Combination Effect of Caffeine and Caffeic Acid Treatment on the Oxidant Status of Ectopic Endometrial Cells Separated from Patients with Endometriosis
Background: Endometriosis is a common gynecological disease in which oxidative stress is a potential factor. Caffeine and caffeic acid are present in various foods and beverages with anti-oxidant, anti-inflammatory, and anti-carcinogenic properties. In this study, we aimed to investigate the amelior...
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Shiraz University of Medical Sciences
2019-07-01
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doaj-33981d435620439a98ad8dfb530226e12020-11-25T02:20:50ZengShiraz University of Medical SciencesIranian Journal of Medical Sciences0253-07161735-36882019-07-0144431532410.30476/ijms.2019.4497044970Combination Effect of Caffeine and Caffeic Acid Treatment on the Oxidant Status of Ectopic Endometrial Cells Separated from Patients with EndometriosisNavid Jamali0Zohreh Mostafavi-Pour1Fatemeh Zal2Maryam Kasraeian3Tahereh Poordast4Fatemeh Ramezani5Razieh Zare6Biochemistry Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; and Student Research Committee, Shiraz University of Medical Sciences, Shiraz, IranBiochemistry Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; and Maternal-Fetal Medicine Research Center, Shiraz University of Medical Sciences, Shiraz, IranBiochemistry Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; and Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, IranMaternal-Fetal Medicine Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; and Department of OB/GYN, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranInfertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; and Department of OB/GYN, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranBiochemistry Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; and Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, IranBiochemistry Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranBackground: Endometriosis is a common gynecological disease in which oxidative stress is a potential factor. Caffeine and caffeic acid are present in various foods and beverages with anti-oxidant, anti-inflammatory, and anti-carcinogenic properties. In this study, we aimed to investigate the ameliorative effects of caffeine, caffeic acid, and caffeine+caffeic acid treatments on oxidative stress in ectopic endometrial cells taken from patients and eutopic ones from women without endometriosis.Methods: In this experimental study, eutopic and ectopic endometrial cells were obtained from biopsies of women free of disease (n=10) and patients with endometriosis (n=10) who referred to Shiraz reference hospitals (2017-2018). Both eutopic and ectopic endometrial cells were divided into four groups: Treated with caffeine, with caffeic acid, with caffeine+caffeic acid, and the control. Also, antioxidant enzyme activities and the levels of glutathione (GSH) and malondialdehyde (MDA) were determined in each group. The data were analyzed using independent sample t test and one-way ANOVA followed by Tukey post-hoc test.Results: Caffeic acid, but not caffeine treatment demonstrated a decrease in MDA level (P<0.001) as well as an increase in GSH level (P<0.001) and antioxidant enzyme activities in ectopic endometrial cells. Also, the treatment of the cells with caffeine+caffeic acid caused similar effects as those ectopic cells treated with caffeic acid. Conclusion: According to the findings of the present study, caffeic acid reduced oxidative stress which may alleviate the complications associated with endometriosis. However, more investigations are needed for evaluating the efficiency and safety of caffeic acid.http://ijms.sums.ac.ir/article_44970_5f7345db6a766bd197e82f1b10faa0dd.pdfEndometriosisOxidative stressCaffeineCaffeic acid |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Navid Jamali Zohreh Mostafavi-Pour Fatemeh Zal Maryam Kasraeian Tahereh Poordast Fatemeh Ramezani Razieh Zare |
spellingShingle |
Navid Jamali Zohreh Mostafavi-Pour Fatemeh Zal Maryam Kasraeian Tahereh Poordast Fatemeh Ramezani Razieh Zare Combination Effect of Caffeine and Caffeic Acid Treatment on the Oxidant Status of Ectopic Endometrial Cells Separated from Patients with Endometriosis Iranian Journal of Medical Sciences Endometriosis Oxidative stress Caffeine Caffeic acid |
author_facet |
Navid Jamali Zohreh Mostafavi-Pour Fatemeh Zal Maryam Kasraeian Tahereh Poordast Fatemeh Ramezani Razieh Zare |
author_sort |
Navid Jamali |
title |
Combination Effect of Caffeine and Caffeic Acid Treatment on the Oxidant Status of Ectopic Endometrial Cells Separated from Patients with Endometriosis |
title_short |
Combination Effect of Caffeine and Caffeic Acid Treatment on the Oxidant Status of Ectopic Endometrial Cells Separated from Patients with Endometriosis |
title_full |
Combination Effect of Caffeine and Caffeic Acid Treatment on the Oxidant Status of Ectopic Endometrial Cells Separated from Patients with Endometriosis |
title_fullStr |
Combination Effect of Caffeine and Caffeic Acid Treatment on the Oxidant Status of Ectopic Endometrial Cells Separated from Patients with Endometriosis |
title_full_unstemmed |
Combination Effect of Caffeine and Caffeic Acid Treatment on the Oxidant Status of Ectopic Endometrial Cells Separated from Patients with Endometriosis |
title_sort |
combination effect of caffeine and caffeic acid treatment on the oxidant status of ectopic endometrial cells separated from patients with endometriosis |
publisher |
Shiraz University of Medical Sciences |
series |
Iranian Journal of Medical Sciences |
issn |
0253-0716 1735-3688 |
publishDate |
2019-07-01 |
description |
Background: Endometriosis is a common gynecological disease in which oxidative stress is a potential factor. Caffeine and caffeic acid are present in various foods and beverages with anti-oxidant, anti-inflammatory, and anti-carcinogenic properties. In this study, we aimed to investigate the ameliorative effects of caffeine, caffeic acid, and caffeine+caffeic acid treatments on oxidative stress in ectopic endometrial cells taken from patients and eutopic ones from women without endometriosis.Methods: In this experimental study, eutopic and ectopic endometrial cells were obtained from biopsies of women free of disease (n=10) and patients with endometriosis (n=10) who referred to Shiraz reference hospitals (2017-2018). Both eutopic and ectopic endometrial cells were divided into four groups: Treated with caffeine, with caffeic acid, with caffeine+caffeic acid, and the control. Also, antioxidant enzyme activities and the levels of glutathione (GSH) and malondialdehyde (MDA) were determined in each group. The data were analyzed using independent sample t test and one-way ANOVA followed by Tukey post-hoc test.Results: Caffeic acid, but not caffeine treatment demonstrated a decrease in MDA level (P<0.001) as well as an increase in GSH level (P<0.001) and antioxidant enzyme activities in ectopic endometrial cells. Also, the treatment of the cells with caffeine+caffeic acid caused similar effects as those ectopic cells treated with caffeic acid. Conclusion: According to the findings of the present study, caffeic acid reduced oxidative stress which may alleviate the complications associated with endometriosis. However, more investigations are needed for evaluating the efficiency and safety of caffeic acid. |
topic |
Endometriosis Oxidative stress Caffeine Caffeic acid |
url |
http://ijms.sums.ac.ir/article_44970_5f7345db6a766bd197e82f1b10faa0dd.pdf |
work_keys_str_mv |
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