbuk 561-180, Republic of KoreaDepartment of Pharmacology, Mudanjiang Medical University, Mudanjiang 157011, ChinaPreviously, we demonstrated acupuncture at acupoint HT7 (Shen-Men) attenuated ethanol withdrawal syndrome by normalizing the dopamine release in nucleus accumbens shell. In the present study, we investigated the effect of acupuncture on anxiety-like behavior in rats and its relevant mechanism by studying neuro-endocrine parameters during ethanol withdrawal. Rats were treated with 3 g kg−1day−1 of ethanol (20%, w/v) or saline by intraperitoneal injections for 28 days. The rats undergoing ethanol withdrawal exhibited anxiety-like behavior 72 h after the last dose of ethanol characterized by the decrease of time spent in the open arms of the elevated plus maze compared with the saline-treated rats (P < .05). Radioimmunoassay exhibited there were notably increased concentrations of plasma corticosterone in ethanol-withdrawn rats compared with saline-treated rats (P < .05). Additionally, high performance liquid chromatography analysis also showed the levels of norepinephrine and 3-methoxy-4-hydroxy-phenylglycol were markedly increased while the levels of dopamine and 3,4-dihydroxyphenylacetic acid were significantly decreased in the central nucleus of the amygdala of ethanol-withdrawn rats compared with saline-treated rats (P < .01). Acupuncture groups were treated with acupuncture at acupoint HT7 or PC6 (Nei-Guan). Acupuncture at HT7 but not PC6 greatly attenuated the anxiety-like behavior during ethanol withdrawal as evidenced by significant increases in the percentage of time spent in open arms (P < .05). In the meantime, acupuncture at HT7 also markedly inhibited the alterations of neuro-endocrine parameters induced by ethanol withdrawal (P < .05). These results suggest that acupuncture may attenuate anxiety-like behavior during ethanol withdrawal through regulation of neuro-endocrine system.http://dx.doi.org/10.1093/ecam/neq045 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zheng Lin Zhao Guang Wen Zhao Hou Zhong Li Xu Dong Yang Yi Yan Wu Feng Lin Li Xin Guan Feng Guo Zhai Jia Qi Liu Chae Ha Yang Sang Chan Kim Kee Won Kim Rong Jie Zhao |
spellingShingle |
Zheng Lin Zhao Guang Wen Zhao Hou Zhong Li Xu Dong Yang Yi Yan Wu Feng Lin Li Xin Guan Feng Guo Zhai Jia Qi Liu Chae Ha Yang Sang Chan Kim Kee Won Kim Rong Jie Zhao Acupuncture Attenuates Anxiety-Like Behavior by Normalizing Amygdaloid Catecholamines during Ethanol Withdrawal in Rats Evidence-Based Complementary and Alternative Medicine |
author_facet |
Zheng Lin Zhao Guang Wen Zhao Hou Zhong Li Xu Dong Yang Yi Yan Wu Feng Lin Li Xin Guan Feng Guo Zhai Jia Qi Liu Chae Ha Yang Sang Chan Kim Kee Won Kim Rong Jie Zhao |
author_sort |
Zheng Lin Zhao |
title |
Acupuncture Attenuates Anxiety-Like Behavior by Normalizing Amygdaloid Catecholamines during Ethanol Withdrawal in Rats |
title_short |
Acupuncture Attenuates Anxiety-Like Behavior by Normalizing Amygdaloid Catecholamines during Ethanol Withdrawal in Rats |
title_full |
Acupuncture Attenuates Anxiety-Like Behavior by Normalizing Amygdaloid Catecholamines during Ethanol Withdrawal in Rats |
title_fullStr |
Acupuncture Attenuates Anxiety-Like Behavior by Normalizing Amygdaloid Catecholamines during Ethanol Withdrawal in Rats |
title_full_unstemmed |
Acupuncture Attenuates Anxiety-Like Behavior by Normalizing Amygdaloid Catecholamines during Ethanol Withdrawal in Rats |
title_sort |
acupuncture attenuates anxiety-like behavior by normalizing amygdaloid catecholamines during ethanol withdrawal in rats |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2011-01-01 |
description |
Previously, we demonstrated acupuncture at acupoint HT7 (Shen-Men) attenuated ethanol withdrawal syndrome by normalizing the dopamine release in nucleus accumbens shell. In the present study, we investigated the effect of acupuncture on anxiety-like behavior in rats and its relevant mechanism by studying neuro-endocrine parameters during ethanol withdrawal. Rats were treated with 3 g kg−1day−1 of ethanol (20%, w/v) or saline by intraperitoneal injections for 28 days. The rats undergoing ethanol withdrawal exhibited anxiety-like behavior 72 h after the last dose of ethanol characterized by the decrease of time spent in the open arms of the elevated plus maze compared with the saline-treated rats (P < .05). Radioimmunoassay exhibited there were notably increased concentrations of plasma corticosterone in ethanol-withdrawn rats compared with saline-treated rats (P < .05). Additionally, high performance liquid chromatography analysis also showed the levels of norepinephrine and 3-methoxy-4-hydroxy-phenylglycol were markedly increased while the levels of dopamine and 3,4-dihydroxyphenylacetic acid were significantly decreased in the central nucleus of the amygdala of ethanol-withdrawn rats compared with saline-treated rats (P < .01). Acupuncture groups were treated with acupuncture at acupoint HT7 or PC6 (Nei-Guan). Acupuncture at HT7 but not PC6 greatly attenuated the anxiety-like behavior during ethanol withdrawal as evidenced by significant increases in the percentage of time spent in open arms (P < .05). In the meantime, acupuncture at HT7 also markedly inhibited the alterations of neuro-endocrine parameters induced by ethanol withdrawal (P < .05). These results suggest that acupuncture may attenuate anxiety-like behavior during ethanol withdrawal through regulation of neuro-endocrine system. |
url |
http://dx.doi.org/10.1093/ecam/neq045 |
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