Cytotoxicity of Buah Merah (Pandanus conoideus Lamk.) Extract on Breast Cancer Cell Line (T47D)

• Main Authors: Tri R. Nuringtyas, Yoga Pratama, G. Galih, Subagus Wahyuono, Sukarti Moeljopawiro
• Format: Article
• Language: English
• Published: Universitas Gadjah Mada, Yogyakarta 2014-06-01
• Series: Indonesian Journal of Biotechnology
• Subjects: Buah Merah; cytotoxicity; T47D; vacuum liquid chromatography; thin-layer chromatography
• Online Access: https://jurnal.ugm.ac.id/ijbiotech/article/view/8636

Buah Merah (Pandanus conoideus Lamk.) has been extensively used to treat various diseases including cancer. There are many varieties of buah merah and there was no scientifi c study comparing cytotoxicity of different varieties. The objective of this study was to investigate the cytotoxicity of three varieties of buah merah known as Barugum, Maler and Yanggiru on breast cancer cell line (T47D). All samples were collected from Papua, Indonesia. Each sample was extracted consecutively using three solvents chloroform, methanol and water resulted to nine crude extracts. The cytotoxic activities were determined using MTT assay. The crude extract showed the lowest IC50 was selected for further bioassay-guided fractionation. Fractionation was done using vacuum liquid chromatography coupled with preparative TLC to fi nd the active compounds. Several detection reagents were applied to TLC for identifi cation of the class of the potent compounds. The result showed that the potent extracts was obtained from Barugum methanol extract followed by Maler chloroform extract with IC50 value of 132.83 μg/ml and 139.72 μg/ml, respectively. All Yanggiru extracts did not show activity. The bioassay-guided fractionation of Barugum and Maler extracts showed that the most potent fraction eluted by a mixture of hexane:ethyl acetate (75:25), was in Maler variety with IC50 value of 25,7 μg/ml, four times higher than the most potent fraction of Barugum with IC50 value of 104,61 μg/ml. TLC analysis of the most potent fraction showed that the active compounds was class of terpene. Result of this study supported the utilization of buah merah Maler variety for breast cancer treatment.